METHOD FOR INCREASING USE EFFICIENCY OF LIPASE DURING ENZYMATIC REACTION

    公开(公告)号:US20230065361A1

    公开(公告)日:2023-03-02

    申请号:US17876515

    申请日:2022-07-28

    IPC分类号: C12P7/6454

    摘要: The present disclosure discloses a method for increasing the usage efficiency of a lipase in the enzymatic reaction, belonging to the technical field of food processing. In the present disclosure, the peroxide value of raw materials required for enzymatic synthesis of a structured lipid is reduced by a distillation method or an adsorption method, and then the raw material and a lipase undergo catalytic reaction to synthesize the structured lipid, so as to maintain the activity of the lipase and increase the reuse rate of the enzyme. The distillation method includes deodorization and molecular distillation. The present disclosure provides a method for manufacturing a structured lipid by a high efficiency and low-cost enzymatic synthesis, addressing to the issues such as large enzyme consumption, expansive cost, low enzyme utilization rate in the traditional industrial production. Therefore, the method provided by the present disclosure has certain development potential and practical significance.

    Method for Synthesizing Diglyceride

    公开(公告)号:US20220282290A1

    公开(公告)日:2022-09-08

    申请号:US17639703

    申请日:2020-04-21

    IPC分类号: C12P7/6454

    摘要: Disclosed is a method for synthesizing a diglyceride. The diglyceride is obtained by mixing a fatty acid donor with glycerol, partial glyceride lipase, and monoglyceride lipase by adding water, then subjecting the same to an esterification reaction, with a reaction time of 8 to 24 hours, and further separating and purifying the same. In the present invention, monoglyceride lipase is used to promote the reaction efficiency of partial glyceride lipase in the esterification reaction, so as to increase the synthesis rate of diglyceride. Compared with a single enzyme, the synthesis time is shortened by half or more, and 45.50% or more of diglyceride is obtained after the esterification reaction. Since substantially no triglyceride is generated in products, the content of DAG reaches 98% or more after the same has been purified by means of molecular distillation.

    Process for hydrolyzing oil with high melting point by lipase

    公开(公告)号:US12129508B2

    公开(公告)日:2024-10-29

    申请号:US17425692

    申请日:2019-10-02

    IPC分类号: C12P7/6454 C12N9/20

    摘要: The present invention is in the field of oil processing, and specifically the present invention relates to a process of hydrolyzing an oil with high melting point, such as hydrogenated castor oil (HCO), by lipase. In an embodiment of the invention, there is provided a process of hydrolyzing an oil having a higher melting point to obtain a hydrolysis product, the process comprising: a step of mixing water, an organic solvent, and a lipase with the oil to form a mixture, and a step of hydrolyzing the oil in the mixture, wherein the lipase comprises one or more lipase derived from Thermomyces sp. but not any monoacylglycerol lipase or any lipase derived from Rhizomucor sp., and the organic solvent is any one selected from a sterically hindered alcohol, a non-polar organic solvent, and a mixture of a water-miscible organic solvent with a non-polar organic solvent.

    Triglycerides and Structured Lipids from Short- and Medium-Chain Fatty Acids

    公开(公告)号:US20240352492A1

    公开(公告)日:2024-10-24

    申请号:US18634373

    申请日:2024-04-12

    申请人: BioVeritas, LLC

    摘要: A method including reacting carboxylic acids obtained from fermentation and a carboxylic acid recovery step from said fermentation to produce short- or medium-chain triglycerides, wherein the reacting carboxylic acids comprises direct esterification with glycerol in the presence of a catalyst. Such method mentioned above wherein the produced short- or medium-chain triglycerides are further interesterified with an oil, butter, fat or other lipids in the presence of a catalyst to produce structured lipids. Yet another method comprising reacting carboxylic acids obtained from fermentation and a carboxylic acid recovery step from said fermentation to produce structured lipids, wherein reacting the carboxylic acids comprises transesterification with an oil, butter, fat or other lipids in the presence of a catalyst. The use of such short-chain triglycerides, medium-chain triglycerides and structured lipids as nutritional additives, dietary supplements, or both.

    ENZYMATIC SYNTHESIS METHOD OF 1-PALMITIC ACID-2-OLEIC ACID-3-TRIGLYCERIDE STEARATE

    公开(公告)号:US20230227870A1

    公开(公告)日:2023-07-20

    申请号:US17881644

    申请日:2022-08-05

    IPC分类号: C12P7/6454 C12N9/18

    摘要: The present invention discloses an enzymatic synthesis method of 1-palmitic acid-2-oleic acid-3-triglyceride stearate, belonging to the technical field of structured lipid processing. In the present invention, a crude product rich in 1-palmitic acid-2-oleic acid-3-triglyceride stearate is obtained by reacting for 2-10 h at 20-70° C. with 1-palmitic acid-2-diglyceride oleate as an acyl acceptor, stearic acid or a derivative thereof as an acyl donor and a lipase as a catalyst, wherein the reaction system is an inorganic or organic solvent reaction system. The present invention provides a highly efficient enzymatic synthesis method of POS, thereby resolving the disadvantage of low POS content and limited use provided by the current synthesizing product of structured lipid. The technology adopted by the present invention certainly has an application prospect in the process of preparing cocoa butter equivalents.

    METHOD FOR MANUFACTURING SN-2 PALMITIC TRIACYLGLYCEROLS

    公开(公告)号:US20230159966A1

    公开(公告)日:2023-05-25

    申请号:US17995318

    申请日:2021-04-06

    摘要: The present invention concerns an enzymatic process for the preparation of an ingredient comprising 1,3-Olein-2-palmitin (OPO), the most abundant triglyceride present in human breast milk. This is achieved by using immobilized lipase from Ther-momyces lanuginosis for producing 1,3-olein-2-palmitin (1,3-Dioleate-2-palmitate-glycerol) using as substrate tripalmitin or triglycerides enriched in palmitic acid at SN-2 position by first alcoholysis in presence of C3 to C5 alcohol (butanol, pentanol, isopropanol) to produce 2-monopalmitin which is purified by selective crystallization at decreased temperature, followed by esterification using the same lipase and oleic acid.