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1.发明申请REGULATORY ELEMENT FOR HETEROLOGOUS PROTEIN PRODUCTION IN THE FRUITING BODY OF FILAMENTOUS FUNGI 有权FILAMENTOUS FUNGI水果体内异位蛋白质生产的调节元素公开(公告)号：US20130219554A1
公开(公告)日：2013-08-22
申请号：US13819356
申请日：2011-08-30
IPC分类号： C12N15/80 , C12N15/113
CPC分类号： C12N15/80 , C07K14/37 , C07K14/8117 , C12N15/113
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.
摘要翻译： 本发明提供调节真菌中核苷酸序列表达的组合物和方法。 组合物是从双孢蘑菇凝集素基因分离的组织优选启动子的新核苷酸序列。 序列优先驱动表达水果体组织。 提供了使用本文公开的调节序列在真菌中表达核苷酸序列的方法。 该方法包括转化真菌细胞以包含可操作地连接到本发明的一个或多个调节序列的核苷酸序列，并从转化的细胞再生稳定转化的真菌。
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2.发明授权公开(公告)号：US09145561B2
公开(公告)日：2015-09-29
申请号：US13819356
申请日：2011-08-30
IPC分类号： C07H21/04 , C12N15/00 , C12N1/15 , C12N15/81 , C12N15/80 , C12N15/113 , C07K14/81 , C07K14/37
CPC分类号： C12N15/80 , C07K14/37 , C07K14/8117 , C12N15/113
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.
摘要翻译： 本发明提供调节真菌中核苷酸序列表达的组合物和方法。 组合物是从双孢蘑菇凝集素基因分离的组织优选启动子的新核苷酸序列。 序列优先驱动表达水果体组织。 提供了使用本文公开的调节序列在真菌中表达核苷酸序列的方法。 该方法包括转化真菌细胞以包含可操作地连接到本发明的一个或多个调节序列的核苷酸序列，并从转化的细胞再生稳定转化的真菌。
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3.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US07705215B1
公开(公告)日：2010-04-27
申请号：US08113561
申请日：1993-08-25
申请人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
发明人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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4.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US06399861B1
公开(公告)日：2002-06-04
申请号：US08447985
申请日：1995-05-23
申请人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
发明人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
IPC分类号： A01H500
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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5.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US06803499B1
公开(公告)日：2004-10-12
申请号：US08852340
申请日：1997-05-07
申请人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
发明人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
IPC分类号： C12N504
CPC分类号： A01H4/001 , A01H4/00 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N15/8207 , C12N15/8241 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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