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    Phytase produced from Citrobacter braakii
    1.
    发明授权
    Phytase produced from Citrobacter braakii 有权
    柠檬酸杆菌生产的植酸酶

    公开(公告)号：US07442398B2

    公开(公告)日：2008-10-28

    申请号：US10550758

    申请日：2004-03-25

    申请人： Young Ok Kim ,  Han Woo Kim ,  Jeong Ho Lee ,  Kyung Kil Kim ,  Jong Yun Lee ,  In Soo Kong

    发明人： Young Ok Kim ,  Han Woo Kim ,  Jeong Ho Lee ,  Kyung Kil Kim ,  Jong Yun Lee ,  In Soo Kong

    IPC分类号： A23L1/36 ,  C12N9/16 ,  C12P19/30 ,  C12P21/06 ,  C07H21/02 ,  C07H21/04 ,  A23C9/121

    CPC分类号： C12N9/16 ,  A23K20/189

    摘要： The present invention relates to phytase enzyme, a gene encoding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and the Citrobacter braakii producing the enzyme. The phytase enzyme and the Citrobacter braakii producing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.

    摘要翻译： 本发明涉及植酸酶，编码酶的基因和柠檬酸杆菌属。 产生酶。 特别地，本发明涉及由柠檬酸杆菌属（Citrobacter sp。）生产的植酸酶。 （a）分子量为47kDa，（b）最佳pH为3.5-4.5，（c）最适温度为45-55℃，（d）底物植酸盐，磷酸对硝基苯酯，焦磷酸四钠，ATP或 ADP，（e）利用植酸盐作为底物的0.3-0.5mM的米氏常数，和（f）对蛋白酶如胃蛋白酶，胰蛋白酶，木瓜蛋白酶，弹性蛋白酶或胰酶的高抗性。 本发明还涉及编码植酸酶的基因和产生该酶的柠檬酸杆菌（Citrobacter braakii）。 产生本发明酶的植酸酶和柠檬酸杆菌可用于制造作为饲料添加剂的单胃饲料的饲料，并以低价回收植酸盐的特定分解产物。

    SOLUBLE EXPRESSION OF BULKY FOLDED ACTIVE PROTEINS
    2.
    发明申请
    SOLUBLE EXPRESSION OF BULKY FOLDED ACTIVE PROTEINS 审中-公开
    大肠杆菌折叠活性蛋白的可溶性表达

    公开(公告)号：US20130084602A1

    公开(公告)日：2013-04-04

    申请号：US13643137

    申请日：2011-03-03

    申请人： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam ,  Hee Jeong Kong ,  Kyung Kil Kim

    发明人： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam ,  Hee Jeong Kong ,  Kyung Kil Kim

    IPC分类号： C12N15/70 ,  C12P21/06

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/70 ,  C12P21/06

    摘要： The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

    摘要翻译： 本发明涉及通过将前导肽与酸性或碱性pI连接来增强异源蛋白质，特别是具有一个或多个跨膜样结构域或分子内二硫键的大体积折叠活性异源蛋白质的可溶性表达和分泌的表达载体和方法 并具有高亲水性; 通过用具有酸性或中性pI和高亲水性的异源蛋白的N-末端取代一个或多个氨基酸; 或降低编码具有碱性pI值和高亲水性的前导肽的多核苷酸的升高GRNA值。 表达载体和方法可以用于产生异源蛋白质并通过防止不溶性包涵体的形成和通过增强异源蛋白质分泌到周质或外部细胞中的分泌效率来转导患者中的治疗性蛋白质。