公开(公告)号：US20200207808A1

公开(公告)日：2020-07-02

申请号：US16729025

申请日：2019-12-27

Applicant: Xin WEN

Inventor： Xin WEN

IPC: C07K1/36 ,  C07K1/02 ,  C07K14/195

Abstract: A method of purifying an antifreeze protein (AFP) and methods of producing AFPs are disclosed. The method of purifying an AFP includes heating a crude AFP in an aqueous medium to a temperature and for a length of time sufficient to precipitate at least some thermally unstable proteins in the crude AFP and form a precipitate and a supernatant; and removing the precipitate from the supernatant. One method of producing an AFP includes collecting a crude source of the AFP; and purifying the AFP by the purification method. Another method of producing an AFP includes growing or culturing a host configured to express a recombinant AFP in a growth medium, and collecting a crude AFP from the host and/or the growth medium. The growth medium comprises water, a protein hydrolysate or other source of amino acids, a yeast extract, a biologically metabolizable C3-C6 polyol, and one or more phosphate salts.

公开(公告)号：US20180133329A1

公开(公告)日：2018-05-17

申请号：US15807405

申请日：2017-11-08

Applicant: Xin WEN ,  Sen WANG

Inventor： Xin WEN ,  Sen WANG

IPC: A61K47/42 ,  A61K47/26 ,  A61K9/19

CPC classification number: A61K47/42 ,  A61K9/19 ,  A61K47/26

Abstract: The disclosure pertains to compositions to stabilize nutrient, food, pharmaceutical, and biological formulations by preventing the crystallization or precipitation of carbohydrates used in the formulations. The compositions contain (i) one or more carbohydrates, sugars, sugar alcohols, analogs thereof, and/or derivatives thereof and (ii) one or more antifreeze proteins (AFPs), mimetics thereof, and/or analogs thereof and methods of making and using the same. The AFP may be selected from antifreeze polypeptides and antifreeze peptides, analogs and mimetics of antifreeze proteins, active fragments of such antifreeze proteins, polypeptide and peptide mimetics, antifreeze peptoids and polymers, and combinations thereof. The carbohydrate, sugar or sugar alcohol has the formula CmHnOp, where m is an integer of 5 or 6, n is an integer of 2m or 2m+2, and p is equal to m, or the formula CaHbOc, where a is an integer of 10-12 or 15-18, b is 2a−2 when a is an integer of 10-12 or 2a−4 when a is an integer of 15-18, and c is a−1 when a is an integer of 10-12 or a−2 when a is an integer of 15-18. The AFP, AFP analog, or AFP mimetic inhibits or controls crystallization of the carbohydrate (e.g., during the storage of the solution of the carbohydrate or the analog or derivative thereof, during the freeze-drying of a formulation using the carbohydrate or the analog thereof) and stabilize the solution or the formulation using the carbohydrate or the carbohydrate analog or derivative.

公开(公告)号：US20180147287A1

公开(公告)日：2018-05-31

申请号：US15821671

申请日：2017-11-22

Applicant: Xin WEN

Inventor： Xin WEN

IPC: A61K47/42 ,  C12N9/96 ,  A61K38/28 ,  A01N1/02 ,  C12N1/04

CPC classification number: A61K47/42 ,  A01N1/0221 ,  A61K38/28 ,  C12N1/04 ,  C12N9/96

Abstract: Embodiments of the disclosure pertain to compositions containing (i) one or more non-antifreeze proteins or cell components or cells and (ii) one or more antifreeze proteins (AFPs), mimetics thereof, and/or analogs thereof, and a method of stabilizing a biologic (e.g., a protein, microbe, cell component or cell) against temperature stress and aggregation. The AFP may be selected from known antifreeze polypeptides and antifreeze peptides, analogs and mimetics of antifreeze proteins, active fragments of such antifreeze proteins, polypeptide and peptide mimetics, antifreeze peptoids and polymers, and combinations thereof. The non-antifreeze protein, cell component or cell comprises a known protein, cell component or cell used in the pharmaceutical, medical, agricultural, veterinary and/or food industry(ies). The AFP protects the function of the non-antifreeze protein, microbe(s), cell component(s) or cell(s) under temperature stress (e.g., a temperature of −20-60° C.).