公开(公告)号：US06936462B1

公开(公告)日：2005-08-30

申请号：US09719236

申请日：1999-06-14

申请人： David Geraint Owen ,  Nicholas Gerard Byrne

发明人： David Geraint Owen ,  Nicholas Gerard Byrne

IPC分类号： G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  G01N37/00 ,  C12M1/34 ,  C12M3/00

CPC分类号： G01N33/502 ,  B01L3/5025 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0893 ,  B01L2300/16 ,  G01N27/28 ,  G01N33/48728 ,  G01N33/5438 ,  G01N33/6872 ,  G01N2500/04 ,  G01N2500/10 ,  Y10T29/49826

摘要： The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.

公开(公告)号：US07384733B1

公开(公告)日：2008-06-10

申请号：US09857456

申请日：1999-12-06

申请人： Nicholas Gerard Byrne ,  David Geraint Owen

发明人： Nicholas Gerard Byrne ,  David Geraint Owen

IPC分类号： C12Q1/00 ,  C12Q1/02 ,  C12M1/34 ,  C12M3/00

CPC分类号： G01N33/48728 ,  C12M41/46

摘要： The invention provides a novel development of the conventional patch clamp technique for measurement of whole cell electrical activity. The invention provides for one or more cell or cells to be suspended in a liquid medium at a liquid/air interface (by virtue of the effect of surface tension at the interface) whereby the cell or cells are accessible at the interface to a microstructure electrode (such as a pipette tip) to which a cell can attach to form an electrical seal, for the purpose of whole cell voltage clamp recording. According to the invention the electrode can be caused to form a high resistance electrical seal with a cell suspended in the liquid at the liquid/air interface without the need to press the cell against a solid support surface. The invention also provides apparatus for carrying out the interface patch clamp technique and control logic for operating a computer to carry out the interface patch clamp technique.

摘要翻译： 本发明提供了用于测量全细胞电活动的常规膜片钳技术的新发展。 本发明提供一种或多种在液体/空气界面处悬浮在液体介质中的细胞或细胞（借助于界面处的表面张力的作用），由此在与微结构电极的界面处可接触细胞或细胞 （例如移液管尖端），电池可以附接到其上以形成电气密封，用于全电池电压钳记录的目的。 根据本发明，可以使电极与在液体/空气界面处悬浮在液体中的电池形成高电阻电气密封，而不需要将电池压在固体支撑表面上。 本发明还提供了用于执行界面膜片钳技术的装置和用于操作计算机以执行界面膜片钳技术的控制逻辑。