公开(公告)号：US3644177A

公开(公告)日：1972-02-22

申请号：US3644177D

申请日：1969-11-12

Applicant: YISSUM RES DEV CO

Inventor： ZYK NAOMI

IPC: C12Q1/34 ,  G01N31/22 ,  G01N31/14

CPC classification number: G01N31/22 ,  C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  Y10S435/805 ,  Y10S435/834 ,  Y10S435/836 ,  Y10S435/839 ,  Y10S435/883

Abstract: A method of determining the penicillin content in biological, metabolic, and alimentary substances by partially inactivating penicillinase with iodine in the presence of and in proportion to the amount of a penicillin contained in a specimen, and measuring the residual activity of the enzyme by contact with a suitable substrate such as an S-type penicillin in the presence of an indicator whose color change signifies accumulation of the hydrolyzate penicillinoic acid and is correlated in terms of time or hue to a standardized amount of the penicillin under test. Monitoring strips for carrying out the procedure consisting of 2 sorbefacient pads one of which is impregnated with the test enzyme while the other is suffused with an S-type penicillin and an indicator which may be a soluble starch-iodine complex or a pH-sensitive organic dye.