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公开(公告)号：US07008651B2
公开(公告)日：2006-03-07
申请号：US09848449
申请日：2001-05-03
CPC分类号： C07K14/245 , C12P21/02
摘要： There is now a market for kits containing the necessary components for performing protein synthesis reactions using template DNA of the experimenter. One category of existing system for performing protein synthesis reactions is based on an S-30 extract from the bacteria E. coli. It is disclosed here that a simple fractionation process can dramatically improve the performance of an S-30 prokaryotic protein synthesis reaction mixture. In one embodiment, the fractionation is a simple freezing and thawing of an S-30 extract combined with a supplemental mix, followed by centrifugation. The resulting fractionated S-30 reaction mixture yields more full-length target protein and less non-full length or non-target protein than possible using prior art S-30 systems.
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公开(公告)号：US5629179A
公开(公告)日：1997-05-13
申请号：US406089
申请日：1995-03-17
CPC分类号： C12Q1/6855 , C12N15/1096
摘要： A method for forming directionally clonable randomly primed cDNA molecules includes the step of priming first strand synthesis using a set of first strand cDNA primers having the sequence 5'-XXNNNNNN-3' where XX is a constant dinucleotide pair across the set and NNNNNN is a random hexanucleotide, the set including primers representing all random hexanucleotides.
摘要翻译： 用于形成定向克隆的随机引物的cDNA分子的方法包括使用一组具有序列5'-XXNNNNNN-3'的第一链cDNA引物引发第一链合成的步骤，其中XX是整个组中的常数二核苷酸对，NNNNNN是 随机六核苷酸，该组包括表示所有随机六核苷酸的引物。
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公开(公告)号：US5856144A
公开(公告)日：1999-01-05
申请号：US878379
申请日：1997-06-18
CPC分类号： C12N15/66
摘要： A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.
摘要翻译： 用于直接克隆PCR方法产物的载体在线性化DNA片段的一端或两端掺入单核苷酸突出端。 根据需要，单核苷酸突出端是尿嘧啶或肌苷残基，以促进所需PCR产物的克隆。
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