公开(公告)号：US07572632B2

公开(公告)日：2009-08-11

申请号：US10685802

申请日：2003-10-16

申请人： Richard Fike ,  William Whitford ,  William Biddle

发明人： Richard Fike ,  William Whitford ,  William Biddle

IPC分类号： C12N6/00 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

摘要： Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.

摘要翻译： 提供了涉及营养介质，培养基补充剂，培养基亚组和缓冲液制剂的方法和组合物。 还公开了在用溶剂重构时产生特定离子和pH条件的粉末缓冲剂制剂。 还提供了生产这些培养基，培养基，培养基和缓冲液制剂的方法以及用于培养原核和真核细胞（包括人细胞）的试剂盒和方法。 还公开了生产无菌粉末培养基，培养基补充剂，粉末生长因子，培养基亚组和缓冲液制剂的方法。 灭菌方法包括γ照射。 公开了可以使用喷雾干燥细胞悬浮液的干细胞粉末的制造方法。 还公开了通过这些方法生产的细胞，培养基，培养基，培养基和缓冲粉。