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    Method for genome editing using a self-inactivating CRISPR nuclease
    5.
    发明授权
    Method for genome editing using a self-inactivating CRISPR nuclease 有权

    公开(公告)号：US11739322B2

    公开(公告)日：2023-08-29

    申请号：US16966538

    申请日：2019-01-31

    申请人： INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES

    发明人： Caixia Gao ,  Huawei Zhang ,  Shuai Jin

    IPC分类号： C12N15/64 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90

    CPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/902 ,  C12N2310/20 ,  C12N2510/02 ,  C12N2800/80

    摘要： Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types. The system includes an expression construct including a coding sequence of a gRNA targeting at least one genomic target sequence; an expression construct including a coding sequence of a CRISPR nuclease; and an expression construct including a coding sequence for a gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease. Upon introduction into the cell, the gRNA targeting the at least one genomic target sequence directs the CRISPR nuclease to the at least one genomic target sequence and results in one or more mutations in the genomic target sequence, and the gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease directs the CRISPR nuclease to the target sequence within the coding sequence of the CRISPR nuclease and results in an inactivating mutation of the CRISPR nuclease.

    Wheat plants resistant to powdery mildew
    9.
    发明授权
    Wheat plants resistant to powdery mildew 有权

    公开(公告)号：US10988775B2

    公开(公告)日：2021-04-27

    申请号：US15745479

    申请日：2016-07-15

    申请人： Institute of Genetics and Developmental Biology Chinese Academy of Sciences

    发明人： Caixia Gao ,  Jin-Long Qiu ,  Yanpeng Wang

    IPC分类号： A01H1/00 ,  A01H5/10 ,  C12N15/82 ,  A01H6/46

    摘要： In one aspect, this disclosure relates to a wheat plant, plant part or plant cell that has increased resistance to powdery mildew, wherein said plant comprises a loss of function mutation in the coding regions of two alleles selected from TaMLO-A1, TaMLO-B1 and TaMLO-D1 and reduced expression of the third TaMLO allele. In another aspect, this disclosure provides a method for producing a wheat plant, plant part or plant cell with increased resistance to powdery mildew, wherein the method comprises using targeted genome modification comprising introducing a loss of function mutation into the coding regions of two MLO alleles selected from TaMLO-A1, TaMLO-B1 and TaMLO-D1 and decreasing expression of the third TaMLO allele.