公开(公告)号：US20090155904A1

公开(公告)日：2009-06-18

申请号：US11721303

申请日：2005-12-08

申请人： Young-Chul Choi ,  Han Oh Park ,  Sorim Choung ,  Young Joo Kim ,  Sang Soo Kim ,  Seong-min Park ,  Sang-Cheol Kim ,  Gyuman Yoon ,  Kyoung Oak Choi ,  Hyo Jin Kang

发明人： Young-Chul Choi ,  Han Oh Park ,  Sorim Choung ,  Young Joo Kim ,  Sang Soo Kim ,  Seong-min Park ,  Sang-Cheol Kim ,  Gyuman Yoon ,  Kyoung Oak Choi ,  Hyo Jin Kang

IPC分类号： C12N5/02

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/11 ,  G16B20/00

摘要： A inhibition method of target mRNA expression includes: (a) obtaining binding energy of a double combination section on a dsRNA sequence of all combination comprising complementary nucleotides to a random target mRNA; (b) dividing the binding energy into four sections on the dsRNA sequence of each combination to obtain a difference of the mean binding energy between each section and convert into a score of a relative combination energy pattern; (c) selecting siRNA whose inhibition efficiency to target mRNA is expected to be high by applying the converted score to the dsRNA sequence with other factors that affect the efficiency of siRNA; and (d) inhibiting target mRNA expression using the selected siRNA. As a result, a researcher or an experimenter can analyze patterns of a relative binding energy on base sequences of unknown siRNA without actual experiments to determine whether the siRNA is effective or ineffective rapidly, thereby design and production efficiency of siRNA can be maximized and target mRNA can be effectively inhibited with efficient siRNA to the target mRNA.

摘要翻译： 靶mRNA表达的抑制方法包括：（a）获得双组合切片对包含与随机靶mRNA的互补核苷酸的所有组合的dsRNA序列的结合能; （b）将结合能分成每个组合的dsRNA序列上的四个部分，以获得每个部分之间的平均结合能的差异，并转换成相对组合能量模式的得分; （c）通过将影响siRNA效率的其它因素应用于dsRNA序列的转化得分，选择siRNA靶向mRNA的抑制效率高的siRNA; 和（d）使用所选择的siRNA抑制靶mRNA表达。 因此，研究人员或实验者可以分析未知siRNA基因序列相对结合能力的模式，无需实际实验，可以快速确定siRNA是否有效或无效，从而可以使siRNA的设计和生产效率最大化，靶mRNA 可以有效地抑制靶mRNA的有效siRNA。

公开(公告)号：US20140170769A1

公开(公告)日：2014-06-19

申请号：US14128560

申请日：2012-04-23

申请人： Sang Jeon Chung ,  Hyo Jin Kang ,  Ju Hwan Kim

发明人： Sang Jeon Chung ,  Hyo Jin Kang ,  Ju Hwan Kim

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N33/542 ,  G01N33/6803 ,  G01N2021/6441

摘要： A microscope apparatus for detecting or imaging a target protein using a probe for intrinsic fluorescence resonance energy transfer (iFRET) according to the present invention comprises: a light irradiation unit that irradiates a first light having a wavelength range for exciting an amino acid in the target protein and a second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET; an objective lens that allows the lights irradiated from the light irradiation unit to be incident onto a sample into which the probe for iFRET is introduced; and a recognition unit that detects a first light emitting signal generated from the probe for iFRET by irradiating the first light having a wavelength range for exciting an amino acid in the target protein onto the sample and a second light emitting signal generated from the probe for iFRET by irradiating the second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET onto the sample, wherein the probe for iFRET includes: a binding site specific to a target protein or a molecule which has the binding site; and a fluorescent molecule having an acceptor function with respect to intrinsic fluorescence of the target protein, which are bonded to each other directly or by a linker.

摘要翻译： 根据本发明的用于使用本征荧光共振能量转移（iFRET）的探针检测或成像目标蛋白质的显微镜装置包括：照射具有用于激发靶中的氨基酸的波长范围的第一光的光照射单元 蛋白质和具有用于激发用于iFRET的探针的荧光分子的波长范围的第二光; 允许从光照射单元照射的光入射到其中引入用于iFRET的探针的样品的物镜; 以及识别单元，通过将具有用于激发目标蛋白质中的氨基酸的波长范围的第一光照射到样品上，并且从用于iFRET的探针产生的第二发光信号，检测从探针产生的用于iFRET的第一发光信号 通过将具有用于激发iFRET探针的荧光分子的波长范围的第二光照射在样品上，其中所述iFRET探针包括：对靶蛋白或具有结合位点的分子特异性的结合位点; 以及相对于靶蛋白的固有荧光具有受体功能的荧光分子，它们直接或通过连接体彼此结合。

公开(公告)号：US08236509B2

公开(公告)日：2012-08-07

申请号：US12679982

申请日：2007-10-18

申请人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

发明人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

IPC分类号： G01N33/53

CPC分类号： C07K17/00 ,  C07K7/02 ,  C07K7/08 ,  G01N33/54353 ,  G01N33/54393

摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

摘要翻译： 本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性 蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。 用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。 因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

