公开(公告)号：US20200318155A1

公开(公告)日：2020-10-08

申请号：US16304035

申请日：2017-05-23

申请人： DENKA SEIKEN CO., LTD.

发明人： Noriyuki SATOH ,  Makoto IKAIDA ,  Yuhko HIRAO ,  Yasuki ITOH

IPC分类号： C12Q1/44 ,  G01N33/92 ,  C12Q1/60 ,  C12Q1/26

摘要： Disclosed are a method and reagent of quantifying cholesterol in triglyceride-rich lipoprotein (TRL-C) in a test sample in a more specific manner without requiring laborious operations. The method of quantifying cholesterol in triglyceride-rich lipoprotein (TRL-C) includes the steps of: (1) selectively eliminating cholesterol in lipoproteins other than triglyceride-rich lipoprotein (TRL) by allowing a cholesterol esterase having a molecular weight of more than 50 kDa and a surfactant(s) to act; and (2) quantifying the remaining TRL-C.

公开(公告)号：US10018641B2

公开(公告)日：2018-07-10

申请号：US14374798

申请日：2013-01-24

申请人： DENKA SEIKEN CO., LTD.

发明人： Noriyuki Sato ,  Yuhko Hirao ,  Yasuki Itoh

IPC分类号： G01N33/92 ,  C12Q1/60 ,  G01N33/00

CPC分类号： G01N33/92 ,  C12Q1/60

摘要： Disclosed is the provision of a method for quantifying HDL3 in a test sample without requiring a laborious operation. The method for quantifying cholesterol in high-density lipoprotein 3 comprises allowing a surfactant(s) which specifically react(s) with a high-density lipoprotein 3 to react with a test sample and quantifying cholesterol, and the surfactant(s) is(are) at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether.

公开(公告)号：US20180120324A1

公开(公告)日：2018-05-03

申请号：US15567903

申请日：2016-04-21

申请人： Masanori Hara ,  Denka Company Limited ,  Denka Seiken Co., Ltd.

发明人： Masanori Hara ,  Hiroyuki Kurosawa ,  Yoshiaki Hirayama

IPC分类号： G01N33/68

摘要： The present invention relates to a method of estimating the number of urinary podocytes, including detecting podocalyxin in a urinary sediment sample liquid, and more specifically, to a method of estimating the number of urinary podocytes, including the following steps (1) to (3): (1) a step of preparing the urinary sediment sample liquid by separating urinary sediment from urine collected from a test subject and solubilizing podocalyxin in the urinary sediment; (2) a step of calculating a podocalyxin excretion amount in the urinary sediment sample liquid through detection of podocalyxin in the urinary sediment sample liquid; and (3) a step of calculating the number of urinary podocytes by dividing the podocalyxin excretion amount in the urinary sediment sample liquid by a podocalyxin amount per podocyte.

公开(公告)号：US20170108490A1

公开(公告)日：2017-04-20

申请号：US15393533

申请日：2016-12-29

申请人： DENKA SEIKEN CO., LTD.

发明人： Hiroyuki Kurosawa ,  Yoshiaki Hirayama

IPC分类号： G01N33/53 ,  G01N33/68

CPC分类号： G01N33/5306 ,  G01N33/6893 ,  G01N2333/705

摘要： An object of the present invention is to provide a method for increasing the sensitivity of an immunoassay system. In order to achieve the object, the present inventors have discovered that the sensitivity of an immunoassay system can be increased by pretreating urine and thus have completed the present invention.

公开(公告)号：US20160161512A1

公开(公告)日：2016-06-09

申请号：US14906589

申请日：2014-07-24

申请人： DENKA SEIKEN CO., LTD.

发明人： Norijuki SATO ,  Yasuki ITOH

IPC分类号： G01N33/92 ,  C07C43/215 ,  C07C43/20

CPC分类号： G01N33/92 ,  C07C43/20 ,  C07C43/215 ,  C12Q1/60 ,  G01N2405/00

摘要： Disclosed are a method and a reagent for quantifying HDL3 in a test sample without requiring laborious operations. The method for quantifying cholesterol in high-density lipoprotein 3 comprises reacting a test sample with one or more surfactants which react specifically with high-density lipoprotein 3, and quantifying cholesterol. When one surfactant is used, the surfactant is one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers having an HLB of 12.5 to 15. When two or more surfactants are used, at least one of the surfactants is at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers, and the two or more surfactants are combined so as to provide the total HLB of 12.5 to 15 of the combined surfactants.

