公开(公告)号：US11111522B2

公开(公告)日：2021-09-07

申请号：US16327256

申请日：2018-04-27

Applicant: XIAMEN UNIVERSITY

Inventor： Qingge Li ,  Dongmei Chen ,  Qiuying Huang ,  Ye Xu ,  Yiqun Liao

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12N15/11

Abstract: The present application relates to multiplex detection of nucleic acid molecules. In particular, the present application provides a method for detecting target nucleic acid sequences, said method can simultaneously detect the presence of multiple target nucleic acid sequences in a sample. In addition, the present application further provides a probe set, and a kit comprising one or more said probe sets, said probe set and said kit can be used to carry out the method of the invention.

公开(公告)号：US09334533B2

公开(公告)日：2016-05-10

申请号：US14182067

申请日：2014-02-17

Applicant: XIAMEN UNIVERSITY

Inventor： Qingge Li ,  Qiuying Huang

IPC: C12Q1/68 ,  C07H21/00

CPC classification number: C12Q1/6858 ,  C12Q1/6827 ,  C12Q2527/107 ,  C12Q2531/107 ,  C12Q2535/131

Abstract: The present invention relates to a method and a kit for detecting nucleic acid sequence variation using melting curve analysis, especially relates to a method and a kit for detecting nucleic acid sequence variation by melting curve analysis using self-quenched probe. Said method provides the characteristics of the self-quenched probe employed, as well as the corresponding nucleic acid amplification conditions, so that the probe can bind to the amplified target sequence, and variations of the target sequence can be detected by melting curve analysis. The present invention also encompasses a kit assembled according to the method described.

Abstract translation: 本发明涉及使用熔解曲线分析检测核酸序列变异的方法和试剂盒，特别涉及通过使用自淬灭探针的熔解曲线分析来检测核酸序列变异的方法和试剂盒。 所述方法提供了使用的自淬灭探针的特征，以及相应的核酸扩增条件，使得探针可以结合扩增的靶序列，并且可以通过熔解曲线分析来检测靶序列的变化。 本发明还包括根据所述方法组装的试剂盒。