公开(公告)号：US20090181413A1

公开(公告)日：2009-07-16

申请号：US12088321

申请日：2006-09-29

申请人： Yasuki Itoh ,  Keiko Matsumoto ,  Hiroshi Matsui ,  Yuhko Hirao

发明人： Yasuki Itoh ,  Keiko Matsumoto ,  Hiroshi Matsui ,  Yuhko Hirao

IPC分类号： C12Q1/60 ,  G01N21/00 ,  C12Q1/50 ,  C12Q1/52 ,  C12Q1/32 ,  C12Q1/37 ,  G01N33/92 ,  C12Q1/28

CPC分类号： G01N21/78 ,  C12Q1/60 ,  C12Q1/61 ,  G01N21/272 ,  G01N33/92

摘要： This invention provides a method for differentially and simultaneously quantifying two target analytes via a single assay operation with the use of a single type of reagent in each of the two steps, i.e., the first step and the second step. In this method, the reaction product associated with the first target analyte is detected at an early stage of the second step and the reaction product associated with the second target analyte is then detected.

摘要翻译： 本发明提供了一种用于通过使用单个类型的试剂在两个步骤中的每一个，即第一步骤和第二步骤中通过单一测定操作来差异和同时定量两种目标分析物的方法。 在该方法中，在第二步骤的早期阶段检测与第一目标分析物相关的反应产物，然后检测与第二目标分析物相关的反应产物。

公开(公告)号：US20130230873A1

公开(公告)日：2013-09-05

申请号：US13884475

申请日：2011-11-09

申请人： Yuhko Hirao

发明人： Yuhko Hirao

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60 ,  C12Q1/44 ,  G01N33/92 ,  G01N2333/918

摘要： A method for quantifying remnant-like lipoprotein cholesterol in a sample simply and accurately without requiring separation operations, and a kit therefor are disclosed. A method for quantifying cholesterol in a remnant-like lipoprotein in a sample containing different lipoproteins including the remnant-like lipoprotein comprises a step (1) of erasing cholesterol in lipoproteins other than the remnant-like lipoprotein; and a step (2) of quantifying cholesterol in the remaining remnant-like lipoprotein. The step (1) is carried out under an action of a cholesterol esterase having a molecular weight of more than 40 kDa and not having a subunit having a molecular weight of not more than 40 kDa; and the step (2) is carried out under an action of a cholesterol esterase having a molecular weight of not more than 40 kDa or a cholesterol esterase having a subunit having a molecular weight of not more than 40 kDa.

摘要翻译： 公开了一种用于在不需要分离操作的情况下简单准确地量化样品中的残留样脂蛋白胆固醇的方法，以及用于其的试剂盒。 在含有残留样脂蛋白的不同脂蛋白的样品中，用于量化残留样脂蛋白中的胆固醇的方法包括除残留样脂蛋白以外的脂蛋白中的胆固醇消除的步骤（1） 以及量化残留的类似脂蛋白中的胆固醇的步骤（2）。 步骤（1）在分子量大于40kDa且不具有分子量不超过40kDa的亚单位的胆固醇酯酶的作用下进行; 并且步骤（2）在分子量不超过40kDa的胆固醇酯酶或具有分子量不超过40kDa的亚单位的胆固醇酯酶的作用下进行。

公开(公告)号：US20110171672A1

公开(公告)日：2011-07-14

申请号：US12677676

申请日：2008-09-11

申请人： Yuhko Hirao

发明人： Yuhko Hirao

IPC分类号： C12Q1/30 ,  C12Q1/60

CPC分类号： C12Q1/30

摘要： Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.

摘要翻译： 本发明公开了一种用于定量待测组分的方法中由叠氮化物抑制过氧化氢酶的测量误差的方法，其中来源于待测组分的成分的过氧化氢通过过氧化氢酶分解。 减少由叠氮化物对过氧化氢酶抑制的测量误差的方法采用过氧化氢酶，其具有分子量为75kDa以上的亚基，并且来源于微生物，当来自除被测定成分以外的成分的过氧化氢 通过过氧化氢酶分解，然后定量来自待测组分的过氧化氢，以量化待测组分。

公开(公告)号：US10415077B2

公开(公告)日：2019-09-17

申请号：US12677676

申请日：2008-09-11

申请人： Yuhko Hirao

发明人： Yuhko Hirao

IPC分类号： C12Q1/30

摘要： Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.

公开(公告)号：US08906638B2

公开(公告)日：2014-12-09

申请号：US13884475

申请日：2011-11-09

申请人： Yuhko Hirao

发明人： Yuhko Hirao

IPC分类号： C12Q1/60 ,  G01N33/92 ,  C12Q1/44

CPC分类号： C12Q1/60 ,  C12Q1/44 ,  G01N33/92 ,  G01N2333/918

摘要： A method for quantifying remnant-like lipoprotein cholesterol in a sample simply and accurately without requiring separation operations, and a kit therefor are disclosed. A method for quantifying cholesterol in a remnant-like lipoprotein in a sample containing different lipoproteins including the remnant-like lipoprotein comprises a step (1) of erasing cholesterol in lipoproteins other than the remnant-like lipoprotein; and a step (2) of quantifying cholesterol in the remaining remnant-like lipoprotein. The step (1) is carried out under an action of a cholesterol esterase having a molecular weight of more than 40 kDa and not having a subunit having a molecular weight of not more than 40 kDa; and the step (2) is carried out under an action of a cholesterol esterase having a molecular weight of not more than 40 kDa or a cholesterol esterase having a subunit having a molecular weight of not more than 40 kDa.

摘要翻译： 公开了一种用于在不需要分离操作的情况下简单准确地量化样品中的残留样脂蛋白胆固醇的方法，以及用于其的试剂盒。 在含有残留样脂蛋白的不同脂蛋白的样品中，用于定量残留样脂蛋白中的胆固醇的方法包括除残留样脂蛋白以外的脂蛋白中除去胆固醇的步骤（1） 以及量化残留的类似脂蛋白中的胆固醇的步骤（2）。 步骤（1）在分子量大于40kDa且不具有分子量不超过40kDa的亚单位的胆固醇酯酶的作用下进行; 并且步骤（2）在分子量不超过40kDa的胆固醇酯酶或具有分子量不超过40kDa的亚单位的胆固醇酯酶的作用下进行。