摘要翻译： 公开了一种用于在测试样品中量化HDL3的方法和试剂，而不需要费力的操作。 用于定量高密度脂蛋白3中的胆固醇的方法包括使测试样品与一种或多种与高密度脂蛋白3特异性反应的表面活性剂反应并定量胆固醇。 当使用一种表面活性剂时，表面活性剂选自HLB为12.5〜15的聚氧乙烯多环苯基醚。当使用两种或更多种表面活性剂时，表面活性剂中的至少一种为选自以下的至少一种： 由聚氧乙烯多环苯基醚组成，并且将两种或更多种表面活性剂组合以提供12.5至15的组合表面活性剂的总HLB。

公开(公告)号：US09145578B2

公开(公告)日：2015-09-29

申请号：US13503924

申请日：2010-10-26

申请人： Yasuki Itoh ,  Hitoshi Chiba

发明人： Yasuki Itoh ,  Hitoshi Chiba

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60

摘要： This invention provides a method for separately or simultaneously quantifying cholesterol in a total amount of HDL-C, cholesterol in an HDL subfraction of apoE-containing HDL-C, and cholesterol in an HDL subfraction of apoE-deficient HDL-C. The method comprises enzymatically and separately quantifying cholesterol in apoE-containing HDL and cholesterol in apoE-deficient HDL by adding a surfactant selected from the group consisting of a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 0.7 to less than 1.3, a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of less than 0.7, and a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 1.3 or more to a test sample, allowing cholesterol esterase and cholesterol oxidase to react therewith, and quantifying the hydrogen peroxide generated.

摘要翻译： 本发明提供了一种在apoE缺陷型HDL-C的HDL亚型分离中，分别或同时定量HDL-C，HDL-C，HDL-C的HDL亚组分中的胆固醇和胆固醇的胆固醇的方法。 该方法包括通过添加选自具有含apoE的HDL应答率/ apoE缺陷HDL应答率为0.7的表面活性剂的表面活性剂来酶促分别定量含有apoE的HDL和胆固醇中的胆固醇的apoE缺陷型HDL中的胆固醇 低于1.3的具有apoE的HDL应答率/ apoE缺陷型HDL响应率小于0.7的表面活性剂和具有apoE的HDL应答率/ apoE缺陷型HDL应答率的表面活性剂为1.3或 更多的是测试样品，允许胆固醇酯酶和胆固醇氧化酶与其反应，并量化产生的过氧化氢。

公开(公告)号：US20150044774A1

公开(公告)日：2015-02-12

申请号：US14374798

申请日：2013-01-24

申请人： DENKA SEIKEN CO., LTD.

发明人： Noriyuki Sato ,  Yuhko Hirao ,  Yasuki Itoh

IPC分类号： G01N33/92

CPC分类号： G01N33/92 ,  C12Q1/60

摘要： Disclosed is the provision of a method for quantifying HDL3 in a test sample without requiring a laborious operation. The method for quantifying cholesterol in high-density lipoprotein 3 comprises allowing a surfactant(s) which specifically react(s) with a high-density lipoprotein 3 to react with a test sample and quantifying cholesterol, and the surfactant(s) is(are) at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether.

摘要翻译： 公开了在测试样品中量化HDL3的方法，而不需要费力的操作。 用于定量高密度脂蛋白3中的胆固醇的方法包括使与高密度脂蛋白3特异性反应的表面活性剂与测试样品反应并定量胆固醇，并且表面活性剂是 ）选自聚氧乙烯多环苯基醚和聚氧乙烯苯乙烯化苯基醚中的至少一种。

公开(公告)号：US08906638B2

公开(公告)日：2014-12-09

申请号：US13884475

申请日：2011-11-09

申请人： Yuhko Hirao

发明人： Yuhko Hirao

IPC分类号： C12Q1/60 ,  G01N33/92 ,  C12Q1/44

CPC分类号： C12Q1/60 ,  C12Q1/44 ,  G01N33/92 ,  G01N2333/918

摘要： A method for quantifying remnant-like lipoprotein cholesterol in a sample simply and accurately without requiring separation operations, and a kit therefor are disclosed. A method for quantifying cholesterol in a remnant-like lipoprotein in a sample containing different lipoproteins including the remnant-like lipoprotein comprises a step (1) of erasing cholesterol in lipoproteins other than the remnant-like lipoprotein; and a step (2) of quantifying cholesterol in the remaining remnant-like lipoprotein. The step (1) is carried out under an action of a cholesterol esterase having a molecular weight of more than 40 kDa and not having a subunit having a molecular weight of not more than 40 kDa; and the step (2) is carried out under an action of a cholesterol esterase having a molecular weight of not more than 40 kDa or a cholesterol esterase having a subunit having a molecular weight of not more than 40 kDa.

