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公开(公告)号:US5968801A
公开(公告)日:1999-10-19
申请号:US3086
申请日:1998-01-05
申请人: Yuji Saito , Masako Shibayama , Hidehi Takebe , Toshio Matsunobu
发明人: Yuji Saito , Masako Shibayama , Hidehi Takebe , Toshio Matsunobu
IPC分类号: C12N15/09 , C12N1/21 , C12N9/16 , C12N9/18 , C12N15/55 , C12P7/62 , C12R1/15 , C12P21/04 , C12P21/06
摘要: The present invention relates to polyhydroxyalkanoate depolymerase having a N-terminal fragment of the amino acid sequence of SEQ ID NO: 1 and having a molecular weight of about 33,000 as determined by SDS polyacrylamide gel electrophoresis; a process for producing polyhydroxyalkanoate depolymerase, comprising culturing in a medium a microorganism which belongs to the genus Corynebacterium and has the ability to produce the polyhydroxyalkanoate depolymerase and recovering the polyhydroxyalkanoate depolymerase from the resulting culture; and a process for producing polyhydroxyalkanoate depolymerase, comprising culturing in a medium a transformant transformed with a recombinant vector containing a gene of the polyhydroxyalkanoate depolymerase and recovering the polyhydroxyalkanoate depolymerase from the resulting culture. According to the present invention, there can be provided novel PHA depolymerase with the activity of decomposing .omega.-hydroxyalkanoates, particularly 4HB homopolyester or copolymerized polyesters containing said homopolyester, and a process of efficiently producing the enzyme.
摘要翻译: 本发明涉及通过SDS聚丙烯酰胺凝胶电泳测定的具有SEQ ID NO:1的氨基酸序列的N-末端片段并具有约33,000的分子量的聚羟基链烷酸酯解聚酶; 一种生产聚羟基链烷酸酯解聚酶的方法,包括在培养基中培养属于棒杆菌属的微生物并具有产生聚羟基链烷酸酯解聚酶的能力,并从所得培养物中回收聚羟基链烷酸酯解聚酶; 以及生产聚羟基链烷酸酯解聚酶的方法,包括在培养基中培养由含有聚羟基链烷酸酯解聚酶基因的重组载体转化的转化体,并从得到的培养物中回收聚羟基链烷酸酯解聚酶。 根据本发明,可以提供具有分解ω-羟基链烷酸酯的活性的新型PHA解聚酶,特别是含有所述均聚酯的4HB均聚酯或共聚聚酯,以及有效生产该酶的方法。