公开(公告)号：US06203982B1

公开(公告)日：2001-03-20

申请号：US09068880

申请日：1998-09-02

申请人： Youichi Nunokawa ,  Shinzo Oikawa ,  Shoji Tanaka

发明人： Youichi Nunokawa ,  Shinzo Oikawa ,  Shoji Tanaka

IPC分类号： A61K3821

CPC分类号： C07D295/185 ,  A61K31/12 ,  A61K31/165 ,  A61K31/215 ,  C07C50/06 ,  C07C50/30 ,  C12N9/0075 ,  C12Q1/6897 ,  G01N2500/04

摘要： A plasmid was constructed, in which the hiNOS structural gene was replaced with the luciferase structural gene as a reporter gene, with retaining functions of the hiNOS gene 5′-promoter region and 3′-untranslated region. This plasmid was stably transfected into human cell lines. The above transformed cells selectively expressed the reporter gene in the presence of inducers. It has become possible, by examining the reporter gene expression in these transformed cells, to simply and easily screen, with high sensitivity, a compound which is expected to be useful for treating inflammations and sepsis by suppressing the hiNOS expression, or a compound which is expected to be useful for antitumor, antiviral, and vascular restenosis prevention treatments by the hiNOS induction.

摘要翻译： 构建了质粒，其中HiNOS结构基因被作为报告基因的荧光素酶结构基因替代，具有hiNOS基因5'-启动子区和3'非翻译区的保留功能。 将该质粒稳定转染入人细胞系。 上述转化细胞在诱导物存在下选择性地表达报道基因。 通过检查这些转化细胞中的报告基因表达，可以以高灵敏度简单且容易地筛选预期可用于通过抑制hiNOS表达来治疗炎症和败血症的化合物，或者是化合物 预期通过hiNOS诱导对抗肿瘤，抗病毒和血管再狭窄的预防治疗有用。