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1.发明申请公开(公告)号：US20050095680A1
公开(公告)日：2005-05-05
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
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2.发明授权Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法公开(公告)号：US07297521B2
公开(公告)日：2007-11-20
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加， 或反转一个或几个氨基酸，并可构成 具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。
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3.发明授权Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法公开(公告)号：US06881566B2
公开(公告)日：2005-04-19
申请号：US09836470
申请日：2001-04-18
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽 序列至少包含氨基酸编号55至11 包括序列表中所示的SEQ ID NO：3的13个氨基酸残基，其包含一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基 具有SEQ ID NO：2中氨基酸序列号50至393的氨基酸序列。
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4.发明授权Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 失效棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法公开(公告)号：US06908754B2
公开(公告)日：2005-06-21
申请号：US10284334
申请日：2002-10-31
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加， 或反转一个或几个氨基酸，并可构成 具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。
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5.发明授权Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法公开(公告)号：US06255086B1
公开(公告)日：2001-07-03
申请号：US09629616
申请日：2000-07-31
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12P1310
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段，以及以下（c）或（d）:( a）定义的多肽，其至少具有氨基酸的氨基酸序列 序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽 序列至少包含氨基酸序号55至1113 o f序列号3所示，包括一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基的蛋白质， SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列。
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公开(公告)号：US20060057118A1
公开(公告)日：2006-03-16
申请号：US11210756
申请日：2005-08-25
CPC分类号： C12N9/0053 , A23K10/18 , A23K50/10 , C12N9/0044
摘要： A feed composition for ruminants comprising a microbe having nitrite reductase activity, wherein said microbe has been modified so that intracellular nitrite reductase activity is enhanced is disclosed. A feed composition for ruminants comprising a microbe having nitrite reductase activity, wherein said microbe is selected from the group consisting of intestinal bacteria, coryneform bacteria, Bacillus subtilis, bacteria of the genus Methylophilus, Actinomyces, ruminal bacteria, and a combination thereof is disclosed. A method of improving the growth of a ruminant by administering the feed composition as described above to said ruminant and a method of preventing nitrite intoxication by administering the feed composition as described above to a ruminant are disclosed.
摘要翻译： 一种用于反刍动物的饲料组合物，其包含具有亚硝酸还原酶活性的微生物，其中所述微生物已被修饰，使得细胞内亚硝酸还原酶活性增强。 一种用于反刍动物的饲料组合物，其包含具有亚硝酸还原酶活性的微生物，其中所述微生物选自肠细菌，棒状细菌，枯草芽孢杆菌，Methylophilus属细菌，放线菌属，瘤细菌及其组合。 公开了通过向所述反刍动物施用如上所述的饲料组合物来提高反刍动物生长的方法以及通过将上述饲料组合物给予反刍动物来预防亚硝酸盐中毒的方法。
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