公开(公告)号：US20240002892A1

公开(公告)日：2024-01-04

申请号：US18035281

申请日：2021-09-09

Applicant: SHENZHEN READLINE BIOTECH CO., LTD.

Inventor： Mingru HUANG ,  Junfeng PAN ,  Jian LIU

IPC: C12P13/04 ,  C12N9/10

CPC classification number: C12P13/04 ,  C12N9/13 ,  C12N9/1007 ,  C12Y201/01044 ,  C12Y201/01067 ,  C12Y208/01001

Abstract: A compound enzyme, comprising L-histidine methylase, halogen methyltransferase and trimethylhistidine sulfurylase. L-histidine is catalyzed by L-histidine methylase and halogen methyltransferase to obtain trimethylhistidine, and then is catalyzed by trimethylhistidine sulfurase to obtain the L-ergothioneine. The method can realize conversion from L-histidine to L-ergothioneine only in two steps. Moreover, SAM enzyme is regenerated, such that the usage amount of the SAM enzyme is greatly reduced. Therefore, the method greatly reduces the raw material cost. Moreover, the method is high in reaction concentration (about 30 g/L), simple in process and good in industrial production prospect.