公开(公告)号：US12189110B2

公开(公告)日：2025-01-07

申请号：US17691125

申请日：2022-03-10

Applicant: ZHEJIANG LAB ,  ZHEJIANG UNIVERSITY

Inventor： Cuihong Li ,  Zhenhai Fu ,  Jing Jiang ,  Zhiming Chen ,  Yuanyuan Ma ,  Huizhu Hu

IPC: G02B21/32 ,  G21K1/00 ,  G01N15/00

Abstract: Disclosed is an optical trap calibration apparatus and method based on variation of electric field by optical imaging of a nanoparticle. By means of a direct optical imaging method, a linear nanoparticle equilibrium position displacement under the action of a constant electric field is measured to realize calibration, thereby avoiding the introduction of error signals, and improving the reliability of differential calibration. The specific calibration method and apparatus of the present invention are not only suitable for calibration of electric field quantity, but also suitable for the calibration of other magnetic forces and the like. By means of the accurate calibration of mechanical quantity in the present invention, the development and application of the vacuum optical trap sensing technology can be promoted.

公开(公告)号：US11789057B2

公开(公告)日：2023-10-17

申请号：US18089210

申请日：2022-12-27

Applicant: Zhejiang Lab ,  Zhejiang University

Inventor： Huizhu Hu ,  Zhenhai Fu ,  Xiaowen Gao ,  Tao Liang ,  Peitong He ,  Jing Jiang

IPC: G01R29/12

CPC classification number: G01R29/12

Abstract: A method for electric field measurement based on a levitated particle includes steps of (1) capturing a particle and levitating the captured particle; (2) adjusting a quantity of electric charge carried by the levitated particle; (3) measuring a charge number N of the levitated particle; (4) disposing the levitated particle in an electric field to be measured, measuring a displacement power spectral density Svxel of the levitated particle under the electric field and obtaining an electric field force Fel; and (5) according to a formula of E=Fel/Nqe, obtaining an electric field intensity E. An apparatus for electric field measurement based on a levitated particle includes a high-voltage DC (direct current) power supply, two bare wire electrodes, a vacuum chamber, a trapping laser, an objective lens, a pair of parallel electrodes, a collective lens, a quadrant photodetector, a lock-in amplifier, a signal generator and a power amplifier.

公开(公告)号：US20230135076A1

公开(公告)日：2023-05-04

申请号：US18089210

申请日：2022-12-27

Applicant: Zhejiang Lab ,  Zhejiang University

Inventor： Huizhu Hu ,  Zhenhai Fu ,  Xiaowen Gao ,  Tao Liang ,  Peitong He ,  Jing Jiang

IPC: G01R29/12

Abstract: A method for electric field measurement based on a levitated particle includes steps of (1) capturing a particle and levitating the captured particle; (2) adjusting a quantity of electric charge carried by the levitated particle; (3) measuring a charge number N of the levitated particle; (4) disposing the levitated particle in an electric field to be measured, measuring a displacement power spectral density Svxel of the levitated particle under the electric field and obtaining an electric field force Fel; and (5) according to a formula of E=Fel/Nqe, obtaining an electric field intensity E. An apparatus for electric field measurement based on a levitated particle includes a high-voltage DC (direct current) power supply, two bare wire electrodes, a vacuum chamber, a trapping laser, an objective lens, a pair of parallel electrodes, a collective lens, a quadrant photodetector, a lock-in amplifier, a signal generator and a power amplifier.

公开(公告)号：US20190209712A1

公开(公告)日：2019-07-11

申请号：US16355723

申请日：2019-03-16

Applicant: Zhejiang University

Inventor： Jun Li ,  Suwan Sun ,  Jiang Li ,  Jing Jiang ,  Ningshao Xia ,  Tong Cheng ,  Lunzhi Yuan

IPC: A61K49/00 ,  A61K35/28 ,  A61K35/76 ,  A01K67/027

CPC classification number: A61K49/0008 ,  A01K67/0278 ,  A01K2227/105 ,  A01K2267/0337 ,  A61K35/28 ,  A61K35/76

Abstract: A humanized murine model of chronic hepatitis B constructed using human stem cells, obtained by obtaining the human stem cells; transplanting the human stem cells into a murine with liver damage to achieve presence of 1×104-8 of the human stem cells in the murine in vivo; etc. The humanized murine model may be used for the study of drug resistance to the antiviral drugs, the study of the pathogenesis of chronic hepatitis B, etc. In addition to the study of the mechanism of hepatitis B viral infection, the humanized murine model may also be used for research in treatment.

公开(公告)号：US20190208754A1

公开(公告)日：2019-07-11

申请号：US16355707

申请日：2019-03-16

Applicant: Zhejiang University

Inventor： Jun Li ,  Suwan Sun ,  Jiang Li ,  Jing Jiang

IPC: A01K67/027 ,  A61K9/00 ,  C12N5/0735

CPC classification number: A01K67/0278 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/20 ,  A01K2227/105 ,  A01K2227/106 ,  A01K2267/0337 ,  A61K9/0019 ,  A61K9/0053 ,  C12N5/0606

Abstract: A method of constructing humanized murine model using stem cells, includes obtaining human stem cells; transplanting human stem cells into murine with liver damage; and obtaining hepatotropic virus infected humanized murine. It was found that by inducing severe liver damage and transplanting human stem cells, human-derived hepatocytes in murine liver have a high chimeric rate of 50-95%, and human-derived immune cells may exist in murine organs such as spleen, blood, liver, and bone marrow, thereby forming murine model of humanized liver and immune cell. The humanized murines are then infected with various types of hepatotropic viruses to form humanized hepatotropic viral infected murine model. In addition to construction of model for studying hepatotropic viral infection using the technique for constructing the humanized murine model, the concept of this technical solution may also be used for constructing models of other humanized organs.