公开(公告)号：US09346864B2

公开(公告)日：2016-05-24

申请号：US13985800

申请日：2012-02-15

申请人： Thorsten Luehrs ,  Felix Deluweit

发明人： Thorsten Luehrs ,  Felix Deluweit

IPC分类号： G01N33/00 ,  C07K14/47 ,  B01F7/00 ,  B01F11/02 ,  B01F13/08 ,  B01F15/00

CPC分类号： B01F11/0225 ,  B01F7/00008 ,  B01F7/00541 ,  B01F7/008 ,  B01F11/0258 ,  B01F13/0836 ,  B01F13/0845 ,  B01F15/00006 ,  B01F15/00389 ,  B01F15/00707 ,  B01F15/0072 ,  B01F2215/0073 ,  B01F2215/0427 ,  B01F2215/0431 ,  B01F2215/0454 ,  B01F2215/0477 ,  B01F2215/0481 ,  C07K14/47

摘要： The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

摘要翻译： 本发明提供了通过使天然构象朊病毒蛋白与聚集的构象朊病毒蛋白在液体制剂中接触而制备具有聚集构象的朊病毒蛋白的方法，并且对其进行至少一个循环或多个施加剪切力以循环的循环 朊病毒蛋白的聚集体，其中施加的剪切力被精确地控制。 本发明涉及通过扩增方法获得的聚合状态朊病毒蛋白，该聚合状态朊病毒蛋白具有一个构象。 在一批内相同，并且在批次之间可再现。 在Western印迹中可通过蛋白酶抗性检测。

公开(公告)号：US10900976B2

公开(公告)日：2021-01-26

申请号：US15320654

申请日：2015-07-01

申请人： SeNostic GmbH

发明人： Thorsten Luehrs

IPC分类号： G01N33/68 ,  G16B20/00 ,  G16B99/00 ,  G01N1/04 ,  G01N1/28 ,  G01N21/64

摘要： The invention provides an analytical process for analysing the presence of at least one aggregated conformation prion protein in a sample of body fluid or a sample of tissue and uses the dependency of the amplification of the aggregated conformation on the shear-force intensity applied to the native conformation prion protein, which is also dependent on the specific seed present in the admixture with native conformation prion protein, for specifically analysing for the presence of an aggregated conformation prion protein in the sample. The process of the invention contains the step of determining the content of aggregated conformation prion protein generated in admixture with the sample to be analysed using one shear-force intensity, preferably using least at two different shear-force intensities and the step of comparing data on these contents of generated prion protein having an aggregated conformation with data on the content of aggregated prion protein that is pre-determined, each at the same shear-force intensity for a mixture of the same native conformation prion protein with a reference sample as a seed.