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公开(公告)号：US5514551A
公开(公告)日：1996-05-07
申请号：US323257
申请日：1994-10-14
申请人： Yeasing Yang , Paul D. Stull , Marc Spingola
发明人： Yeasing Yang , Paul D. Stull , Marc Spingola
CPC分类号： C12Q1/689
摘要： Oligonucleotides and methods for the amplification and specific detection of Chlamydia trachomatis. The invention relates to amplification oligonucleotides capable of amplifying Chlamydia trachomatis nucleotide sequences and to probes and helper oligonucleotides for the specific detection of Chlamydia trachomatis nucleic acids. The invention also relates to methods for using the oligonucleotides of the present invention and specific combinations and kits useful for the detection of Chlamydia trachomatis.
摘要翻译： 寡核苷酸和扩增和特异性检测沙眼衣原体的方法。 本发明涉及能够扩增沙眼衣原体核苷酸序列的扩增寡核苷酸和用于特异性检测沙眼衣原体核酸的探针和辅助寡核苷酸的扩增寡核苷酸。 本发明还涉及使用本发明的寡核苷酸的方法和用于检测沙眼衣原体的特异性组合和试剂盒。
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公开(公告)号：US5512445A
公开(公告)日：1996-04-30
申请号：US450186
申请日：1995-05-25
申请人： Yeasing Yang , Paul D. Stull , Marc Spingola
发明人： Yeasing Yang , Paul D. Stull , Marc Spingola
CPC分类号： C12Q1/689
摘要： Oligonucleotides and methods for the amplification and specific detection of Chlamydia trachomatis. The invention relates to amplification oligonucleotides capable of amplifying Chlamydia trachomatis nucleotide sequences and to probes and helper oligonucleotides for the specific detection of Chlamydia trachomatis nucleic acids. The invention also relates to methods for using the oligonucleotides of the present invention and specific combinations and kits useful for the detection of Chlamydia trachomatis.
摘要翻译： 寡核苷酸和扩增和特异性检测沙眼衣原体的方法。 本发明涉及能够扩增沙眼衣原体核苷酸序列的扩增寡核苷酸和用于特异性检测沙眼衣原体核酸的探针和辅助寡核苷酸的扩增寡核苷酸。 本发明还涉及使用本发明的寡核苷酸的方法和用于检测沙眼衣原体的特异性组合和试剂盒。
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公开(公告)号：US6087133A
公开(公告)日：2000-07-11
申请号：US972799
申请日：1997-11-18
CPC分类号： C12Q1/6844
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切核酸酶活性。
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公开(公告)号：USRE39007E1
公开(公告)日：2006-03-07
申请号：US10164833
申请日：2002-06-07
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonu- clease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切酶活性。
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公开(公告)号：USRE38960E1
公开(公告)日：2006-01-31
申请号：US10165281
申请日：2002-06-07
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
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公开(公告)号：US06214587B1
公开(公告)日：2001-04-10
申请号：US09506282
申请日：2000-02-17
IPC分类号： C12P1934
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切核酸酶活性。
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