公开(公告)号：US20140242606A1

公开(公告)日：2014-08-28

申请号：US14128535

申请日：2012-06-25

申请人： Sang Jeon Chung ,  Ju Hwan Kim ,  Elena Ruchkina ,  Hyo Jin Kang

发明人： Sang Jeon Chung ,  Ju Hwan Kim ,  Elena Ruchkina ,  Hyo Jin Kang

IPC分类号： G01N33/58 ,  G01N33/573

CPC分类号： G01N33/582 ,  G01N21/6428 ,  G01N33/542 ,  G01N33/573 ,  G01N2021/6441 ,  G01N2458/00

摘要： The present invention relates to a probe for iFRET and use thereof. Specifically, the present invention relates to a novel probe for iFRET, a method for preparing the probe for iFRET, a method for searching a target protein-specific binding site or a molecule having the binding site using the probe for iFRET, and a method for imaging the target protein using the probe for iFRET. The probe for iFRET according to the present invention utilizes an amino acid in a protein as a fluorescent donor, unlike the conventional FRET method. Therefore, only one fluorescent material is used, and its emission wavelength is distinct from the intrinsic fluorescence of the protein. Thus, high specificity and sensitivity are ensured, and the quantity, activity and mechanism of various proteins can be analyzed in an easy and accurate manner.

摘要翻译： 本发明涉及一种用于iFRET的探针及其用途。 具体地说，本发明涉及用于iFRET的新型探针，用于制备iFRET探针的方法，使用所述iFRET探针来搜索靶蛋白特异性结合位点的方法或具有结合位点的分子，以及用于 使用iFRET的探针成像靶蛋白。 根据本发明的iFRET的探针利用蛋白质中的氨基酸作为荧光供体，与常规的FRET方法不同。 因此，仅使用一种荧光材料，其发射波长与蛋白质的固有荧光不同。 因此，确保了高度的特异性和灵敏度，可以容易且准确地分析各种蛋白质的数量，活性和机理。

公开(公告)号：US20100209945A1

公开(公告)日：2010-08-19

申请号：US12679982

申请日：2007-10-18

申请人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

发明人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

IPC分类号： G01N33/53 ,  C07K7/08 ,  C07K7/06 ,  G01N1/31

CPC分类号： C07K17/00 ,  C07K7/02 ,  C07K7/08 ,  G01N33/54353 ,  G01N33/54393

摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

摘要翻译： 本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说是用于蛋白质固定的肽杂合物，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性 蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。 用于本发明的蛋白质固定的肽杂合物有助于控制抗体在各种固体表面上的取向，并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。 因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

公开(公告)号：US20100196937A1

公开(公告)日：2010-08-05

申请号：US12679830

申请日：2008-05-30

申请人： Sang Jeon Chung ,  Young-mi Lee ,  Yu-Jin Jeong ,  Hyo Jin Kang ,  Bong Hyun Chung

发明人： Sang Jeon Chung ,  Young-mi Lee ,  Yu-Jin Jeong ,  Hyo Jin Kang ,  Bong Hyun Chung

IPC分类号： G01N33/68 ,  G01N33/53

CPC分类号： G01N33/54326 ,  G01N33/581 ,  G01N33/587 ,  G01N2333/976

摘要： The present invention relates to a cascade enzyme-linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.

摘要翻译： 本发明涉及级联酶联免疫吸附测定法，更确切地说，使用固定有靶抗原特异性一级抗体的磁性微粒（MMP）和固定有级联反应引发剂的二氧化硅纳米颗粒（SP）的级联酶联免疫吸附测定法， 抗原特异性二抗。 当将本发明的方法应用于生物样品中的抗原检测时，可以显着提高检测灵敏度。

公开(公告)号：US08642356B2

公开(公告)日：2014-02-04

申请号：US12679830

申请日：2008-05-30

申请人： Sang Jeon Chung ,  Young-mi Lee ,  Yu-Jin Jeong ,  Hyo Jin Kang ,  Bong Hyun Chung

发明人： Sang Jeon Chung ,  Young-mi Lee ,  Yu-Jin Jeong ,  Hyo Jin Kang ,  Bong Hyun Chung

IPC分类号： G01N33/553 ,  G01N33/573

CPC分类号： G01N33/54326 ,  G01N33/581 ,  G01N33/587 ,  G01N2333/976

摘要： The present invention relates to a cascade enzyme-linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.

摘要翻译： 本发明涉及级联酶联免疫吸附测定法，更确切地说，使用固定有靶抗原特异性一级抗体的磁性微粒（MMP）和固定有级联反应引发剂的二氧化硅纳米颗粒（SP）的级联酶联免疫吸附测定法， 抗原特异性二抗。 当将本发明的方法应用于生物样品中的抗原检测时，可以显着提高检测灵敏度。