公开(公告)号：US11680279B2

公开(公告)日：2023-06-20

申请号：US16200011

申请日：2018-11-26

Applicant: AJINOMOTO CO., INC.

Inventor： Benjamin Mijts ,  Christine Roche ,  Rekha Murali ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki ,  Keiko Noguchi

IPC: C12P13/12 ,  C12P7/24 ,  C12N9/10 ,  C12N9/06 ,  C12P7/42 ,  C12N1/20 ,  C12R1/15 ,  C12R1/19 ,  C12R1/46

CPC classification number: C12P7/24 ,  C12N1/205 ,  C12N9/0012 ,  C12N9/1014 ,  C12N9/1085 ,  C12P7/42 ,  C12P13/12 ,  C12R2001/15 ,  C12R2001/19 ,  C12R2001/46 ,  C12Y105/0102 ,  C12Y201/02002 ,  C12Y205/01006

Abstract: A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism that is able to produce the objective substance, which microorganism has been modified so that the activity of an enzyme involved in SAM cycle (SAM cycle enzyme) is increased.

公开(公告)号：US20120100617A1

公开(公告)日：2012-04-26

申请号：US13272543

申请日：2011-10-13

Applicant: Juergen Nett

Inventor： Juergen Nett

IPC: C12N15/81 ,  C12N15/52 ,  C12N1/19

CPC classification number: C12N15/815 ,  C12N9/1014 ,  C12N9/1077 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y201/02002 ,  C12Y204/02014 ,  C12Y403/02002 ,  C12Y603/02017 ,  C12Y603/03001 ,  C12Y603/04004 ,  C12Y603/04013 ,  C12Y603/05003

Abstract: Disclosed are the ADE3, ADE4, ADE5, 7, ADE6, ADE8, ADE12, and ADE13 genes encoding various enzymes in the adenine biosynthesis pathway of Pichia pastoris. The loci in the Pichia pastoris genome encoding these enzymes are useful sites for stable integration of heterologous nucleic acid molecules into the Pichia pastoris genome. The genes or gene fragments encoding the particular enzymes, which may be used as selection markers for constructing recombinant Pichia pastoris.

Abstract translation: 公开了在巴斯德毕赤酵母的腺嘌呤生物合成途径中编码各种酶的ADE3，ADE4，ADE5,7，ADE6，ADE8，ADE12和ADE13基因。 编码这些酶的巴斯德毕赤酵母基因组中的基因座是将异源核酸分子稳定整合到巴斯德毕赤酵母基因组中的有用位点。 编码特定酶的基因或基因片段，其可用作构建重组巴斯德毕赤酵母的选择标记。