公开(公告)号：US07442398B2

公开(公告)日：2008-10-28

申请号：US10550758

申请日：2004-03-25

申请人： Young Ok Kim ,  Han Woo Kim ,  Jeong Ho Lee ,  Kyung Kil Kim ,  Jong Yun Lee ,  In Soo Kong

发明人： Young Ok Kim ,  Han Woo Kim ,  Jeong Ho Lee ,  Kyung Kil Kim ,  Jong Yun Lee ,  In Soo Kong

IPC分类号： A23L1/36 ,  C12N9/16 ,  C12P19/30 ,  C12P21/06 ,  C07H21/02 ,  C07H21/04 ,  A23C9/121

CPC分类号： C12N9/16 ,  A23K20/189

摘要： The present invention relates to phytase enzyme, a gene encoding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and the Citrobacter braakii producing the enzyme. The phytase enzyme and the Citrobacter braakii producing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.

摘要翻译： 本发明涉及植酸酶，编码酶的基因和柠檬酸杆菌属。 产生酶。 特别地，本发明涉及由柠檬酸杆菌属（Citrobacter sp。）生产的植酸酶。 （a）分子量为47kDa，（b）最佳pH为3.5-4.5，（c）最适温度为45-55℃，（d）底物植酸盐，磷酸对硝基苯酯，焦磷酸四钠，ATP或 ADP，（e）利用植酸盐作为底物的0.3-0.5mM的米氏常数，和（f）对蛋白酶如胃蛋白酶，胰蛋白酶，木瓜蛋白酶，弹性蛋白酶或胰酶的高抗性。 本发明还涉及编码植酸酶的基因和产生该酶的柠檬酸杆菌（Citrobacter braakii）。 产生本发明酶的植酸酶和柠檬酸杆菌可用于制造作为饲料添加剂的单胃饲料的饲料，并以低价回收植酸盐的特定分解产物。