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    In vitro method for the simultaneous detection and identification of antibiotics of different classes and corresponding diagnostic kit
    1.
    发明授权
    In vitro method for the simultaneous detection and identification of antibiotics of different classes and corresponding diagnostic kit 有权
    用于同时检测和鉴定不同类别的抗生素的体外方法和相应的诊断试剂盒

    公开(公告)号：US07998754B2

    公开(公告)日：2011-08-16

    申请号：US11911375

    申请日：2006-04-13

    申请人： Benoit Granier

    发明人： Benoit Granier

    IPC分类号： G01N33/53

    CPC分类号： G01N33/9446 ,  G01N33/558 ,  G01N2415/00 ,  Y10S436/81

    摘要： The invention concerns a diagnostic kit for simultaneous assay of antibiotics of different classes. The kit contains a single reaction mixture containing at least one first labelled receptor, specific of recognition of β-lactams, a second labelled receptor, specifically and competitively identifying a tetracycline and a biotinylated nucleic acid fragment, an antisulfamide antibody and a labelled sulfamide analogue; and a recovery system in the form of a solid support comprising a nitrocellulose membrane whereon are fixed in three separate testing zones, respectively an antibiotic with β-lactam nucleus, an avidin and an antibody capable of specifically identifying the antisulfamide antibody, so that the labelling intensity detected on the recovery system at the three testing zones results independently from a competitive recognition of each antibiotic by its labelled receptor.

    摘要翻译： 本发明涉及用于同时测定不同类别的抗生素的诊断试剂盒。 试剂盒包含含有至少一种第一标记受体的单一反应混合物，特异性识别的β-内酰胺，第二标记受体，特异性和竞争性鉴定四环素和生物素化的核酸片段，反硫酰胺抗体和标记的磺酰胺类似物 ; 以及固体支持物形式的回收系统，其包含硝基纤维素膜，其固定在三个独立的测试区中，分别具有含有β-内酰胺核的抗生素，抗生物素蛋白和能够特异性鉴定抗血清酰胺抗体的抗体， 在三个测试区的回收系统上检测到的标记强度独立于其标记受体对每种抗生素的竞争性识别。

    Substrates for beta-lactamase and uses thereof
    2.
    发明申请
    Substrates for beta-lactamase and uses thereof 有权
    β-内酰胺酶底物及其用途

    公开(公告)号：US20070184513A1

    公开(公告)日：2007-08-09

    申请号：US11606642

    申请日：2006-11-29

    申请人： Roger Tsien ,  Gregor Zlokarnik

    发明人： Roger Tsien ,  Gregor Zlokarnik

    IPC分类号： C12Q1/18 ,  C07D501/02 ,  C07D501/14

    CPC分类号： C07D501/36 ,  C07D501/00 ,  C07D505/00 ,  C07D519/00 ,  C12N9/86 ,  C12N15/85 ,  C12N2800/90 ,  C12N2830/002 ,  C12P17/184 ,  C12P17/188 ,  C12P35/00 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/6897 ,  C12Q2334/00 ,  G01N2333/986 ,  G01N2415/00 ,  Y10S435/805

    摘要： Substrates for β-lactamase of the general formula I in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R′ is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH2)nOH, in which n is 0 or an integer from 1 to 5; R″ is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, CHR2OCO(CH2)nCH3, —CHR2OCOC (CH3)3, acylthiomethyl, acyloxy-alpha -benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO2 and CH2; and Z′ and Z″ are linkers for the fluorescent donor and quencher moieties. Methods of assaying β-lactamase activity and monitoring expression in systems using β-lactamase as a reporter gene also are disclosed.

