利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.022 秒)

    Methods for Agrobacterium-mediated transformation
    1.
    发明授权
    Methods for Agrobacterium-mediated transformation 失效
    农杆菌介导转化方法

    公开(公告)号：US06822144B1

    公开(公告)日：2004-11-23

    申请号：US08963096

    申请日：1997-11-03

    申请人： Zuo-Yu Zhao ,  Weining Gu ,  Tishu Cai ,  Dorothy A. Pierce

    发明人： Zuo-Yu Zhao ,  Weining Gu ,  Tishu Cai ,  Dorothy A. Pierce

    IPC分类号： C12N1584

    CPC分类号： C12N15/8205

    摘要： The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

    摘要翻译： 本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。 使用农杆菌转化玉米的优选方法包括以下步骤：使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎，能够抑制农杆菌生长的抗生素，以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。

    Methods for agrobacterium-mediated transformation
    2.
    发明授权
    Methods for agrobacterium-mediated transformation 失效
    农杆菌介导转化方法

    公开(公告)号：US5981840A

    公开(公告)日：1999-11-09

    申请号：US788018

    申请日：1997-01-24

    申请人： Zuo-Yu Zhao ,  Weining Gu ,  Tishu Cai ,  Dorothy A. Pierce

    发明人： Zuo-Yu Zhao ,  Weining Gu ,  Tishu Cai ,  Dorothy A. Pierce

    IPC分类号： C12N15/82 ,  C12N15/84 ,  C12N15/00 ,  A01H5/00 ,  C12N15/05

    CPC分类号： C12N15/8205

    摘要： The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

    摘要翻译： 本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。 使用农杆菌转化玉米的优选方法包括以下步骤：使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎，能够抑制农杆菌生长的抗生素，以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。

    Agrobacterium mediated transformation of sorghum
    3.
    发明授权
    Agrobacterium mediated transformation of sorghum 失效
    农杆菌介导的高粱转化

    公开(公告)号：US06369298B1

    公开(公告)日：2002-04-09

    申请号：US09056418

    申请日：1998-04-07

    申请人： Tishu Cai ,  Dorothy A. Pierce ,  Laura A. Tagliani ,  Zuo-Yu Zhao

    发明人： Tishu Cai ,  Dorothy A. Pierce ,  Laura A. Tagliani ,  Zuo-Yu Zhao

    IPC分类号： A01H100

    CPC分类号： C12N15/8205

    摘要： Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.

    摘要翻译： 提供了高效转化高粱的方法和组成。 该方法涉及用农杆菌感染，特别是包含超二元载体的那些。 以这种方式，可以将任何感兴趣的基因引入高粱植物中。 转化的基因将侧接至少一个T-DNA边界并以低拷贝数存在于转化的高粱中。 还提供了转化的高粱，细胞，组织，植物和种子。 本发明包括再生的，可育的高粱植物，由其产生的转基因种子，T1和后代。

    Plant QM proteins
    6.
    发明授权
    Plant QM proteins 失效
    植物QM蛋白

    公开(公告)号：US5760190A

    公开(公告)日：1998-06-02

    申请号：US472263

    申请日：1995-06-07

    申请人： John Neill ,  Dorothy A. Pierce ,  Andrew M. Cigan

    发明人： John Neill ,  Dorothy A. Pierce ,  Andrew M. Cigan

    IPC分类号： A01H1/00 ,  C07K14/415 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12P21/02 ,  C12R1/91 ,  A61K35/78

    CPC分类号： C12N15/8222 ,  C07K14/415 ,  C12N15/8231 ,  C12N15/8289

    摘要： A family of genes has been found in plants, said genes encoding a family of developmental proteins that have homologous structures to the mammalian QM genes. Recombinant molecules which include the QM genes in plants are useful to transform cells and regenerate plants that, as a result, have altered developmental pathways. Methods of producing male sterile plants use recombinant molecules containing either the QM sense genes or antesense genes with appropriate promoters.

