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公开(公告)号：US20130084602A1
公开(公告)日：2013-04-04
申请号：US13643137
申请日：2011-03-03
申请人： Sang Jun Lee , Young Ok Kim , Bo Hye Nam , Hee Jeong Kong , Kyung Kil Kim
发明人： Sang Jun Lee , Young Ok Kim , Bo Hye Nam , Hee Jeong Kong , Kyung Kil Kim
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/50 , C12N15/70 , C12P21/06
摘要： The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.
摘要翻译： 本发明涉及通过将前导肽与酸性或碱性pI连接来增强异源蛋白质，特别是具有一个或多个跨膜样结构域或分子内二硫键的大体积折叠活性异源蛋白质的可溶性表达和分泌的表达载体和方法 并具有高亲水性; 通过用具有酸性或中性pI和高亲水性的异源蛋白的N-末端取代一个或多个氨基酸; 或降低编码具有碱性pI值和高亲水性的前导肽的多核苷酸的升高GRNA值。 表达载体和方法可以用于产生异源蛋白质并通过防止不溶性包涵体的形成和通过增强异源蛋白质分泌到周质或外部细胞中的分泌效率来转导患者中的治疗性蛋白质。
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公开(公告)号：US09352005B2
公开(公告)日：2016-05-31
申请号：US13885832
申请日：2011-09-08
申请人： Young Ok Kim , Kyung Kil Kim , Bo Hye Nam , Sang Jun Lee , Dong Gyun Kim , Hyon Sob Han , Si Yong Yang , Hee Jeong Kong , Woo Jin Kim , Bong Seok Kim
发明人： Young Ok Kim , Kyung Kil Kim , Bo Hye Nam , Sang Jun Lee , Dong Gyun Kim , Hyon Sob Han , Si Yong Yang , Hee Jeong Kong , Woo Jin Kim , Bong Seok Kim
IPC分类号： A23K1/00 , A23K1/18 , C12R1/07 , A61K35/742
CPC分类号： A61K35/742 , A23K10/18 , A23K50/80 , C12R1/07
摘要： The present invention relates to an isolated Bacillus sp. strain having probiotic activity. More particularly, the present invention relates to an isolated Bacillus sp. 2-4 (KCCM11107P) strain having probiotic activity. Since the strain of the present invention functions as probiotics by having an antibacterial activity against various fish pathogenic bacteria, as well as secreting helpful enzymes such as protease, amylase, cellulose, and lipase to help the digestion and absorption of the feed, the strain of the present invention can be helpfully used as a feed additive for fish and crustaceans.
摘要翻译： 本发明涉及一种分离的芽孢杆菌属 菌株具有益生菌活性。 更具体地说，本发明涉及一种分离的芽孢杆菌属 2-4（KCCM11107P）菌株具有益生菌活性。 由于本发明的菌株通过对各种鱼病原菌具有抗菌活性，以及分泌有用的酶如蛋白酶，淀粉酶，纤维素和脂肪酶来帮助消化和吸收饲料，起到益生菌的作用，因此， 本发明可有效地用作鱼和甲壳动物的饲料添加剂。
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公开(公告)号：US20130302299A1
公开(公告)日：2013-11-14
申请号：US13885832
申请日：2011-09-08
申请人： Young Ok Kim , Kyung Kil Kim , Bo Hye Nam , Sang Jun Lee , Dong Gyun Kim , Hyon Sob Han , Si Yong Yang , Hee Jeong Kong , Woo Jin Kim , Bong Seok Kim
发明人： Young Ok Kim , Kyung Kil Kim , Bo Hye Nam , Sang Jun Lee , Dong Gyun Kim , Hyon Sob Han , Si Yong Yang , Hee Jeong Kong , Woo Jin Kim , Bong Seok Kim
CPC分类号： A61K35/742 , A23K10/18 , A23K50/80 , C12R1/07
摘要： The present invention relates to a novel Bacillus sp. strain having probiotic activity. More particularly, the present disclosure relates to a novel Bacillus sp. 2-4 (KCCM11107P) strain having probiotic activity. Since the strain of the present disclosure functions as probiotics by having an antibacterial activity against various fish pathogenic bacteria, as well as secreting helpful enzymes such as protease, amylase, cellulose, and lipase to help the digestion and absorption of the feed, the strain of the present disclosure can be helpfully used as a feed additive for fish and crustaceans.
摘要翻译： 本发明涉及一种新型芽孢杆菌属 菌株具有益生菌活性。 更具体地，本公开涉及一种新型芽孢杆菌属 2-4（KCCM11107P）菌株具有益生菌活性。 由于本公开的菌株通过对各种鱼病原菌具有抗菌活性，以及分泌有用的酶如蛋白酶，淀粉酶，纤维素和脂肪酶来帮助消化和吸收饲料，起到益生菌作用，因此， 本公开可以有助于用作鱼和甲壳类动物的饲料添加剂。
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公开(公告)号：US07442398B2
公开(公告)日：2008-10-28
申请号：US10550758
申请日：2004-03-25
申请人： Young Ok Kim , Han Woo Kim , Jeong Ho Lee , Kyung Kil Kim , Jong Yun Lee , In Soo Kong
发明人： Young Ok Kim , Han Woo Kim , Jeong Ho Lee , Kyung Kil Kim , Jong Yun Lee , In Soo Kong
CPC分类号： C12N9/16 , A23K20/189
摘要： The present invention relates to phytase enzyme, a gene encoding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and the Citrobacter braakii producing the enzyme. The phytase enzyme and the Citrobacter braakii producing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.
摘要翻译： 本发明涉及植酸酶，编码酶的基因和柠檬酸杆菌属。 产生酶。 特别地，本发明涉及由柠檬酸杆菌属（Citrobacter sp。）生产的植酸酶。 （a）分子量为47kDa，（b）最佳pH为3.5-4.5，（c）最适温度为45-55℃，（d）底物植酸盐，磷酸对硝基苯酯，焦磷酸四钠，ATP或 ADP，（e）利用植酸盐作为底物的0.3-0.5mM的米氏常数，和（f）对蛋白酶如胃蛋白酶，胰蛋白酶，木瓜蛋白酶，弹性蛋白酶或胰酶的高抗性。 本发明还涉及编码植酸酶的基因和产生该酶的柠檬酸杆菌（Citrobacter braakii）。 产生本发明酶的植酸酶和柠檬酸杆菌可用于制造作为饲料添加剂的单胃饲料的饲料，并以低价回收植酸盐的特定分解产物。
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