公开(公告)号：US06303350B1

公开(公告)日：2001-10-16

申请号：US09445543

申请日：2000-03-07

申请人： Kazutoh Takesako ,  Mitsuhiro Ueno ,  Naoyuki Awazu ,  Yoko Uno ,  Ikunoshin Kato

发明人： Kazutoh Takesako ,  Mitsuhiro Ueno ,  Naoyuki Awazu ,  Yoko Uno ,  Ikunoshin Kato

IPC分类号： C12P1302

CPC分类号： C07C235/76 ,  C12P13/02 ,  Y10S435/911

摘要： The present invention has for its object to provide novel biologically active substances which is of value as a therapeutic agent for fungal infections and immune disorders. This invention is related to a biologically active substance TKR2449 analog(s) which is represented by the following general formula (A); (In the formula, R1, R2 and R3 are the same or differ each other, and each represents hydrogen or an alkyl group of carbon number of 1 to 4. R4 is a linear or branched alkyl or alkenyl group of carbon number of 1 to 8.).

摘要翻译： 本发明的目的是提供新的生物活性物质，其作为真菌感染和免疫疾病的治疗剂是有价值的。 本发明涉及由以下通式（A）表示的生物活性物质TKR2449类似物：（式中，R 1，R 2和R 3相同或不同，各自表示氢或 碳数为1〜4的烷基.R 4为碳原子数为1〜8的直链或支链烷基或烯基。

公开(公告)号：US06699695B1

公开(公告)日：2004-03-02

申请号：US09622201

申请日：2000-08-14

申请人： Akinobu Matsuyama ,  Masato Kawabe ,  Toru Nagasawa

发明人： Akinobu Matsuyama ,  Masato Kawabe ,  Toru Nagasawa

IPC分类号： C12P1302

CPC分类号： C12R1/365 ,  C12P13/02 ,  Y10S435/822

摘要： A nitrile compound having a complicated structure (e.g., 2-hydroxy-4-methylthiobutyronitrile) is converted into an amide compound with high production efficiency, by using a novel microorganism of which the gene 16S rRNA has a specific base sequence. As the microorganism, Rhodococcus sp. Cr4 strain and Rhodococcus sp. Am8 strain or the like is employed.

摘要翻译： 通过使用基因16S rRNA具有特定碱基序列的新型微生物，将具有复杂结构的腈化合物（例如2-羟基-4-甲基硫代丁腈）转化为高生产效率的酰胺化合物。 作为微生物，红球菌属 Cr4菌株和红球菌属 使用Am8菌株等。

公开(公告)号：US06261798B1

公开(公告)日：2001-07-17

申请号：US09582171

申请日：2000-06-22

申请人： Osamu Kato ,  Makoto Kaneko ,  Takakazu Endo

发明人： Osamu Kato ,  Makoto Kaneko ,  Takakazu Endo

IPC分类号： C12P1302

CPC分类号： C12P13/02

摘要： The present invention relates to a method of producing [S,S]-ethylenediamine-N,N′-disuccinate wherein a microorganism having malate isomerase activity or matter processed therefrom and a microorganism having ethylenediamine-N,N′-disuccinate ethylenediamine lyase activity or matter processed therefrom are allowed to act on a substrate solution containing maleic acid, maleic anhydride, or a maleic acid salt, and ethylenediamine, in the presence of at least one metal ion selected from the group consisting of alkaline earth metals, iron, zinc, copper, nickel, aluminum, titanium and manganese. The present invention enables to accumulate [S,S]-ethylenediamine-N,N′-disuccinate in a higher yield and at a high concentration within a reaction system using maleic acid as a raw material.

