公开(公告)号：US11993637B2

公开(公告)日：2024-05-28

申请号：US17165351

申请日：2021-02-02

申请人： AMBRX, INC.

发明人： Thomas P. Cujec ,  Roberto Mariani ,  Anna-Maria A. Hays Putnam ,  William M. Keefe ,  Nick Knudsen ,  Lillian Skidmore ,  Jason Pinkstaff ,  Vadim Kraynov

IPC分类号： C07K14/50 ,  A61K38/00 ,  A61K38/18 ,  A61K45/06 ,  A61K47/60 ,  C12N15/70

CPC分类号： C07K14/50 ,  A61K38/1825 ,  A61K45/06 ,  A61K47/60 ,  C12N15/70 ,  A61K38/00 ,  Y02A50/30

摘要： Modified FGF-21 polypeptides and uses thereof are provided.

公开(公告)号：US11851662B2

公开(公告)日：2023-12-26

申请号：US16958152

申请日：2018-06-02

申请人： Ambrx, Inc.

发明人： Sigeng Chen ,  Yingchun Lu ,  Feng Tian

IPC分类号： C12N15/67 ,  C12N5/10 ,  C12N15/11

CPC分类号： C12N15/67 ,  C12N5/10 ,  C12N15/11 ,  C12N2310/20 ,  C12N2501/48 ,  C12N2510/02

摘要： Disclosed herein are methods and compositions for generation of cell lines to promote unnatural amino acid-containing protein production using genome engineering technology.

公开(公告)号：US20230302150A1

公开(公告)日：2023-09-28

申请号：US18022099

申请日：2021-08-20

申请人： Ambrx, Inc.

发明人： Sung Ju Moon ,  Brian Leon ,  Mingchao Kang ,  Nickolas Knudsen ,  Sukumar Sakamuri ,  Feng Tian

IPC分类号： A61K47/68 ,  A61K47/60 ,  A61K45/06 ,  A61P35/00

CPC分类号： A61K47/6851 ,  A61K47/60 ,  A61K47/6849 ,  A61K47/6869 ,  A61K45/06 ,  A61P35/00

摘要： Disclosed herein are TLR-agonists compounds, antibody-TLR agonist conjugates, pharmaceutical composition, and methods of use of such compounds or conjugates as therapeutics for treating a disease or condition such as cancer.

公开(公告)号：US20230295320A1

公开(公告)日：2023-09-21

申请号：US17898384

申请日：2022-08-29

申请人： Ambrx, Inc.

发明人： Richard S Barnett ,  Nickolas Knudsen ,  Ying Sun ,  Sandra Biroc ,  Timothy Buss ,  Tsotne Javahishvili ,  Damien Bresson ,  Shailaja Srinagesh ,  Amha Hewet ,  Jason Pinkstaff

IPC分类号： C07K16/28 ,  A61K47/68 ,  A61K47/42 ,  C07K5/062

CPC分类号： C07K16/2875 ,  A61K47/6889 ,  A61K47/6817 ,  A61K47/6845 ,  A61K47/42 ,  C07K5/06052 ,  C07K2317/76

摘要： This invention relates to anti-CD70 antibodies and antibody drug conjugates comprising at least one non-naturally-encoded amino acid. Disclosed herein are aCD70 antibodies with one or more non-naturally encoded amino acids and further disclosed are antibody drug conjugates wherein the aCD70 antibodies of the invention are conjugated to one or more toxins. Further disclosed are methods for using such non-natural amino acid antibody drug conjugates, including therapeutic, diagnostic, and other biotechnology uses.

公开(公告)号：US20220056093A1

公开(公告)日：2022-02-24

申请号：US17275662

申请日：2019-09-11

申请人： Ambrx, Inc.

发明人： Sigeng CHEN ,  Yingchun Lu ,  MD Harunur Rashid ,  Nickolas Knudsen ,  Feng Tian

IPC分类号： C07K14/55 ,  A61K47/60

摘要： The present invention provides methods for targeting interleukin-2 receptor-expressing cells, and, in particular, inhibiting the growth of such cells by using an interleukin-2 (IL-2) variant conjugated to a biologically active molecule that will affect cells expressing the interleukin-2 receptor.

