公开(公告)号：US20180195119A1

公开(公告)日：2018-07-12

申请号：US15867031

申请日：2018-01-10

申请人： Zhitong LIU ,  Guoying LIU ,  Jeffrey Juehui LIU ,  Tao CHEN

发明人： Zhitong LIU ,  Guoying LIU ,  Jeffrey Juehui LIU ,  Tao CHEN

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6848

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q2521/325 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/159 ,  C12Q2563/179

摘要： Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA. The methods, compositions, systems and kits described herein may include, or include the use of, one or more single-stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.

公开(公告)号：US20170022551A1

公开(公告)日：2017-01-26

申请号：US15290981

申请日：2016-10-11

申请人： Zhitong LIU ,  Jeffrey Juehui LIU ,  Guoying LIU ,  Tao CHEN

发明人： Zhitong LIU ,  Jeffrey Juehui LIU ,  Guoying LIU ,  Tao CHEN

IPC分类号： C12Q1/68

摘要： Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.

摘要翻译： 当扩增多个不同核苷酸区域时，通过减少非特异性扩增产物（例如引物二聚体）来扩增或改善靶特异性扩增产物的扩增的方法，组合物，系统和试剂盒。 本文描述的方法，组合物，系统和试剂盒可以包括或包括使用识别并结合和/或切割异常DNA结构的一种或多种解析酶。