公开(公告)号：US20090155904A1

公开(公告)日：2009-06-18

申请号：US11721303

申请日：2005-12-08

申请人： Young-Chul Choi ,  Han Oh Park ,  Sorim Choung ,  Young Joo Kim ,  Sang Soo Kim ,  Seong-min Park ,  Sang-Cheol Kim ,  Gyuman Yoon ,  Kyoung Oak Choi ,  Hyo Jin Kang

发明人： Young-Chul Choi ,  Han Oh Park ,  Sorim Choung ,  Young Joo Kim ,  Sang Soo Kim ,  Seong-min Park ,  Sang-Cheol Kim ,  Gyuman Yoon ,  Kyoung Oak Choi ,  Hyo Jin Kang

IPC分类号： C12N5/02

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/11 ,  G16B20/00

摘要： A inhibition method of target mRNA expression includes: (a) obtaining binding energy of a double combination section on a dsRNA sequence of all combination comprising complementary nucleotides to a random target mRNA; (b) dividing the binding energy into four sections on the dsRNA sequence of each combination to obtain a difference of the mean binding energy between each section and convert into a score of a relative combination energy pattern; (c) selecting siRNA whose inhibition efficiency to target mRNA is expected to be high by applying the converted score to the dsRNA sequence with other factors that affect the efficiency of siRNA; and (d) inhibiting target mRNA expression using the selected siRNA. As a result, a researcher or an experimenter can analyze patterns of a relative binding energy on base sequences of unknown siRNA without actual experiments to determine whether the siRNA is effective or ineffective rapidly, thereby design and production efficiency of siRNA can be maximized and target mRNA can be effectively inhibited with efficient siRNA to the target mRNA.

摘要翻译： 靶mRNA表达的抑制方法包括：（a）获得双组合切片对包含与随机靶mRNA的互补核苷酸的所有组合的dsRNA序列的结合能; （b）将结合能分成每个组合的dsRNA序列上的四个部分，以获得每个部分之间的平均结合能的差异，并转换成相对组合能量模式的得分; （c）通过将影响siRNA效率的其它因素应用于dsRNA序列的转化得分，选择siRNA靶向mRNA的抑制效率高的siRNA; 和（d）使用所选择的siRNA抑制靶mRNA表达。 因此，研究人员或实验者可以分析未知siRNA基因序列相对结合能力的模式，无需实际实验，可以快速确定siRNA是否有效或无效，从而可以使siRNA的设计和生产效率最大化，靶mRNA 可以有效地抑制靶mRNA的有效siRNA。