公开(公告)号：US20230132790A1

公开(公告)日：2023-05-04

申请号：US17915500

申请日：2021-03-29

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Anthony H. FUTERMAN ,  Israel SILMAN ,  Joel L. SUSSMAN ,  Sarel FLEISHMAN ,  Adi GOLDENZWEIG ,  Sarka POKORNA ,  Yaacov ASHANI ,  Olga KHERSONSKY

IPC: C12N9/24

Abstract: A genetically modified human beta-glucocerebrosidase (GCase) is disclosed. The genetically modified GCase comprising an amino acid sequence at least 85% identical to SEQ ID NO: 2; and comprising mutations at coordinates L34P, K224N/G, T369E and N370D, where the coordinates correspond to said SEQ ID NO: 2; and capable of catalyzing hydrolysis of a glycolipid glucosylceramide (GlcCer). Pharmaceutical compositions comprising the genetically modified GCase and therapeutic methods of using same are also disclosed.

公开(公告)号：US20200058367A1

公开(公告)日：2020-02-20

申请号：US16672601

申请日：2019-11-04

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Sarel FLEISHMAN ,  Adi GOLDENZWEIG ,  Dan S. TAWFIK

IPC: G16B15/00 ,  G16B15/20 ,  G16C20/60 ,  G16C20/10 ,  G16B35/00 ,  G16B30/00 ,  C12N9/10 ,  C12N9/16 ,  C12N9/18

Abstract: A method for designing and selecting a protein having a stabilized structure compared to a corresponding wild type protein, and proteins having at least six amino acid substitutions with respect to a corresponding wild type protein, designed for improved thermal stability, improved specific activity and/or improved expression levels, are provided herein.