摘要翻译： 公开了一种用于在不需要分离操作的情况下简单准确地量化样品中的残留样脂蛋白胆固醇的方法，以及用于其的试剂盒。 在含有残留样脂蛋白的不同脂蛋白的样品中，用于定量残留样脂蛋白中的胆固醇的方法包括除残留样脂蛋白以外的脂蛋白中除去胆固醇的步骤（1） 以及量化残留的类似脂蛋白中的胆固醇的步骤（2）。 步骤（1）在分子量大于40kDa且不具有分子量不超过40kDa的亚单位的胆固醇酯酶的作用下进行; 并且步骤（2）在分子量不超过40kDa的胆固醇酯酶或具有分子量不超过40kDa的亚单位的胆固醇酯酶的作用下进行。

公开(公告)号：US20140349277A1

公开(公告)日：2014-11-27

申请号：US14344784

申请日：2012-09-13

申请人： Motohiro Miki

发明人： Motohiro Miki

IPC分类号： G01N33/569 ,  C07K16/10

CPC分类号： G01N33/56983 ,  C07K16/10 ,  C07K2317/34 ,  C07K2317/55 ,  G01N2333/08

摘要： Provided is an anti-human-norovirus GII antibody which responds to substantially all genotypes of the human noroviruses belonging to GII and which can comprehensively detect such human noroviruses GII.The anti-human-norovirus GII antibody that binds to at least one of epitopes which are contained in amino acid regions represented by the following formulas (1) and (2): P-X1-X2-P-G-E  (1) X3-X4-X5-F-Y-X6-L-X7-P-X8  (2) (wherein, X1 represents L, V, N, T, S, M, or R; X2 represents F, Y, or M; X3 represents V or G; X4 represents N or S; X5 represents Q, P, or S; X6 represents S, T, or I; X7 represents A or S; and X8 represents M or V), and of an epitope formed of amino acid 483 of the amino acid sequence represented by SEQ ID NO: 1, or an epitope formed of an amino acid corresponding to amino acid 483, the regions and the amino acids being present in the P domain of a capsid structural protein of a human norovirus GII.

摘要翻译： 提供了抗人诺诺病毒GII抗体，其对属于GII的人类诺维病毒的基本上所有基因型都有反应，并且可以全面检测这种人类诺维病毒GII。 P-X1-X2-PGE（1）X3-X4-表示的氨基酸区域中的至少一个表位的抗人 - 诺如病毒GII抗体， X5-FY-X6-L-X7-P-X8（2）（其中，X1表示L，V，N，T，S，M或R; X2表示F，Y或M; X3表示V或G ; X4表示N或S; X5表示Q，P或S; X6表示S，T或I; X7表示A或S; X8表示M或V），以及由氨基酸483形成的表位 由SEQ ID NO：1表示的氨基酸序列或由对应于氨基酸483的氨基酸形成的表位，所述区域和氨基酸存在于人诺如病毒GII的衣壳结构蛋白的P结构域中。

公开(公告)号：US08703430B2

公开(公告)日：2014-04-22

申请号：US13093984

申请日：2011-04-26

申请人： Shinya Ogasawara ,  Shuhei Miura ,  Akihiko Saito ,  Tetsuro Takeda

发明人： Shinya Ogasawara ,  Shuhei Miura ,  Akihiko Saito ,  Tetsuro Takeda

IPC分类号： G01N33/53

CPC分类号： G01N33/92 ,  C07K16/28 ,  G01N33/6893 ,  G01N2800/00 ,  G01N2800/347

摘要： This invention provides a method for measuring human megalin that can be performed in a simpler manner within a shorter period of time than is possible with conventional techniques, and that can also quantify human megalin. This invention also provides a method that enables diagnosis of functional diseases, which are specific to cells, tissues, or organs, in a site-directed manner at an early stage. Measurement of human megalin enables detection of a disease in an organ in which megalin expression is observed.

摘要翻译： 本发明提供了一种用于测量人类巨蛋白的方法，其可以在比常规技术可能的更短的时间内以更简单的方式进行，并且还可以定量人巨蛋白。 本发明还提供了能够以早期阶段以现场方式诊断功能性疾病的方法，所述功能性疾病特异于细胞，组织或器官。 人巨蛋白的测定能够检测观察到巨蛋白表达的器官中的疾病。