公开(公告)号：US20180265929A1

公开(公告)日：2018-09-20

申请号：US15991725

申请日：2018-05-29

申请人： Yafeng Dong ,  Carl Weiner

发明人： Yafeng Dong ,  Carl Weiner

IPC分类号： C12Q1/6883 ,  C12Q1/6876

CPC分类号： C12Q1/6883 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N2800/368

摘要： A method of detecting a combination of nucleic acid biomarkers in a human subject can include: obtaining a nucleic acid sample from the human subject; selecting the combination of nucleic acid biomarkers; analyzing a transcriptome of the human subject for the combination of nucleic acid biomarkers in the nucleic acid sample from the human subject; detecting in the nucleic acid sample the presence of the combination of nucleic acid biomarkers, wherein each nucleic acid biomarker in the combination of nucleic acid biomarkers has a variation from a transcription standard.

公开(公告)号：US20130296190A1

公开(公告)日：2013-11-07

申请号：US13990495

申请日：2011-11-30

申请人： Yafeng Dong ,  Carl Weiner

发明人： Yafeng Dong ,  Carl Weiner

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N2800/368

摘要： A nucleic acid normalization kit can include a nucleic acid having a normalization sequence including or complementary to one or more of SEQ ID NOs: 1-4 and 301-303 or unique segment thereof, the nucleic acid being present in an amount sufficient for use in a nucleic acid normalization protocol. The normalization kit can be used in a method of identifying a pregnancy normalization nucleic acid sequence. A nucleic acid diagnostic kit for diagnosing susceptibility to preterm birth (PTB) can include a nucleic acid having a CFP RNA PTB biomarker sequence including or complementary to one or more of SEQ ID NOs: 5-300 or unique segment thereof, the nucleic acid being present in an amount sufficient for use in a nucleic acid diagnostic protocol for diagnosing susceptibility to PTB. The diagnostic kit can be used in a method for predicting susceptibility of a pregnant woman to preterm birth (PTB).

摘要翻译： 核酸标准化试剂盒可以包括具有包括或互补于SEQ ID NO：1-4和301-303中的一个或多个或其独特片段的归一化序列的核酸，所述核酸以足以用于 核酸标准化方案。 归一化试剂盒可用于鉴定怀孕归一化核酸序列的方法。 用于诊断早产易感性的核酸诊断试剂盒可以包括具有包含SEQ ID NO：5-300或其独特片段中的一个或多个的CFP RNA PTB生物标志物序列的核酸，其核酸是 存在足以用于诊断PTB易感性的核酸诊断方案中的量。 诊断试剂盒可用于预测孕妇对早产（PTB）的易感性的方法。

公开(公告)号：US20150376709A1

公开(公告)日：2015-12-31

申请号：US14851809

申请日：2015-09-11

申请人： Yafeng Dong ,  Carl Weiner

发明人： Yafeng Dong ,  Carl Weiner

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N2800/368

摘要： A nucleic acid normalization kit can include a nucleic acid having a normalization sequence including or complementary to one or more of SEQ ID NOs: 1-4 and 301-303 or unique segment thereof, the nucleic acid being present in an amount sufficient for use in a nucleic acid normalization protocol. The normalization kit can be used in a method of identifying a pregnancy normalization nucleic acid sequence. A nucleic acid diagnostic kit for diagnosing susceptibility to preterm birth (PTB) can include a nucleic acid having a CFP RNA PTB biomarker sequence including or complementary to one or more of SEQ ID NOs: 5-300 or unique segment thereof, the nucleic acid being present in an amount sufficient for use in a nucleic acid diagnostic protocol for diagnosing susceptibility to PTB. The diagnostic kit can be used in a method for predicting susceptibility of a pregnant woman to preterm birth (PTB).

摘要翻译： 核酸标准化试剂盒可以包括具有包括或互补于SEQ ID NO：1-4和301-303中的一个或多个或其独特片段的归一化序列的核酸，所述核酸以足以用于 核酸标准化方案。 归一化试剂盒可用于鉴定怀孕归一化核酸序列的方法。 用于诊断早产易感性的核酸诊断试剂盒可以包括具有包含或互补于SEQ ID NO：5-300或其独特片段的CFP RNA PTB生物标志物序列的核酸，所述核酸是 存在足以用于诊断PTB易感性的核酸诊断方案中的量。 诊断试剂盒可用于预测孕妇对早产（PTB）的易感性的方法。

公开(公告)号：US20210095347A1

公开(公告)日：2021-04-01

申请号：US17118322

申请日：2020-12-10

申请人： Yafeng Dong ,  Carl Weiner

发明人： Yafeng Dong ,  Carl Weiner

IPC分类号： C12Q1/6883 ,  C12Q1/6876

摘要： A method of detecting a combination of nucleic acid biomarkers in a human subject can include: obtaining a nucleic acid sample from the human subject; selecting the combination of nucleic acid biomarkers; analyzing a transcriptome of the human subject for the combination of nucleic acid biomarkers in the nucleic acid sample from the human subject; detecting in the nucleic acid sample the presence of the combination of nucleic acid biomarkers, wherein each nucleic acid biomarker in the combination of nucleic acid biomarkers has a variation from a transcription standard.

公开(公告)号：US10954564B2

公开(公告)日：2021-03-23

申请号：US15991725

申请日：2018-05-29

申请人： Yafeng Dong ,  Carl Weiner

发明人： Yafeng Dong ,  Carl Weiner

IPC分类号： C12Q1/6883 ,  C12Q1/6876

摘要： A method of detecting a combination of nucleic acid biomarkers in a human subject can include: obtaining a nucleic acid sample from the human subject; selecting the combination of nucleic acid biomarkers; analyzing a transcriptome of the human subject for the combination of nucleic acid biomarkers in the nucleic acid sample from the human subject; detecting in the nucleic acid sample the presence of the combination of nucleic acid biomarkers, wherein each nucleic acid biomarker in the combination of nucleic acid biomarkers has a variation from a transcription standard.

公开(公告)号：US20210199673A1

公开(公告)日：2021-07-01

申请号：US17203534

申请日：2021-03-16

申请人： Carl Philip Weiner ,  Yafeng Dong

发明人： Carl Philip Weiner ,  Yafeng Dong

IPC分类号： G01N33/68 ,  G01N33/53

摘要： Methods for detecting a Group of Biomarkers is provided herein. The translation profile of the Group of Biomarkers can be used for determining whether a subject, such as a fetus, has Down syndrome The methods include detecting one or more specific groups of biomarkers in a biological sample, and determining whether the expression of the biomarkers is altered when compared to expression of the biomarkers in one or more subjects that do not have trisomy 21 (e.g., a transcriptional standard). The biological sample can be a blood sample, and the biomarkers are cell free plasma RNAs.