    摘要翻译： 通式I的β-内酰胺酶底物，其中X和Y中的一个是荧光供体部分，另一个是猝灭剂（其可以或不再发射）; R'选自H，低级（即1至约5个碳原子的烷基）和（CH 2 CH 2）n OH，其中n是 0或1〜5的整数; R“选自H，生理学上可接受的金属和铵阳离子，CHR 2 OCO（CH 2）n CH 2 CH 2， （CH 3）3，酰基硫甲基，酰氧基-α-苄基，δ-丁内酰胺 ，甲氧基羰氧基甲基，苯基，甲基亚磺酰基甲基，β-吗啉代乙基，二烷基氨基乙基，酰氧基烷基，二烷基氨基羰氧基甲基和脂族基，其中R 2选自H和低级烷基; A选自S，O，SO，SO 2和CH 2; Z'和Z“是荧光供体和猝灭剂部分的接头。 公开了使用β-内酰胺酶作为报道基因的体内测定β-内酰胺酶活性和监​​测表达的方法。

    Probe for mass spectrometry
    3.
    发明申请
    Probe for mass spectrometry 审中-公开
    探测质谱

    公开(公告)号：US20050196791A1

    公开(公告)日：2005-09-08

    申请号：US11054050

    申请日：2005-02-08

    申请人： Jens-Oliver Koopman ,  Jonathan Blackburn

    发明人： Jens-Oliver Koopman ,  Jonathan Blackburn

    IPC分类号： G01N27/64 ,  A61K47/48 ,  C07K14/00 ,  G01N27/62 ,  G01N33/483 ,  G01N33/53 ,  G01N33/94 ,  G01N37/00 ,  H01J49/04 ,  H01J49/16 ,  C12Q1/68 ,  C12M1/34 ,  H01J49/00

    CPC分类号： H01J49/0418 ,  A61K47/6809 ,  B82Y30/00 ,  C07K14/00 ,  G01N33/9446 ,  G01N2415/00 ,  Y10T436/24

    摘要： The present invention relates to a probe for the analysis of one or more analytes, particularly proteins or compounds capable of binding or otherwise interacting therewith, by laser desorption/ionisation mass spectrometry, more particularly MALDI MS. It also relates to a protein microarray, a method of producing a protein microarray and a method of analysing a protein microarray. The probe comprises a support having an electroconductive target surface thereon characterized in that the target surface comprises a micro array having a plurality of discrete target areas presenting one or more analyte capture moieties. Each discrete target area has an area of less than 1000 μm2, more preferably still less than 500 μm2, and more preferably still less than 100 μm2.

    摘要翻译： 本发明涉及通过激光解吸/电离质谱法，更具体地说是MALDI MS分析一种或多种分析物，特别是能够结合或以其它方式相互作用的蛋白质或化合物的探针。 它还涉及蛋白质微阵列，产生蛋白质微阵列的方法和分析蛋白质微阵列的方法。 探针包括其上具有导电目标表面的支撑体，其特征在于，目标表面包括具有呈现一个或多个分析物捕获部分的多个离散靶区域的微阵列。 每个离散目标区域的面积小于1000μm2，更优选小于500μm2，更优选小于100μm2， / SUP>。

    Enzymatic process for the determination of beta-lactam antibiotics
    7.
    发明授权
    Enzymatic process for the determination of beta-lactam antibiotics 失效
    用于测定β-内酰胺抗生素的酶法

    公开(公告)号：US4546076A

    公开(公告)日：1985-10-08

    申请号：US462233

    申请日：1983-01-31

    申请人： Jacques Degelaen ,  Albert Loffet ,  Jean-Pierre Durieux

    发明人： Jacques Degelaen ,  Albert Loffet ,  Jean-Pierre Durieux

    IPC分类号： G01N33/50 ,  C12N9/52 ,  C12N11/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/37 ,  C12R1/03 ,  C12Q1/36

    CPC分类号： C12Q1/34 ,  C12N11/08 ,  G01N2415/00

    摘要： An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

    摘要翻译： 一种用于测定生物液体中β-内酰胺抗生素的酶促方法，包括以下步骤：（1）将该液体与由Actinomadura R 39产生的可溶性D-丙氨酰-D-丙氨酸 - 羧肽酶一起孵育，所述酶固定在 水不溶性载体，β-内酰胺抗生素与固定化酶反应形成无活性和等分子酶 - 抗生素复合物; （2）将固定化酶与液体分离并洗涤; （3）将固定化酶与底物溶液一起温育以使其水解并形成一定数量的对应于残留的酶活性的D-丙氨酸; （4）确定形成的D-丙氨酸的量; 和（5）将步骤（4）的测定与标准进行比较以获得生物液体中抗生素的浓度; 以及用于进行该方法并包含必要试剂的试验组。

