公开(公告)号：US11162960B2

公开(公告)日：2021-11-02

申请号：US16437021

申请日：2019-06-11

申请人： Yi Xiao ,  Haixiang Yu ,  Juan Canoura ,  Yingzhu Liu

发明人： Yi Xiao ,  Haixiang Yu ,  Juan Canoura ,  Yingzhu Liu

IPC分类号： G01N33/94 ,  C12N15/115 ,  G01N33/58 ,  C12Q1/6811

摘要： The subject invention provides methods, assays, and products for detecting small-molecule targets in a complex sample in both clinical and field settings. The subject invention provides aptamer-based sensors and methods of use thereof. The subject invention provides exonuclease-based methods for generating structure-switching aptamers from fully folded or pre-folded aptamers and developing aptamer-based sensors for small-molecule detection. The method for detecting one or more small-molecule targets in a sample comprises contacting the sample with one or more aptamer-based sensor selective for each of the small-molecule targets, and detecting the small-molecule target in the sample.

公开(公告)号：US11796499B2

公开(公告)日：2023-10-24

申请号：US18065118

申请日：2022-12-13

申请人： Yi Xiao ,  Yingzhu Liu ,  Juan Canoura

发明人： Yi Xiao ,  Yingzhu Liu ,  Juan Canoura

IPC分类号： G01N27/327 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N27/3276 ,  G01N27/3277 ,  G01N33/5308 ,  G01N33/5438

摘要： The subject invention provides methods for fabricating electrochemical aptamer-based (E-AB) sensors with enhanced sensitivity, signal-to-noise ratios, LOD, and improved stability and reproducibility. The subject invention also provides methods for aptamer immobilization on the surface of the electrode, which favors sufficient spacing between aptamers at the microscale to achieve optimal target recognition, folding, and signal transduction. The E-AB sensors of the subject invention provide superior sensing regardless of the sequence or structure of the bound aptamers or the physiochemical properties of the target.

公开(公告)号：US11060095B2

公开(公告)日：2021-07-13

申请号：US17117317

申请日：2020-12-10

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/11 ,  C12N15/115 ,  A61K31/167 ,  A61K31/137 ,  G01N27/26

摘要： The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US10907162B2

公开(公告)日：2021-02-02

申请号：US16791101

申请日：2020-02-14

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/11 ,  C12N15/115 ,  G01N27/26 ,  A61K31/137 ,  A61K31/167

摘要： The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US10655132B1

公开(公告)日：2020-05-19

申请号：US16174764

申请日：2018-10-30

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/115 ,  G01N27/26 ,  A61K31/137 ,  A61K31/167

摘要： The subject invention provides a novel SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each structurally-similar molecule in said family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US11408850B2

公开(公告)日：2022-08-09

申请号：US17354342

申请日：2021-06-22

申请人： Yi Xiao ,  Juan Canoura

发明人： Yi Xiao ,  Juan Canoura

IPC分类号： C12N15/115 ,  G01N27/327 ,  G01N21/64

摘要： The subject invention provides materials and methods for single-step fluorescence and electrochemical detection of small molecules, e.g., fentanyl and its analogs, in a sample. The subjection invention provides nucleic acids materials, e.g., aptamers (nucleic acid oligonucleotides) that can bind to fentanyl and its analogs with nanomolar affinity and high specificity against illicit drugs, adulterants, and cutting agents commonly existing in seized samples. The method for detecting fentanyl and/or its analogs in a sample comprises contacting the sample with an aptamer-based sensor selective for fentanyl and its analogs, and sensitively, specifically, and rapidly detecting fentanyl and/or its analogs in the sample.

公开(公告)号：US11579110B2

公开(公告)日：2023-02-14

申请号：US17826891

申请日：2022-05-27

申请人： Yi Xiao ,  Juan Canoura

发明人： Yi Xiao ,  Juan Canoura

IPC分类号： C12N15/115 ,  G01N27/327 ,  G01N21/64

摘要： The subject invention provides materials and methods for single-step fluorescence and electrochemical detection of small molecules, e.g., fentanyl and its analogs, in a sample. The subjection invention provides nucleic acids materials, e.g., aptamers (nucleic acid oligonucleotides) that can bind to fentanyl and its analogs with nanomolar affinity and high specificity against illicit drugs, adulterants, and cutting agents commonly existing in seized samples. The method for detecting fentanyl and/or its analogs in a sample comprises contacting the sample with an aptamer-based sensor selective for fentanyl and its analogs, and sensitively, specifically, and rapidly detecting fentanyl and/or its analogs in the sample.

公开(公告)号：US10948504B2

公开(公告)日：2021-03-16

申请号：US16902717

申请日：2020-06-16

申请人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

发明人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

IPC分类号： C12N15/115 ,  C12Q1/6883 ,  G01N33/94 ,  G01N33/52

摘要： The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.

公开(公告)号：US10000790B2

公开(公告)日：2018-06-19

申请号：US14752970

申请日：2015-06-28

申请人： Pingping Liang ,  Haixiang Yu ,  Yi Xiao

发明人： Pingping Liang ,  Haixiang Yu ,  Yi Xiao

IPC分类号： C12Q1/32

CPC分类号： C12Q1/32

摘要： The subject invention provides materials and methods for detecting the presence of an electron donor. In a specific embodiment, the device detects the presence of dihydronicotinamide adenine dinucleotide (NADH) via a colorimetric change output by the sensing device. In another specific embodiment, the presence of an enzyme capable of catalyzing, or an agent capable of inhibiting, the production of NADH can also be detected by a colorimetric readout using the same device. In some embodiments, dihydronicotinamide adenine dinucleotide phosphate (NADPH) can also be detected using the device provided herein. Advantageously, preferred embodiments of the subject invention provide a low-cost, sensitive device for monitoring the presence of critical biological analytes in a variety of applications.

公开(公告)号：US09804178B2

公开(公告)日：2017-10-31

申请号：US14930242

申请日：2015-11-02

申请人： Daniel Roncancio ,  Haixiang Yu ,  Xiaowen Xu ,  Yi Xiao

发明人： Daniel Roncancio ,  Haixiang Yu ,  Xiaowen Xu ,  Yi Xiao

IPC分类号： C07H21/02 ,  C07H21/04 ,  G01N33/94 ,  C12N15/115 ,  A61K31/70

CPC分类号： G01N33/946 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10

摘要： The invention pertains to a rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements based on an aptamer sensor that reports the presence of cocaine via the displacement and unquenching of a bound fluorophore molecule. In certain embodiments, the invention provides novel aptamers, which have reduced background fluorescence, bind a fluorophore molecule tightly, and show an increased signal gain in the presence of cocaine.