公开(公告)号：US20190083836A1

公开(公告)日：2019-03-21

申请号：US15535050

申请日：2015-12-10

Applicant: Yeda Research and Development Co, Ltd ,  University of Washington

Inventor： Dan S. TAWFIK ,  Haim LEADER ,  Yaacov ASHANI ,  Izhack CHERNY ,  Moshe GOLDSMITH ,  Per Jr. GRIESEN ,  Sagar D KHARE ,  Gustav Oberdorfer ,  David Baker ,  Sarel Fleishman ,  Adi Goldenzweig ,  Nidhi Aggarwal

IPC: A62D3/02 ,  C12N9/16 ,  A61P25/00

CPC classification number: A62D3/02 ,  A61P25/00 ,  A62D2101/02 ,  A62D2101/26 ,  C12N9/16 ,  C12Y301/08001

Abstract: A genetically modified polypeptide is disclosed which comprises an amino acid sequence of phosphotriesterase (PTE) having at least twice the catalytic efficiency for a V-type nerve agent as a polypeptide which consists of the sequence as set forth in SEQ ID NO: 1, when assayed under identical conditions.

公开(公告)号：US20210178207A1

公开(公告)日：2021-06-17

申请号：US17267816

申请日：2019-08-14

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Sarel FLEISHMAN ,  Dan S. TAWFIK ,  Olga KHERSONSKY

IPC: A62D3/02

Abstract: Provided herein is a library of designed phosphotriesterase (PTE) enzymes, exhibiting an improved catalytic hydrolysis activity of various substrates, including nerve agents, and a general method of generating and using the same.

公开(公告)号：US20200058367A1

公开(公告)日：2020-02-20

申请号：US16672601

申请日：2019-11-04

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Sarel FLEISHMAN ,  Adi GOLDENZWEIG ,  Dan S. TAWFIK

IPC: G16B15/00 ,  G16B15/20 ,  G16C20/60 ,  G16C20/10 ,  G16B35/00 ,  G16B30/00 ,  C12N9/10 ,  C12N9/16 ,  C12N9/18

Abstract: A method for designing and selecting a protein having a stabilized structure compared to a corresponding wild type protein, and proteins having at least six amino acid substitutions with respect to a corresponding wild type protein, designed for improved thermal stability, improved specific activity and/or improved expression levels, are provided herein.

公开(公告)号：US20140079682A1

公开(公告)日：2014-03-20

申请号：US14058334

申请日：2013-10-21

Applicant: Yeda Research and Development Co., Ltd.

Inventor： Dan S. TAWFIK ,  Amir Aharoni ,  Leonid Gaydukov ,  Joel L. Sussman ,  Israel Silman

IPC: C12N9/18

CPC classification number: C12N9/18 ,  A61K38/00 ,  C12N9/16 ,  Y02A50/473

Abstract: Isolated polynucleotides and polypeptides encoded therefrom are provided. These include mutated PON enzymes with increased, modified or substantially the same substrate specificity as compared to respective wild-type PON. Also provided are kits and methods using these enzymes.

Abstract translation: 提供了分离的多核苷酸及其编码的多肽。 这些包括与相应的野生型PON相比具有增加的，修饰的或基本上相同的底物特异性的突变的PON酶。 还提供了使用这些酶的试剂盒和方法。

公开(公告)号：US20180223302A1

公开(公告)日：2018-08-09

申请号：US15737558

申请日：2016-06-22

Applicant: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. ,  Yeda Research and Development Co. Ltd., at the Weizmann Institute of Science

Inventor： Arren BAR-EVEN ,  Tobias ERB ,  Steffen LINDNER ,  Philippe MARLIERE ,  Dan S. TAWFIK

IPC: C12N15/82

CPC classification number: C12N15/8269 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention relates to an organism, a tissue, a cell or an organelle expressing enzymes which allow the conversion of 2-phosphoglycolate (2-PG; also known as glycolate 2-phosphate) into an intermediate compound of the Calvin-Benson-Bassham Cycle (CBBC) without releasing CO2. The organism, tissue, cell or organelle of the invention may be genetically engineered, transgenic and/or transplastomic so as to express at least one enzyme which is involved in this conversion. The present invention further relates to an organism, tissue, cell or organelle which comprises/expresses at least one enzyme which is involved in this conversion. The present invention further relates to a method for producing an organism, tissue, cell or organelle of the invention. The present invention further relates to a method of enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2. The present invention further relates to the use of an organism, tissue, cell or organelle of the invention for enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2.