公开(公告)号：US10800856B2

公开(公告)日：2020-10-13

申请号：US14406190

申请日：2013-06-07

申请人： Ambrx, Inc.

发明人： Richard S. Barnett ,  Feng Tian ,  Anna-Maria A. Hays Putnam ,  Marco Gymnopoulos ,  Nick Knudsen ,  Andrew Beck ,  Ying Sun

IPC分类号： A61K47/68 ,  A61K49/00 ,  C07K16/40 ,  C07K16/30 ,  C07K5/02

摘要： This invention relates to prostate-specific membrane antigen (PSMA) antibodies and antibody drug conjugates comprising at least one non-naturally-encoded amino acid. Disclosed herein are αPSMA antibodies with one or more non-naturally encoded amino acids and further disclosed are antibody drug conjugates wherein the αPSMA antibodies of the invention are conjugated to one or more toxins. Also disclosed herein are non-natural amino acid dolastatin analogs that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such dolastatin analogs. Typically, the modified dolastatin analogs include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid antibody drug conjugates, dolastatin analogs, and modified non-natural amino acid dolastatin analogs, including therapeutic, diagnostic, and other biotechnology uses.

公开(公告)号：US10751391B2

公开(公告)日：2020-08-25

申请号：US16285986

申请日：2019-02-26

申请人： AMBRX, INC.

发明人： Vadim Kraynov ,  Nick Knudsen ,  Amha Hewet ,  Kristine De Dios ,  Jason Pinkstaff ,  Lorraine Sullivan

IPC分类号： A61K38/18 ,  A61K38/22 ,  C07K14/64

摘要： Modified relaxin polypeptides and their uses thereof are provided. Exemplary embodiments provide relaxin polypeptides which include one or more amino acid substitutions with natural or non-naturally encoded amino acids, and/or linkage to a water-soluble polymer, such as polyethylene glycol. Additionally, use of said relaxin polypeptides for treatment of disease, such as heart failure, is also provided.

公开(公告)号：US09868972B2

公开(公告)日：2018-01-16

申请号：US14820318

申请日：2015-08-06

申请人： Ambrx, Inc.

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12P21/00 ,  C12P21/02 ,  C12N9/00 ,  C12N15/11

CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/11

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.

公开(公告)号：US09517273B2

公开(公告)日：2016-12-13

申请号：US14680543

申请日：2015-04-07

申请人： Ambrx, Inc.

发明人： Thomas P. Cujec ,  Roberto Mariani ,  Anna-Maria A. Hays Putnam ,  Wiliam M. Keefe ,  Nick Knudsen ,  Lillian Skidmore ,  Jason Pinkstaff ,  Vadim Kraynov

IPC分类号： A61K38/18 ,  C12P21/00 ,  C07K14/50 ,  A61K47/48 ,  A61K45/06 ,  A61K38/00

CPC分类号： C07K14/50 ,  A61K38/00 ,  A61K38/1825 ,  A61K45/06 ,  A61K47/60 ,  C12N15/70 ,  Y02A50/473

摘要： Modified FGF-21 polypeptides and uses thereof are provided.

摘要翻译： 提供了修饰的FGF-21多肽及其用途。

公开(公告)号：US09133495B2

公开(公告)日：2015-09-15

申请号：US12440015

申请日：2007-09-07

申请人： Feng Tian ,  Thea Norman ,  Stephanie Chu

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12N15/85 ,  C12P21/02 ,  C12N9/00 ,  C12N15/11

CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/11

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.

摘要翻译： 本发明提供用于生产翻译成分的组合物和方法，其扩展脊椎动物细胞中遗传编码氨基酸的数目。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸的正交对。 还提供了在脊椎动物细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。 本发明提供具有翻译成分的脊椎动物细胞，例如正交氨基-tRNA合成酶（O-RS）和正交tRNA（O-tRNA）及其各自成分的对，其用于脊椎动物蛋白质生物合成机制以掺入非天然氨基 在生长的多肽链中，在脊椎动物细胞中。