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公开(公告)号：US6087133A
公开(公告)日：2000-07-11
申请号：US972799
申请日：1997-11-18
CPC分类号： C12Q1/6844
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切核酸酶活性。
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公开(公告)号：USRE39007E1
公开(公告)日：2006-03-07
申请号：US10164833
申请日：2002-06-07
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonu- clease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切酶活性。
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公开(公告)号：USRE38960E1
公开(公告)日：2006-01-31
申请号：US10165281
申请日：2002-06-07
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
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公开(公告)号：US06214587B1
公开(公告)日：2001-04-10
申请号：US09506282
申请日：2000-02-17
IPC分类号： C12P1934
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切核酸酶活性。
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5.发明授权
公开(公告)号：US5916777A
公开(公告)日：1999-06-29
申请号：US949076
申请日：1997-10-10
摘要： The present invention relates to methods of enzymatically synthesizing oligonucleotides. In particular, it relates to the use of 3'-ribonucleotide primers which effectively serve to initiate oligonucleotide synthesis, and can also be easily cleaved from synthesis products and reused in subsequent synthesis reactions.
摘要翻译： 本发明涉及酶促合成寡核苷酸的方法。 特别涉及使用有效用于引发寡核苷酸合成的3'-核糖核苷酸引物，也可以容易地从合成产物中切割出来，并在随后的合成反应中重复使用。
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6.发明授权Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides 失效限制性内切核酸酶序列用于切割硫代磷酸酯寡核苷酸
公开(公告)号：US5652126A
公开(公告)日：1997-07-29
申请号：US484816
申请日：1995-06-07
摘要： The present invention relates to methods of cleaving phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences can be used to facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.
摘要翻译： 本发明涉及切割硫代磷酸酯寡核苷酸的方法。 特别地，它涉及使用某些限制性内切酶来切割含有限制性内切核酸酶识别序列的硫代磷酸酯寡核苷酸。 这些限制性序列可用于促进相对切割抗性的硫代磷酸酯寡核苷酸的切割，从而促进其在合成后的分离和纯化。
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7.发明授权Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases 失效使用限制性内切酶酶解合成硫代磷酸酯寡核苷酸
公开(公告)号：US5739311A
公开(公告)日：1998-04-14
申请号：US476625
申请日：1995-06-07
摘要： The present invention relates to methods of synthesizing phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.
摘要翻译： 本发明涉及合成硫代磷酸酯寡核苷酸的方法。 特别地，它涉及使用某些限制性内切酶来切割含有限制性内切核酸酶识别序列的硫代磷酸酯寡核苷酸。 这些限制性序列有助于相对切割的硫代磷酸酯寡核苷酸的切割，从而促进它们在合成后的分离和纯化。
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8.发明申请Compositions and methods for detecting pathogenic bacteria expressing chaperonin proteins 审中-公开用于检测表达伴侣蛋白的致病菌的组合物和方法公开(公告)号：US20060073475A1
公开(公告)日：2006-04-06
申请号：US10216488
申请日：2002-08-09
申请人： Nanibhushan Dattagupta , Ketan Shah
发明人： Nanibhushan Dattagupta , Ketan Shah
CPC分类号： C12Q1/689 , C12Q1/6837
摘要： This invention relates generally to compositions and methods for detection of pathogenic bacteria that express extracellular chaperonin proteins. In one embodiment, it relates to Mycobacterium tuberculosis (Mtb) detection and provides for novel probes for a specific and sensitive diagnostic test for Mycobacterium tuberculosis complex (TBC) that hybridize with the groEL-1 gene. Arrays comprising the novel probes immobilized on a support for hybridization analysis and methods for TBC detection using the probes are also provided.
摘要翻译： 本发明一般涉及用于检测表达细胞外伴侣蛋白的致病菌的组合物和方法。 在一个实施方案中，其涉及结核分枝杆菌（Mtb）检测，并提供用于与groEL-1基因杂交的结核分枝杆菌复合物（TBC）的特异性和灵敏的诊断测试的新型探针。 还提供了包含固定在用于杂交分析的载体上的新型探针的阵列和使用探针进行TBC检测的方法。
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9.发明授权公开(公告)号：US07009041B1
公开(公告)日：2006-03-07
申请号：US08480472
申请日：1995-06-06
申请人： Sherrol H. McDonough , Daniel L. Kacian , Nanibhushan Dattagupta , Diane L. McAllister , Philip W. Hammond , Thomas B. Ryder
发明人： Sherrol H. McDonough , Daniel L. Kacian , Nanibhushan Dattagupta , Diane L. McAllister , Philip W. Hammond , Thomas B. Ryder
摘要： A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
摘要翻译： 提供了一种用于在基本上恒定温度，离子强度和pH条件下自动催化靶核酸序列的多个拷贝的方法，组合物和试剂盒，其中靶序列的多个RNA拷贝自动催化产生另外的拷贝，使用封闭和 未封闭的引物和/或启动子 - 引物以引发DNA和RNA合成，优选地减少非特异性产物形成。 本发明可用于产生核酸靶序列的拷贝，目的包括在临床，环境，法医学和相似样品中定量特异性核酸序列的测定，克隆和产生探针。
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10.发明授权Method for the intracellular delivery of biomolecules using thiocationic lipids 失效使用硫代阳离子脂质细胞内转运生物分子的方法
公开(公告)号：US5759519A
公开(公告)日：1998-06-02
申请号：US482430
申请日：1995-06-07
CPC分类号： C12N15/88 , A61K47/48023 , A61K47/48046 , A61K9/1272 , C07C381/12
摘要： Lipid molecules bearing a cationic charge are described. These cationic lipids are useful in the delivery of biomolecules, such as oligonucleotides, nucleic acids, peptides, diagnostic imaging agents, proteins and drug molecules. In the form of liposomes, they can effectively be used for the intracellular delivery of biomolecules for therapeutic or diagnostic purposes.
摘要翻译： 描述了带有阳离子电荷的脂质分子。 这些阳离子脂质可用于递送生物分子，如寡核苷酸，核酸，肽，诊断成像剂，蛋白质和药物分子。 以脂质体的形式，它们可以有效地用于生物分子的细胞内递送用于治疗或诊断目的。
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