    Reagent for the determination of .beta.-lactamase
    8.
    发明授权
    Reagent for the determination of .beta.-lactamase 失效
    用于测定β-内酰胺酶的试剂

    公开(公告)号：US4383032A

    公开(公告)日：1983-05-10

    申请号：US265267

    申请日：1981-05-20

    申请人： Peter Stahl ,  Wolfgang Vomel ,  Hans Seidel

    发明人： Peter Stahl ,  Wolfgang Vomel ,  Hans Seidel

    IPC分类号： G01N33/50 ,  C12Q1/06 ,  C12Q1/34 ,  C12Q1/04 ,  C12Q1/30 ,  C12Q1/38

    CPC分类号： C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  Y10S435/805 ,  Y10S435/81

    摘要： The present invention provides a process for the determination of .beta.-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen.The present invention also provides a reagent for the determination of .beta.-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.

    摘要翻译： 本发明提供了通过使用7-氰基乙酰氨基头孢烷酸测定β-内酰胺酶的方法和由此形成的着色材料的测量，其中铵离子与磷酸根离子和/或 药剂分解氧气。 本发明还提供了一种用于测定基于7-氰基乙酰氨基头孢烷酸的β-内酰胺酶的试剂，该试剂另外含有铵离子，以及磷酸根离子和/或分解氧的试剂。

    Substrates for beta-lactamase and uses thereof
    10.
    发明申请
    Substrates for beta-lactamase and uses thereof 有权
    β-内酰胺酶底物及其用途

    公开(公告)号：US20030119085A1

    公开(公告)日：2003-06-26

    申请号：US10280482

    申请日：2002-10-24

    申请人： The Regents of the University of California

    发明人： Roger Y. Tsien ,  Gregor Zlokarnik

    IPC分类号： C12Q001/18 ,  C07D55/16 ,  C07D51/14 ,  C07D487/04

    CPC分类号： C07D501/36 ,  C07D501/00 ,  C07D505/00 ,  C07D519/00 ,  C12N9/86 ,  C12N15/85 ,  C12N2800/90 ,  C12N2830/002 ,  C12P17/184 ,  C12P17/188 ,  C12P35/00 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/6897 ,  C12Q2334/00 ,  G01N2333/986 ,  G01N2415/00 ,  Y10S435/805

    摘要： Substrates for null-lactamase of the general formula I 1 in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); Rnull is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH2)nOH, in which n is 0 or an integer from 1 to 5; Rnull is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, nullCHR2OCO(CH2)nCH3, nullCHR2OCOC(CH3)3, acylthiomethyl, acyloxy-alpha-benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO2 and CH2; and Znull and Znull are linkers for the fluorescent donor and quencher moieties. Methods of assaying null-lactamase activity and monitoring expression in systems using null-lactamase as a reporter gene also are disclosed.

    摘要翻译： 通式I的β-内酰胺酶底物，其中X和Y中的一个是荧光供体部分，另一个是猝灭剂（其可以或不再发射）; R'选自H，低级（即1至约5个碳原子的烷基）和（CH 2）nOH，其中n为0或1至5的整数; R“选自H，生理学上可接受的金属和铵阳离子，-CHR2OCO（CH2）nCH3，-CHR2OCOC（CH3）3，酰基硫代甲基，酰氧基-α-苄基，δ-丁基内酰基，甲氧基羰氧基甲基，苯基，甲基亚磺酰基甲基 ，β-吗啉代乙基，二烷基氨基乙基，酰氧基烷基，二烷基氨基羰氧基甲基和脂族基，其中R2选自H和低级烷基; A选自S，O，SO，SO 2和CH 2; Z'和Z“是荧光供体和猝灭剂部分的接头。 公开了使用β-内酰胺酶作为报道基因的体内测定β-内酰胺酶活性和监​​测表达的方法。