公开(公告)号：US06599713B1

公开(公告)日：2003-07-29

申请号：US09913161

申请日：2001-08-10

申请人： Yoshihiro Hatanaka ,  Ryo Serizawa

发明人： Yoshihiro Hatanaka ,  Ryo Serizawa

IPC分类号： C12Q128

CPC分类号： C12Q1/28 ,  G01N33/5094 ,  G01N33/54393 ,  G01N33/56972 ,  G01N33/573 ,  G01N2333/902 ,  G01N2333/908

摘要： Disclosed is a method for determining a white blood cell count of a whole blood sample, which comprises: mixing a whole blood sample with a surfactant to thereby obtain a mixture; allowing the mixture to stand for a time sufficient to lyze the white blood cells contained in the whole blood sample and release intrinsic myeloperoxidase from the white blood cells; measuring the concentration of the released myeloperoxidase in the mixture; and determining the white blood cell count in the whole blood sample, based on the concentration of the released myeloperoxidase. Also disclosed is a method of the present invention, in which, in addition to a white blood cell count of a whole blood sample, the concentration of C-reactive protein contained in the same whole blood sample is measured.

摘要翻译： 公开了一种测定全血样品的白细胞计数的方法，其特征在于：将全血样品与表面活性剂混合，得到混合物; 使混合物静置足以裂解全血样品中所含的白细胞并从白细胞中释放本征髓过氧化物酶; 测量混合物中释放的髓过氧化物酶的浓度; 并根据所释放的髓过氧化物酶的浓度确定全血样品中的白细胞计数。 还公开了本发明的方法，其中，除了全血样品的白细胞计数之外，还测定同一全血样品中所含的C-反应蛋白的浓度。