公开(公告)号：US20190055506A1

公开(公告)日：2019-02-21

申请号：US15573381

申请日：2016-05-11

Applicant: XIAMEN UNIVERSITY ,  XIAMEN INNODX BIOTECH CO., LTD

Inventor： Shengxiang GE ,  Shiyin ZHANG ,  Feihai XU ,  Jin WANG ,  Jinjie LI ,  Jun ZHANG ,  Ningshao XIA

IPC: C12M1/38 ,  C12M1/24 ,  C12Q1/6844 ,  B01L3/00

Abstract: Disclosed are a reaction tube for nucleic acid amplification capable of controlling a liquid circulation path, a reaction apparatus for nucleic acid amplification comprising the reaction tube, and a method for amplifying nucleic acid comprising a step of using the reaction tube. Also disclosed are a kit comprising the reaction tube, and use of the reaction tube in preparation of a kit.

公开(公告)号：US20190316176A1

公开(公告)日：2019-10-17

申请号：US16314356

申请日：2017-06-29

Applicant: Xiamen University

Inventor： Shiyin ZHANG ,  Shengxiang GE ,  Jin WANG ,  Jun ZHANG ,  Ningshao XIA

IPC: C12Q1/686 ,  C12N15/10

Abstract: A ribonucleic acid (RNA) reverse transcription amplification method, comprising the steps of reverse transcription of RNA into cDNA and immediate amplification of cDNA, characterized in that the process of reverse transcription of RNA into cDNA is completed during the process that a cDNA amplification reaction system is heated to reach the reaction condition for cDNA amplification. The RNA reverse transcription amplification method can combine the reaction condition for reverse transcription of RNA into cDNA with the reaction condition for cDNA amplification, thereby significantly shorten the time required for RNA reverse transcription amplification. And in the whole process of RNA reverse transcription amplification, there is no need to change the instrument temperature, and thus, it is possible to achieve detection at any time.