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    Dry powder cells and cell culture reagents and methods of production thereof
    1.
    发明授权
    Dry powder cells and cell culture reagents and methods of production thereof 有权
    干粉细胞和细胞培养试剂及其生产方法

    公开(公告)号：US07572632B2

    公开(公告)日：2009-08-11

    申请号：US10685802

    申请日：2003-10-16

    申请人： Richard Fike ,  William Whitford ,  William Biddle

    发明人： Richard Fike ,  William Whitford ,  William Biddle

    IPC分类号： C12N6/00 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00

    CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

    摘要： Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.

    摘要翻译： 提供了涉及营养介质，培养基补充剂，培养基亚组和缓冲液制剂的方法和组合物。 还公开了在用溶剂重构时产生特定离子和pH条件的粉末缓冲剂制剂。 还提供了生产这些培养基，培养基，培养基和缓冲液制剂的方法以及用于培养原核和真核细胞（包括人细胞）的试剂盒和方法。 还公开了生产无菌粉末培养基，培养基补充剂，粉末生长因子，培养基亚组和缓冲液制剂的方法。 灭菌方法包括γ照射。 公开了可以使用喷雾干燥细胞悬浮液的干细胞粉末的制造方法。 还公开了通过这些方法生产的细胞，培养基，培养基，培养基和缓冲粉。

    Dry powder cells and cell culture reagents and methods of production thereof
    4.
    发明授权
    Dry powder cells and cell culture reagents and methods of production thereof 失效
    干粉细胞和细胞培养试剂及其生产方法

    公开(公告)号：US06383810B2

    公开(公告)日：2002-05-07

    申请号：US09023790

    申请日：1998-02-13

    申请人： Richard Fike ,  William Whitford ,  William Biddle

    发明人： Richard Fike ,  William Whitford ,  William Biddle

    IPC分类号： C12N500

    CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

    摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.

    摘要翻译： 本发明一般涉及营养培养基，培养基，培养基和缓冲液制剂。 具体地说，本发明提供粉末营养培养基，中等添加剂和中等亚组分制剂，特别是细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养物质的细胞培养基 促进细胞体外培养的因素。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明特别涉及这些培养基，培养基，培养基和缓冲液制剂的生产方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞的试剂盒和方法（包括 人类细胞），使用这些干粉营养介质，培养基补充剂，培养基亚组和缓冲液制剂。 本发明还涉及生产无菌粉末培养基，培养基补充剂（特别是粉末状血清如粉末状FBS，粉状转铁蛋白，粉末状胰岛素，粉末状器官提取物（如牛脑或垂体提取物）），粉末生长因子（如EGF， FGF等）等），介质亚组和缓冲液制剂。 在特别优选的方面，本发明涉及这样的方法，其中灭菌是通过γ照射来完成的。 本发明还涉及生产干细胞粉末的方法，包括喷雾干燥细胞悬浮液。 本发明还涉及通过这些方法制备的细胞，培养基，培养基，培养基和缓冲粉。

    Dry powder cells and cell culture reagents and methods of production thereof
    5.
    发明申请
    Dry powder cells and cell culture reagents and methods of production thereof 审中-公开

    公开(公告)号：US20060003447A1

    公开(公告)日：2006-01-05

    申请号：US11024051

    申请日：2004-12-29

    申请人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle

    发明人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle

    IPC分类号： C12N5/02

    CPC分类号： C12N5/0018 ,  C12N2500/60

    摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

    Adhering Surfaces
    6.
    发明申请
    Adhering Surfaces 审中-公开
    贴面

    公开(公告)号：US20080299601A1

    公开(公告)日：2008-12-04

    申请号：US12047997

    申请日：2008-03-13

    申请人： Richard Fike ,  Robert Burrier

    发明人： Richard Fike ,  Robert Burrier

    IPC分类号： C12Q1/02 ,  C12N5/06

    CPC分类号： C12N5/0068 ,  C12M23/20 ,  C12N2533/30 ,  C12N2533/74

    摘要： The present invention relates, in part, to compositions useful for cell culture having one surface in adherence to another surface, e.g., a cell culture matrix in adherence to a surface. The present invention also relates, in part, to methods of adhering one surface to another surface, e.g., adhering a cell culture matrix to a surface, and compositions relating to such methods. The present invention also provides in part, methods for adhering a cell to a surface. Related methods are also provided for determining the effect of at least one compound on a cell(s).

    摘要翻译： 本发明部分地涉及可用于细胞培养物的组合物，所述组合物具有粘附于另一表面的一个表面，例如粘附于表面的细胞培养基质。 本发明还部分地涉及将一个表面粘附到另一个表面的方法，例如将细胞培养基质粘附到表面上，以及与这些方法相关的组合物。 本发明还部分提供了将电池附着在表面上的方法。 还提供了相关方法用于确定至少一种化合物对细胞的影响。

    Compositions and methods for stabilizing susceptible compounds
    8.
    发明授权
    Compositions and methods for stabilizing susceptible compounds 审中-公开

    公开(公告)号：US10669522B2

    公开(公告)日：2020-06-02

    申请号：US13984263

    申请日：2012-02-07

    申请人： Richard Fike ,  Bruce Branchaud ,  Shawn Barrett

    发明人： Richard Fike ,  Bruce Branchaud ,  Shawn Barrett

    IPC分类号： C12N5/00

    摘要： Methods for increasing the stability of, or protecting, labile components, such as ethanolamine, growth factors, vitamins, etc., in compositions such as a cell culture medium, are described. Stability of the labile compound is increased either, by derivatization or by sequestering the labile compound. Sequestering can be done either by encapsulation within a microcapsule or by the use of sequestering agents. Encapsulation can provide controlled release of the susceptible compound being protected. These methods may improve or extend the storage conditions, the shipping and handling of media compositions comprising the labile compounds at room temperature rather than at lower temperatures, thereby decreasing shipping costs.

    Dry powder cell culture products containing lipid and methods of production thereof
    10.
    发明申请
    Dry powder cell culture products containing lipid and methods of production thereof 审中-公开

    公开(公告)号：US20060270038A1

    公开(公告)日：2006-11-30

    申请号：US11434513

    申请日：2006-05-16

    申请人： Richard Fike ,  Robert Radominski ,  Barbara Dadey ,  Richard Hassett

    发明人： Richard Fike ,  Robert Radominski ,  Barbara Dadey ,  Richard Hassett

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0018 ,  C12N2500/36 ,  C12N2500/60

    摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations that contain lipid. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors including lipid components that facilitate the in vitro cultivation of cells. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to media, media supplement, media subgroup and buffer powders produced by these methods.