公开(公告)号：US20090117108A1

公开(公告)日：2009-05-07

申请号：US10594908

申请日：2005-03-29

申请人： Xiangbin Wang ,  Hualiang Huang ,  Baofeng Zhao ,  Qi Zhao ,  Jinhua Piao ,  Qing Lin

发明人： Xiangbin Wang ,  Hualiang Huang ,  Baofeng Zhao ,  Qi Zhao ,  Jinhua Piao ,  Qing Lin

IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21 ,  C12P21/04

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/3007 ,  C07K2317/34 ,  C07K2317/626 ,  C07K2317/73

摘要： The invention is related to a recombinant single-chain tri-specific antibody made from anti-Tumor Associated Antigen (TAA) antibody, FC interlinker, anti-CD3 antibody, HSA interlinker and anti-CD28 antibody in turn. Particularly, the invention relates to an anti-CEA, anti-CD3, anti-CD28 recombinant single-chain tri-specific antibody, CEA-scTsAb, which was constructed with three tandem antibody fragments (anti-CEA scFv, anti-CD3 scFv and anti-CD28 single-domain antibody) linked by two interlinkers (FC interlinker, HSA interlinker), and could be appended by C myc tag or histidine tag ((His)6-tag) at the C terminal. It also concerns a method for construction, expression and purification of the antibody. It also offers the encoded DNA sequence of the antibody, expression vectors and host cells for the vectors.

摘要翻译： 本发明涉及由抗肿瘤相关抗原（TAA）抗体，FC连接子，抗CD3抗体，HSA互连体和抗CD28抗体依次制备的重组单链三特异性抗体。 特别地，本发明涉及用三个串联抗体片段（抗CEA scFv，抗CD3 scFv和构建体）构建的抗CEA，抗CD3，抗CD28重组单链三特异性抗体CEA-scTsAb 抗CD28单结构域抗体）通过两个连接子（FC连接子，HSA连接子）连接，并可以通过C末端标签或组氨酸标签（（His）6-标签）附加。 它还涉及抗体的构建，表达和纯化的方法。 它还提供抗体的编码DNA序列，载体的表达载体和宿主细胞。