PLANT VIRAL EXPRESSION VECTORS AND USE OF SAME FOR GENERATING GENOTYPIC VARIATIONS IN PLANT GENOMES
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    发明申请
    PLANT VIRAL EXPRESSION VECTORS AND USE OF SAME FOR GENERATING GENOTYPIC VARIATIONS IN PLANT GENOMES 审中-公开
    植物病毒表达载体及其在植物基因组中产生遗传变异的用途

    公开(公告)号:US20140331360A1

    公开(公告)日:2014-11-06

    申请号:US14341999

    申请日:2014-07-28

    IPC分类号: C12N15/82

    摘要: A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, thereby generating genotypic variation in the genome of the plant.

    摘要翻译: 公开了在植物基因组中产生基因型变异的方法。 该方法包括将至少一种编码至少一种嵌合核酸酶的病毒表达载体引入植物中,所述嵌合核酸酶包含DNA结合结构域,核酸酶和对含DNA细胞器的定位信号,其中所述DNA结合结构域介导核酸酶的特异性靶向 到植物的基因组,从而在植物的基因组中产生基因型变异。