摘要翻译： 本发明涉及一种生产[S，S] - 乙二胺-N，N'-二琥珀酸盐的方法，其中具有苹果酸异构酶活性的微生物或由其处理的物质和具有乙二胺-N，N'-二琥珀酸乙二胺裂解酶活性的微生物或 在其中处理的物质可以在至少一种选自碱土金属，铁，锌，锌等的金属离子的存在下作用于含有马来酸，马来酸酐或马来酸盐和乙二胺的底物溶液， 铜，镍，铝，钛和锰。 本发明能够以马来酸为原料，在反应体系内以更高的收率和高浓度积累[S，S] - 乙二胺-N，N'-二琥珀酸盐。

公开(公告)号：US06214592B1

公开(公告)日：2001-04-10

申请号：US09000064

申请日：1998-04-03

申请人： Joël Crouzet ,  Olivier Favre-Bulle ,  Catherine Jourdat ,  Anne-Marie Le Coq ,  Dominique Petre

发明人： Joël Crouzet ,  Olivier Favre-Bulle ,  Catherine Jourdat ,  Anne-Marie Le Coq ,  Dominique Petre

IPC分类号： C12P1302

CPC分类号： C12R1/15 ,  C12N9/80

摘要： The present invention relates to an enzyme with amidase activity, particularly towards substrates of the oligomer type derived from PA 6.6 (formula I) and/or PA 6 (formula II), said enzyme being characterized by a ratio Ra =   ⁢ enzymatic ⁢   ⁢ activity ⁢   ⁢ towards ⁢   ⁢ (poly)amide ⁢   ⁢ substrates ⁢   ⁢ of ⁢   ⁢ formula ⁢   ⁢   ⁢ ( I ) ⁢   ⁢     ⁢ enzymatic ⁢   ⁢ activity ⁢   ⁢ towards ⁢   ⁢ (poly)amide ⁢   ⁢ substrates ⁢ of ⁢   ⁢   ⁢ formul ⁢   ⁢ a ⁢   ⁢ ( ⁢ II) ⁢   = E .   ⁢ A .   ⁢ ( I ) E .   ⁢ A .   ⁢ ( II )   which is greater than 2, preferably greater than or equal to 10 and particularly preferably greater than or equal to 50. More precisely, this enzyme consists of the peptide sequence corresponding to the attached sequence SEQ ID NO: 2. The invention further relates to the DNA which codes for this polypeptide SEQ ID NO: 2 and whose sequence corresponds to the attached sequence SEQ ID NO: 1. The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.

摘要翻译： 本发明涉及具有酰胺酶活性的酶，特别是涉及衍生自PA 6.6（式I）和/或PA 6（式II）的低聚物类型的底物，所述酶的特征在于大于2，优选更大 更优选地，该酶由对应于所附连接序列SEQ ID NO：2的肽序列组成。本发明还涉及编码该多肽SEQ ID NO： NO：2，其序列对应于所附序列SEQ ID NO：1。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

公开(公告)号：US06184008B2

公开(公告)日：2001-02-06

申请号：US09336601

申请日：1999-06-21

申请人： Hiromichi Ohta ,  Takeshi Sugai ,  Takeshi Ishii ,  Satoshi Mitsuda

发明人： Hiromichi Ohta ,  Takeshi Sugai ,  Takeshi Ishii ,  Satoshi Mitsuda

IPC分类号： C12P1302

CPC分类号： C07C233/17 ,  C12N9/18 ,  C12P13/02 ,  C12P41/004

摘要： An industrially advantageous production method of an optically active alcohol useful for cosmetics and medical supplies or production intermediates thereof using an esterase having an ability to selectively hydrolyze an optically active sphingoid compound.

摘要翻译： 可用于化妆品和医疗用途的光学活性醇的工业上有利的生产方法或其使用具有选择性水解旋光性类鞘氨醇化合物的能力的酯酶的生产中间体。

公开(公告)号：US06599721B2

公开(公告)日：2003-07-29

申请号：US09938333

申请日：2001-08-23

申请人： Andrew Naughton ,  Nicholas Shaw

发明人： Andrew Naughton ,  Nicholas Shaw

IPC分类号： C12P1302

CPC分类号： C07C235/06 ,  C07B2200/07

摘要： The invention relates to a novel method for the preparation of optically active 3,3,3-trifluoromethyl-2-alkyl propionic acid derivatives of the general formulae (I) and (II), in which R is ethyl or methyl and X is OH or NH2, provided that if R is methyl X≠—OH. The method comprises the reaction of a racemic propionic acid amide of the general formula (III) either by means of microorganisms which are able to use the propionic acid amide in the form of the racemate or one of its optically active isomers as the only nitrogen source or by means of a polypeptide with amidohydrolase activity which is able to hydrolyze the propionic acid amide. The invention also relates to new optically active representatives of this category of compounds.

