公开(公告)号：US20230055008A1

公开(公告)日：2023-02-23

申请号：US17757986

申请日：2020-08-06

Applicant: XIAMEN UNIVERSITY

Inventor： Qiuying HUANG ,  Qingge LI

IPC: C12Q1/686 ,  C12Q1/6876

Abstract: Provided is a method for multiplex and asymmetric amplification of one or more target nucleic acids in a sample. The method can simultaneously amplify multiple target nucleic acids existing in a sample, and can simultaneously produce a large number of single-chain products.

公开(公告)号：US20240076739A1

公开(公告)日：2024-03-07

申请号：US18267840

申请日：2020-12-29

Applicant: XIAMEN UNIVERSITY

Inventor： Qingge LI ,  Qiuying HUANG ,  Xinwen CHEN

IPC: C12Q1/6881 ,  C12Q1/6858

CPC classification number: C12Q1/6881 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: A method for detecting SNP sites of a donor-derived sample and a recipient-derived sample, a method for detecting the presence or proportion of a donor in a receptor sample, and a kit for implementing the methods.

公开(公告)号：US20140162898A1

公开(公告)日：2014-06-12

申请号：US14182067

申请日：2014-02-17

Applicant: XIAMEN UNIVERSITY

Inventor： Qingge LI ,  Qiuying HUANG

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/6827 ,  C12Q2527/107 ,  C12Q2531/107 ,  C12Q2535/131

Abstract: The present invention relates to a method and a kit for detecting nucleic acid sequence variation using melting curve analysis, especially relates to a method and a kit for detecting nucleic acid sequence variation by melting curve analysis using self-quenched probe. Said method provides the characteristics of the self-quenched probe employed, as well as the corresponding nucleic acid amplification conditions, so that the probe can bind to the amplified target sequence, and variations of the target sequence can be detected by melting curve analysis. The present invention also encompasses a kit assembled according to the method described.

Abstract translation: 本发明涉及使用熔解曲线分析检测核酸序列变异的方法和试剂盒，特别涉及通过使用自淬灭探针的熔解曲线分析来检测核酸序列变异的方法和试剂盒。 所述方法提供了使用的自淬灭探针的特征，以及相应的核酸扩增条件，使得探针可以结合扩增的靶序列，并且可以通过熔解曲线分析来检测靶序列的变化。 本发明还包括根据所述方法组装的试剂盒。

公开(公告)号：US20220364146A1

公开(公告)日：2022-11-17

申请号：US17757982

申请日：2020-07-22

Applicant: XIAMEN UNIVERSITY

Inventor： Qiuying HUANG ,  Qingge LI

IPC: C12Q1/686

Abstract: Provided is multiplex and asymmetric amplification of nucleic acid molecules. In particular, provided is a method for simultaneous and asymmetric amplification of one or more target nucleic acids in a sample. The method can simultaneously and asymmetrically amplify multiple target nucleic acids existing in a sample, and can simultaneously produce large number of single stranded products.

公开(公告)号：US20220017949A1

公开(公告)日：2022-01-20

申请号：US17392027

申请日：2021-08-02

Applicant: XIAMEN UNIVERSITY

Inventor： Qingge LI ,  Dongmei CHEN ,  Qiuying HUANG ,  Ye XU ,  Yiqun LIAO

IPC: C12Q1/6818 ,  C12N15/11

Abstract: The present application relates to multiplex detection of nucleic acid molecules. In particular, the present application provides a method for detecting target nucleic acid sequences, said method can simultaneously detect the presence of multiple target nucleic acid sequences in a sample. In addition, the present application further provides a probe set, and a kit comprising one or more said probe sets, said probe set and said kit can be used to carry out the method of the invention.