公开(公告)号：US07445915B2

公开(公告)日：2008-11-04

申请号：US10508313

申请日：2003-03-19

申请人： Masato Ikeda ,  Mikiro Hayashi ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Hiroshi Mizoguchi ,  Satoshi Nakagawa

发明人： Masato Ikeda ,  Mikiro Hayashi ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Hiroshi Mizoguchi ,  Satoshi Nakagawa

IPC分类号： C12N13/04 ,  C12P13/08 ,  C12P13/10 ,  C12P13/14 ,  C12P13/22 ,  C12P13/24 ,  C07H21/04

CPC分类号： C12P13/04 ,  C12N9/88 ,  C12P13/08 ,  C12P13/10 ,  C12P13/14 ,  C12P13/227 ,  C12P13/24

摘要： The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.

摘要翻译： 本发明提供一种多肽，其包含氨基酸序列，其中至少一个氨基酸被缺失，取代或添加在衍生自属于棒状细菌的微生物的异丙基丙酸异丙酯的氨基酸序列中，其中产生多肽的棒状杆菌型细菌包含 作为唯一的异丙基苹果酸异构酶的氨基酸序列在基本培养基中表现出部分亮氨酸要求; 编码该多肽的DNA，包含DNA的重组DNA，用该重组DNA转化的微生物和使用该微生物产生L-氨基酸的方法。