公开(公告)号：US20240280564A1

公开(公告)日：2024-08-22

申请号：US18566782

申请日：2022-06-03

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads ,  The Regents of The University of California

发明人： Katerina Akassoglou ,  Mark Petersen ,  Anke Meyer-Franke

IPC分类号： G01N33/50 ,  C12N5/00 ,  C12N5/079

CPC分类号： G01N33/5058 ,  C12N5/0018 ,  C12N5/0622 ,  G01N33/5026 ,  G01N33/5073 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2506/08 ,  G01N2500/10

摘要： A high-throughput, high-content assay to screen for an agent which overcomes remyelination inhibition by an extrinsic inhibitor.

公开(公告)号：US20240209381A1

公开(公告)日：2024-06-27

申请号：US18393522

申请日：2023-12-21

申请人： The J. David Gladstone Institutes

发明人： Taha Yasin Taha ,  Melanie Ott ,  Takako Tabata

IPC分类号： C12N15/66

CPC分类号： C12N15/66 ,  C12N2770/20043

摘要： Provided herein is a novel cloning system with rational fragment design and single-pot ligation (pGLUE) that allows systematic exchange and mutagenesis of genes and rapid construction of entire molecular clones and replicons of virus within days.

公开(公告)号：US12016934B2

公开(公告)日：2024-06-25

申请号：US17552464

申请日：2021-12-16

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone

发明人： Katerina Akassoglou ,  Warner Greene ,  Jae Kyu Ryu ,  Mauricio Montano

IPC分类号： A61K49/00 ,  A01K67/00 ,  G01N33/50

CPC分类号： A61K49/0008 ,  A01K67/00 ,  G01N33/5088 ,  A01K2227/105 ,  A01K2267/0337 ,  G01N2333/165

摘要： Described herein is an animal model useful for identifying therapeutic agents that can inhibit the physiological effects or symptoms of COVID-19 infection, including the effects of the following on one or more organs of the animal: inflammation, oxidative stress, fibrin deposition, blood brain barrier breakdown, clotting, and vascular problems.

公开(公告)号：US20240034775A1

公开(公告)日：2024-02-01

申请号：US17923456

申请日：2021-05-05

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads ,  Annexon, Inc.

发明人： Jeanne T. Paz ,  Sethu Sankaranarayanan ,  Ted Yednock

IPC分类号： C07K16/18 ,  G01N33/68 ,  A61P25/08

CPC分类号： C07K16/18 ,  G01N33/6896 ,  A61P25/08 ,  G01N2800/2857 ,  G01N2333/4716 ,  A61K2039/505

摘要： The present disclosure relates generally to methods of preventing, reducing risk of developing, or treating epilepsy, comprising administering to a subject an inhibitor of the classical complement pathway.

公开(公告)号：US11807852B2

公开(公告)日：2023-11-07

申请号：US17380773

申请日：2021-07-20

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone

发明人： Melanie Ott ,  Daniela Boehm

IPC分类号： C12N15/113 ,  A61K38/15 ,  A61K45/06 ,  A61K31/19 ,  A61K31/22 ,  A61K31/365 ,  A61K31/551 ,  A61K31/713 ,  A61K31/235 ,  A61K31/496 ,  A61K31/538 ,  A61K31/167 ,  A61K31/222

CPC分类号： C12N15/1137 ,  A61K31/167 ,  A61K31/19 ,  A61K31/22 ,  A61K31/222 ,  A61K31/235 ,  A61K31/365 ,  A61K31/496 ,  A61K31/538 ,  A61K31/551 ,  A61K31/713 ,  A61K38/15 ,  A61K45/06 ,  C12N2310/14 ,  C12N2310/531 ,  A61K31/19 ,  A61K2300/00 ,  A61K31/551 ,  A61K2300/00 ,  A61K38/15 ,  A61K2300/00

摘要： The present disclosure provides compositions and methods for reactivating latent immunodeficiency virus and/or reducing transcription of HIV integrated into the genome of an HIV-infected cell. The present disclosure provides compositions and methods for treating an immunodeficiency virus infection.