    摘要翻译： 已经在植物中发现了一系列基因，所述基因编码与哺乳动物QM基因具有同源结构的发育蛋白家族。 在植物中包括QM基因的重组分子可用于转化细胞并再生植物，结果改变了发育途径。 产生雄性不育植物的方法使用含有QM有义基因或反义基因的重组分子与合适的启动子。

    Use of the green fluorescent protein as a screenable marker for plant transformation
    7.
    发明授权
    Use of the green fluorescent protein as a screenable marker for plant transformation 失效
    使用绿色荧光蛋白作为植物转化的可筛选标记

    公开(公告)号：US06486382B1

    公开(公告)日：2002-11-26

    申请号：US09214909

    申请日：1999-12-20

    申请人： William Gordan-Kamm ,  Dorothy A. Pierce ,  Benjamin Bowen ,  Dennis Bidney ,  Margit Ross ,  Christopher Scelonge ,  Michael D. Miller ,  Gary Sandahl ,  Lijuan Wang

    发明人： William Gordan-Kamm ,  Dorothy A. Pierce ,  Benjamin Bowen ,  Dennis Bidney ,  Margit Ross ,  Christopher Scelonge ,  Michael D. Miller ,  Gary Sandahl ,  Lijuan Wang

    IPC分类号： C12N1582

    CPC分类号： C12N15/8216 ,  C07K14/43595 ,  C12N15/8209 ,  C12N15/8212 ,  C12N15/8213

    摘要： A method for the production of transgenic plants is provided in which a vector carrying a gene encoding the green fluorescent protein is introduced into cells, the cells are screened for the protein and transformed cells are selected and regenerated. The cellular toxicity of the green fluorescent protein is circumvented by regulating expression of the gene encoding the protein or directing the protein to a subcellular compartment where it is not toxic to the cell. DNA constructs are provided for cell transformation in which the expression of a gene encoding the green fluorescent protein is placed under the control of an inducible promoter. In addition, DNA constructs are provided in which a nucleotide sequence encoding the green fluorescent protein is operably linked to a signal sequence which directs the expressed protein to a subcellular compartment where the protein is not toxic to the cell. Oxidative stress to plant cells transformed with GFP also can be ameliorated by transforming cells with an expression vector comprising genes encoding GFP and an oxygen scavenger enzyme such as superoxide dismutase. The toxicity of GFP in transformed plants can be eliminated by excising the screenable marker gene following detection of transformed cells or sectors. The FLP/FRT system is used in conjunction with GFP as a visible marker for transformation and FRT excision. A nucleotide sequence optimized for expression of the green fluorecent protein in plants is also provided.

    摘要翻译： 提供了一种用于生产转基因植物的方法，其中将携带编码绿色荧光蛋白的基因的载体导入细胞，筛选细胞以进行蛋白质，并选择和再生转化的细胞。 绿色荧光蛋白的细胞毒性通过调节编码蛋白质的基因的表达或引导蛋白质转移到对细胞无毒性的亚细胞区域来避免。 提供DNA构建体用于细胞转化，其中将编码绿色荧光蛋白的基因的表达置于诱导型启动子的控制之下。 此外，提供了DNA构建体，其中编码绿色荧光蛋白的核苷酸序列可操作地连接到将表达的蛋白质导向亚细胞区域的信号序列，其中蛋白质对细胞无毒性。 用GFP转化的植物细胞的氧化应激也可以通过用包含编码GFP和氧清除酶如超氧化物歧化酶的基因的表达载体转化细胞来改善。 通过在检测到转化的细胞或部分后切除可筛选标记基因，可以消除GFP在转化植物中的毒性。 FLP / FRT系统与GFP结合使用，作为转化和FRT切除的可见标记。 还提供了优化用于植物中绿色荧光蛋白表达的核苷酸序列。