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    Genomic and proteomic approaches for the development of cell culture medium
    1.
    发明申请
    Genomic and proteomic approaches for the development of cell culture medium 审中-公开
    用于细胞培养基发育的基因组和蛋白质组学方法

    公开(公告)号：US20040265880A1

    公开(公告)日：2004-12-30

    申请号：US10842649

    申请日：2004-05-10

    申请人： Sigma-Aldrich Co.

    发明人： Laurel M. Donahue ,  Daniel W. Allison ,  Terrell K. Johnson ,  Kathryn A. Aboytes ,  Danny Fong

    IPC分类号： C12Q001/68 ,  C12N005/00

    CPC分类号： C12Q1/6837 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  C12N5/0018 ,  C12N5/0037

    摘要： The present invention relates to a method for designing a cell culture medium adapted to support a cell line in a pre-defined manner. The method includes generating an expression profile of a cell line, identifying from the expression profile a set of biomolecules to evaluate for their effect on an end-point assay using the cell line, testing each biomolecule in the set for its effect in the endpoint assay, wherein each biomolecule that is determined to have a measurable effect in the end-point assay relative to a control, not containing the biomolecule, is considered a positive biomolecule and formulating a cell culture medium for the cell line by adding a positive biomolecule to a basal medium to form a modified medium, and determining whether the modified medium is sufficient to support the cell line in the pre-defined manner. Methods for identifying biomolecules for use in designing a cell culture medium adapted to support a cell line in a pre-defined manner, methods for preparing a serum-free cell culture medium that is sufficient to support a cell line and arrays for use in such methods are also provided.

    摘要翻译： 本发明涉及一种用于以预定方式支持细胞系的细胞培养基的设计方法。 该方法包括产生细胞系的表达谱，从表达谱中鉴定一组生物分子，以评估其对使用细胞系的终点测定的影响，测试该组中的每个生物分子在终点测定中的作用 其中，相对于不包含生物分子的对照，在终点测定中确定具有可测量效应的每个生物分子被认为是阳性生物分子，并且通过向阳性生物分子添加阳性生物分子来配制细胞系的细胞培养基 基础培养基以形成修饰的培养基，并且确定修饰的培养基是否足以以预先确定的方式支持细胞系。 用于鉴定用于设计适于以预定方式支持细胞系的细胞培养基的生物分子的方法，用于制备足以支持细胞系的无血清细胞培养基的方法和用于该方法的阵列 也提供。

    Egg freezing and storing tool and method
    2.
    发明申请
    Egg freezing and storing tool and method 有权
    蛋冻存储工具及方法

    公开(公告)号：US20040259072A1

    公开(公告)日：2004-12-23

    申请号：US10824605

    申请日：2004-04-15

    申请人： Kabushiki Kaisha KITAZATO SUPPLY

    发明人： Masashige Kuwayama ,  Futoshi Inoue

    IPC分类号： A01N001/00 ,  A01N001/02 ,  C12N005/00 ,  C12N005/08 ,  C12N005/02 ,  F25B039/02

    CPC分类号： A01N1/02 ,  A01N1/0268 ,  A61D19/022

    摘要： An egg freezing and storing instrument has an egg freezing and storing tube made of a liquid nitrogen-resistant material; and a metal cylindrical protection member for protecting the tube. The tube has a body part; and an egg-storing small-diameter part having an inner diameter of 0.1 mm to 0.5 mm. The tube can be heat-sealed at a front side of the small-diameter part and at a rear side of the body part. The cylindrical protection member has a tubular part for accommodating a front side of the small-diameter part of the tube; and a semi-tubular part for accommodating a portion of the small-diameter part not accommodated in the tubular part and a front portion of the body part.

    摘要翻译： 鸡蛋冷冻储存器具有一个由防液体材料制成的鸡蛋冷冻储存管; 以及用于保护管的金属圆柱形保护构件。 管具有身体部分; 以及内径为0.1mm〜0.5mm的储蛋小口径部。 管可以在小直径部分的前侧和身体部分的后侧被热密封。 圆柱形保护构件具有用于容纳管的小直径部分的前侧的管状部分; 以及用于容纳未容纳在管状部分中的小直径部分的一部分和身体部分的前部的半管状部分。

    HIV therapeutic
    3.
    发明申请
    HIV therapeutic 审中-公开
    艾滋病治疗

    公开(公告)号：US20040248296A1

    公开(公告)日：2004-12-09

    申请号：US10393411

    申请日：2003-03-20

    发明人： Paul J. Beresford ,  Judy Lieberman ,  Michael F. Murray ,  Carl D. Novina ,  Phillip A. Sharp

    IPC分类号： A61K048/00 ,  C12N005/00

    CPC分类号： C12N15/1132 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12Q1/703

    摘要： The present invention provides siRNA methods and compositions for inhibiting HIV infection and/or replication, as well as systems for identifying effective siRNAs for inhibiting HIV and systems for studying HIV infective mechanisms. The invention also provides methods and compositions for inhibiting infection, pathogenicity and/or replication of an infectious agent; for example, by using siRNAs to inhibit host cell gene expression.