公开(公告)号：US11584931B2

公开(公告)日：2023-02-21

申请号：US16467233

申请日：2017-12-14

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone ,  The Regents of the University of California

发明人： Leor S. Weinberger ,  Timothy J. Notton

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12N15/66 ,  C12N15/867

摘要： Provided are methods and compositions for generating a deletion library, and methods and compositions for generating and identifying a defective interfering particle (DIP). Also provided are transposon cassettes. A subject method can include: inserting a transposon cassette comprising a target sequence for a sequence specific DNA endonuclease into a population of circular target DNAs to generate a population of transposon-inserted circular target DNAs; contacting the population of transposon-inserted circular target DNAs with the sequence specific DNA endonuclease to generate a population of cleaved linear target DNAs; contacting the population of cleaved linear target DNAs with one or more exonucleases to generate a population of deletion DNAs; and circularizing the deletion DNAs to generate a library of circularized deletion DNAs. The population of circular target DNAs can include viral genomic DNA. Also provided are human immunodeficiency virus (HIV) deletion mutants, e.g., interfering, conditionally replicating, HIV deletion mutants, and related constructs.

公开(公告)号：US11573222B2

公开(公告)日：2023-02-07

申请号：US16572365

申请日：2019-09-16

申请人： The J. David Gladstone Institutes

发明人： Katerina Akassoglou ,  Jae Kyu Ryu ,  Anke Meyer-Franke

IPC分类号： G01N33/50

摘要： The present invention provides compositions exhibiting in vivo activity of fibrin in an in vitro setting, in vitro assays comprising such compositions, methods of producing such compositions, and methods of using such compositions and assays. The compositions of the invention include molecules with the biochemical properties of 1) high affinity binding to fibrin receptors and 2) activation of cell-signaling systems comparable to that observed in vivo by fibrin. The fibrin compositions of the invention are compatible both in biochemical assays and cell-based assays, and thus useful for in vitro assays for screening of test agents that modulate cell activation and/or signaling pathways mediated by fibrin or associated with fibrin activity.

公开(公告)号：US20220348638A1

公开(公告)日：2022-11-03

申请号：US17763424

申请日：2020-09-24

申请人： The J. David Gladstone Institutes, a testamentary trust estabilished under the Will of J. David Glad

发明人： Yadong Huang ,  Kelly Zalocusky

IPC分类号： C07K14/775 ,  A61P25/28

摘要： A method for reducing neuronal and synaptic degeneration or loss in a population of neuronal cells is provided as well as a method of treating an individual with a neurocognitive disorder. Aspects of the methods include modulating the level and/or activity of apolipoprotein E (apoE) in a population of neuronal cells where the modulating reduces the level and/or activity of an MHC pathway polypeptide in the population of neuronal cells.

公开(公告)号：US20220307007A1

公开(公告)日：2022-09-29

申请号：US17639043

申请日：2020-09-11

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads ,  President and Fellows of Harvard College

发明人： Seth Shipman

IPC分类号： C12N15/10 ,  C12N15/63 ,  C12N9/12 ,  C12Q1/48 ,  C12N9/22

摘要： Engineered retrons, modified to enhance production of multicopy single-stranded DNA (msDNA), are provided. In addition, vector systems encoding such engineered retrons and methods of using engineered retrons and vector systems encoding them in various applications such as CRISPR/Cas-mediated genome editing, recombineering, cellular barcoding, and molecular recording are also disclosed.

公开(公告)号：US20220187280A1

公开(公告)日：2022-06-16

申请号：US17685674

申请日：2022-03-03

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads

发明人： Jeremy Linsley ,  Kevan Shah ,  Steven M. Finkbeiner

IPC分类号： G01N33/50 ,  G01N33/84 ,  C12N15/85

摘要： Genetically encoded death indicator (GEDI) polypeptides and nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides to monitor cell death events in vitro and in vivo, particularly in neuronal cell death, are also provided.