摘要翻译： 本发明涉及一种制备通式（I）和（II）的光学活性3,3,3-三氟甲基-2-烷基丙酸衍生物的新方法，其中R是乙基或甲基，X是OH 或NH 2，条件是如果R是甲基X≠-OH。 该方法包括通过能够使用外消旋体形式的丙酰胺或其旋光异构体之一的微生物作为唯一的氮源，使通式（III）的外消旋丙酸酰胺反应 或通过能够水解丙酸酰胺的具有酰胺水解酶活性的多肽。 本发明还涉及这类化合物的新的光学活性代表。

公开(公告)号：US06569652B1

公开(公告)日：2003-05-27

申请号：US09486442

申请日：2000-02-25

申请人： Tamotsu Eguchi ,  Hideyo Kumazawa ,  Kenichi Mochida

发明人： Tamotsu Eguchi ,  Hideyo Kumazawa ,  Kenichi Mochida

IPC分类号： C12P1302

CPC分类号： C12P7/22

摘要： The present invention relates to a process for producing optically active diols represented by the general formula (II): wherein R1 represents (CH2)n, CH═CH, O, S or NH whereupon n is an integer of 1 to 4, and R2 represents hydrogen, C1-6 alkyl, C1-6 alkoxy, (C1-6 alkoxy)-carbonyl, hydroxy, carboxy, halogen, nitro or amino, which comprises treating compounds represented by the general formula (I): wherein R1 and R2 have the same meanings as defined above, with a culture of a microorganism belonging to the genus Rhodococcus, Bacillus, Brevibacterium or Gordona and being capable of stereoselectively diolating a double bond in ring A, or with a culture of Mortierella vinacea, or with said microorganism itself, or with a treated material from said microorganism.

摘要翻译： 本发明涉及由通式（II）表示的光学活性二醇的制备方法：其中R1表示（CH2）n，CH = CH，O，S或NH，其中n为1〜4的整数，R2为 代表氢，C 1-6烷基，C 1-6烷氧基，（C 1-6烷氧基） - 羰基，羟基，羧基，卤素，硝基或氨基，其包括处理由通式（I）表示的化合物：其中R1和R2具有 与上述定义相同，具有属于红球菌属，芽孢杆菌属，短杆菌属或戈多纳属的微生物的培养物，并且能够立体选择性地使环A中的双键二醇化，或与被孢霉属（Mortierella vinacea）的培养物或与所述微生物本身 ，或与来自所述微生物的处理物质混合。

公开(公告)号：US06436697B1

公开(公告)日：2002-08-20

申请号：US09425484

申请日：1999-10-22

申请人： Karen Holt ,  Stephen John Clifford Taylor ,  Peter David Tiffin

发明人： Karen Holt ,  Stephen John Clifford Taylor ,  Peter David Tiffin

IPC分类号： C12P1302

CPC分类号： C12P41/007 ,  C07K5/06043 ,  C12P21/02 ,  Y02P20/55

摘要： A process for the preparation of a compound of formula (1) comprises reacting compounds of formulae (2) and (3) in the presence of an enzyme, wherein A is a thiol-protecting group, B, C and D are each the same or different organic groups of up to 30 C atoms, optionally functionalized at any position, provided that neither a primary amine nor a primary amide is present, and X is a group that can be displaced by NH2.

摘要翻译： 制备式（1）化合物的方法包括在酶的存在下使式（2）和（3）的化合物反应，其中A是硫醇保护基，B，C和D各自相同 或至多30个C原子的不同有机基团，任选在任何位置官能化，条件是不存在伯胺和伯酰胺，并且X是可以被NH 2置换的基团。