    摘要翻译： 本发明提供用于抑制HIV感染和/或复制的siRNA方法和组合物，以及鉴定用于抑制HIV的有效siRNA和用于研究HIV感染机制的系统的系统。 本发明还提供用于抑制感染剂的感染，致病性和/或复制的方法和组合物; 例如通过使用siRNA来抑制宿主细胞基因表达。

    Selectable marker for genetically engineered cells and tissues
    8.
    发明申请
    Selectable marker for genetically engineered cells and tissues 审中-公开
    用于基因工程细胞和组织的可选标记

    公开(公告)号：US20040115800A1

    公开(公告)日：2004-06-17

    申请号：US10466705

    申请日：2003-12-15

    发明人： Richard L Momparler ,  Jacques Galipeau ,  Nicoletta Eliopoulus

    IPC分类号： C07H021/04 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74 ,  C12N005/00 ,  C12N005/02 ,  C12N005/08

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2840/203

    摘要： The present invention relates to selectable marker gene, method of producing genetically modified cells allowing expression of a bicistronic vector, and ex vivo gene therapy applications. The invention also relates to selection of genetically engineered cells from the mixed population, prior to implantation in vivo. The invention describes the human cytidine deaminase (CD) gene as an ex vivo dominant selectable marker in gene-modified primary cells with cytosine nucleoside analogues. A bicistronic retrovector comprising the human CD coding sequence and the enhanced green fluorescent protein (GFP) reporter gene is used for the transduction of cells. The invention describes the introduction of CD gene in cell and gene therapy applications for the ex vivo dominant selection of genetically engineered cells.

    摘要翻译： 本发明涉及选择性标记基因，生产允许双顺反子载体表达的遗传修饰细胞的方法和离体基因治疗应用。 本发明还涉及在体内植入之前从混合群体中选择遗传工程改造的细胞。 本发明描述了人胞苷脱氨酶（CD）基因作为具有胞嘧啶核苷类似物的基因修饰的原代细胞中的离体显性选择标记。 包含人CD编码序列和增强型绿色荧光蛋白（GFP）报道基因的双顺反子载体用于细胞转导。 本发明描述了CD基因在细胞和基因治疗应用中的引入，用于遗传工程细胞的离体显性选择。

    Insect cell primary culture medium, extracellular matrix, and process of preparing an insect culture cell line in a short period of time using the medium and matrix
    10.
    发明申请
    Insect cell primary culture medium, extracellular matrix, and process of preparing an insect culture cell line in a short period of time using the medium and matrix 失效
    昆虫细胞原代培养基，细胞外基质和使用培养基和基质在短时间内制备昆虫培养细胞系的方法

    公开(公告)号：US20040101961A1

    公开(公告)日：2004-05-27

    申请号：US10715361

    申请日：2003-11-19

    申请人： National Institute of Agrobiological

    发明人： Shigeo Imanishi ,  Atsunobu Haga ,  Jun Mitsuhashi

    IPC分类号： C12N005/00 ,  C08B037/08

    CPC分类号： C12N5/0601 ,  A61K2039/55572 ,  C12N2500/70

    摘要： A novel cell culture medium suitable for primary culture of insect cells, an insect-derived water-soluble chitin, and a process of preparing an insect culture cell line in a short period of time by using the insect primary culture medium and the insect-derived water-soluble chitin. The insect cell primary culture medium comprises lactalbumin hydrolysate, yeastolate, and tryptose phosphate broth as protein extracts, and polyvinylpyrrolidone as a viscosity-supplementing agent. The insect-derived water-soluble chitin is subjected to deacetylation as the sole chemical modification.

    摘要翻译： 适用于昆虫细胞原代培养的新型细胞培养基，昆虫来源的水溶性几丁质，以及通过使用昆虫原代培养基和昆虫来源于昆虫的水溶性几丁质，在短时间内制备昆虫培养细胞系的方法 水溶性几丁质。 昆虫细胞原代培养基包括作为蛋白质提取物的乳清蛋白水解物，酵母淀粉和柠檬酸胰蛋白肉汤，以及作为粘度补充剂的聚乙烯吡咯烷酮。 作为唯一的化学修饰，将来自昆虫的水溶性几丁质作为去乙酰化。