公开(公告)号：US07897745B2

公开(公告)日：2011-03-01

申请号：US11958795

申请日：2007-12-18

申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8216

摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包含可操作地连接到具有终止密码子的编码序列和真菌终止序列的植物启动子。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。

公开(公告)号：US07563863B2

公开(公告)日：2009-07-21

申请号：US12015353

申请日：2008-01-16

申请人： Michael Lassner ,  Jack Q. Wilkinson

发明人： Michael Lassner ,  Jack Q. Wilkinson

IPC分类号： C07K5/00 ,  C07K14/00 ,  C07H21/00 ,  C12N15/63 ,  C12N15/82 ,  A01H5/00

CPC分类号： C07K14/415 ,  C07K14/00 ,  C12N15/8221

摘要： The present invention provides novel plastid transit peptides that direct localization of attached moieties (e.g., polypeptides) into plant plastids. The present invention also relates to methods and compositions for localizing polypeptides to plant plastids including, but not limited to, transgenic plant production.

公开(公告)号：US20080234130A1

公开(公告)日：2008-09-25

申请号：US11507751

申请日：2006-08-22

申请人： Billy Fred McCutchen ,  Linda A. Castle ,  Timothy K. Chicoine ,  Hyeon-Je Cho ,  Jon S. Claus ,  Jerry M. Green ,  Anthony D. Guida ,  Christine B. Hazel ,  Matthew J. Heckert ,  Jeffrey M. Hegstad ,  James M. Hutchison ,  Donglong Liu ,  Albert L. Lu ,  Wayne J. Mehre ,  York Moy ,  Paul D. Olson ,  Kenneth A. Peeples ,  David W. Saunders ,  Mark D. Vogt ,  Jack Q. Wilkinson ,  James F. H. Wong

发明人： Billy Fred McCutchen ,  Linda A. Castle ,  Timothy K. Chicoine ,  Hyeon-Je Cho ,  Jon S. Claus ,  Jerry M. Green ,  Anthony D. Guida ,  Christine B. Hazel ,  Matthew J. Heckert ,  Jeffrey M. Hegstad ,  James M. Hutchison ,  Donglong Liu ,  Albert L. Lu ,  Wayne J. Mehre ,  York Moy ,  Paul D. Olson ,  Kenneth A. Peeples ,  David W. Saunders ,  Mark D. Vogt ,  Jack Q. Wilkinson ,  James F. H. Wong

IPC分类号： A01N43/48 ,  A01N43/64 ,  A01N57/18 ,  A01N43/647 ,  A01P13/00 ,  C12N15/11 ,  A01N59/26

CPC分类号： C12N15/8274 ,  C12N9/1092 ,  C12N9/88 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8271 ,  C12N15/8275 ,  C12N15/8278 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

摘要翻译： 提供了与多于一种除草剂耐受的改良植物相关的方法和组合物。 特别地，本发明提供了耐草甘膦并且耐受至少一种ALS抑制剂的植物及其使用方法。 草甘膦/ ALS抑制剂耐受性植物包含编码赋予对草甘膦的耐受性的多肽的多核苷酸和编码ALS抑制剂耐受性多肽的多核苷酸。 在具体实施方案中，本发明的植物表达GAT多肽和HRA多肽。 提供了控制杂草，提高植物产量和提高转化效率的方法。

公开(公告)号：US20080178352A1

公开(公告)日：2008-07-24

申请号：US12015342

申请日：2008-01-16

申请人： Michael Lassner ,  Jack Q. Wilkinson

发明人： Michael Lassner ,  Jack Q. Wilkinson

IPC分类号： C12N15/62 ,  C12N15/11 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00

CPC分类号： C07K14/415 ,  C07K14/00 ,  C12N15/8221

摘要： The present invention provides novel plastid transit peptides that direct localization of attached moieties (e.g., polypeptides) into plant plastids. The present invention also relates to methods and compositions for localizing polypeptides to plant plastids including, but not limited to, transgenic plant production.

公开(公告)号：US20080168580A1

公开(公告)日：2008-07-10

申请号：US12015353

申请日：2008-01-16

申请人： Michael Lassner ,  Jack Q. Wilkinson

发明人： Michael Lassner ,  Jack Q. Wilkinson

IPC分类号： C12N15/11 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00

CPC分类号： C07K14/415 ,  C07K14/00 ,  C12N15/8221

摘要： The present invention provides novel plastid transit peptides that direct localization of attached moieties (e.g., polypeptides) into plant plastids. The present invention also relates to methods and compositions for localizing polypeptides to plant plastids including, but not limited to, transgenic plant production.

公开(公告)号：US20080127377A1

公开(公告)日：2008-05-29

申请号：US11959943

申请日：2007-12-19

申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

IPC分类号： A01H5/00 ,  C12N15/00 ,  C07H21/04 ,  C12N5/02

CPC分类号： C12N15/8216

摘要： The present invention relates to heterologous genes comprising non-plant 3′ termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

公开(公告)号：US06919495B2

公开(公告)日：2005-07-19

申请号：US10427169

申请日：2003-05-01

申请人： Karen L. Fincher ,  Stanislaw Flasinski ,  Jack Q. Wilkinson

发明人： Karen L. Fincher ,  Stanislaw Flasinski ,  Jack Q. Wilkinson

IPC分类号： A01H5/00 ,  C12N15/09 ,  C12N15/82 ,  A01H1/00 ,  C12N15/00 ,  C12N5/04 ,  C07H21/04

CPC分类号： C12N15/8275 ,  A01N57/20 ,  C12N7/00 ,  C12N9/1092 ,  C12N15/8216 ,  C12N15/8221 ,  C12N15/8222 ,  C12N15/8223 ,  C12N15/823 ,  C12N15/8261 ,  C12N15/8286 ,  C12N2710/00043 ,  Y02A40/146 ,  Y02A40/162

摘要： The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.

摘要翻译： 本发明涉及新型植物表达构建体。 更具体地，本发明提供了包含用于调节植物中可操作连接的基因的表达的5'调节序列的DNA构建体。

公开(公告)号：US06462258B1

公开(公告)日：2002-10-08

申请号：US09737698

申请日：2000-12-15

申请人： Karen L. Fincher ,  Jack Q. Wilkinson

发明人： Karen L. Fincher ,  Jack Q. Wilkinson

IPC分类号： A01H100

CPC分类号： C12N15/8275 ,  A01N57/20 ,  C12N7/00 ,  C12N9/1092 ,  C12N15/8216 ,  C12N15/8221 ,  C12N15/8222 ,  C12N15/8223 ,  C12N15/823 ,  C12N15/8261 ,  C12N15/8286 ,  C12N2710/00043 ,  Y02A40/146 ,  Y02A40/162

摘要： The present invention relates to a novel plant expression construct. More specifically the present invention provides a DNA construct comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.

摘要翻译： 本发明涉及一种新的植物表达构建体。 更具体地，本发明提供了包含用于调节植物中可操作连接的基因的表达的5'调节序列的DNA构建体。

公开(公告)号：US07851613B2

公开(公告)日：2010-12-14

申请号：US11960919

申请日：2007-12-20

申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8216

摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop condon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包含可操作地连接到具有停止螺旋的编码序列的植物启动子和真菌终止序列。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。

公开(公告)号：US07847086B2

公开(公告)日：2010-12-07

申请号：US11961334

申请日：2007-12-20

申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8216

摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包含可操作地连接到具有终止密码子的编码序列和真菌终止序列的植物启动子。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。