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公开(公告)号:US20230189834A1
公开(公告)日:2023-06-22
申请号:US17995415
申请日:2021-04-06
发明人: AMAURY PATIN , TIM BÖRNER , LARS DAHLGREN , FRANCESCA GIUFFRIDA
IPC分类号: A23D9/04 , C11C3/00 , C12P7/6454
CPC分类号: A23D9/04 , C11C3/003 , C12P7/6454
摘要: The present invention concerns an enzymatic process for the preparation of a triglyceride ingredient comprising triglycerides present in human breast milk.
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公开(公告)号:US20230065361A1
公开(公告)日:2023-03-02
申请号:US17876515
申请日:2022-07-28
申请人: Jiangnan University
发明人: Xiaosan WANG , Ye HUANG , Zhuoneng HUANG , Ye CHEN , Keying LIU , Yaqi HUANG , Xiaohan WANG , Ming CHANG
IPC分类号: C12P7/6454
摘要: The present disclosure discloses a method for increasing the usage efficiency of a lipase in the enzymatic reaction, belonging to the technical field of food processing. In the present disclosure, the peroxide value of raw materials required for enzymatic synthesis of a structured lipid is reduced by a distillation method or an adsorption method, and then the raw material and a lipase undergo catalytic reaction to synthesize the structured lipid, so as to maintain the activity of the lipase and increase the reuse rate of the enzyme. The distillation method includes deodorization and molecular distillation. The present disclosure provides a method for manufacturing a structured lipid by a high efficiency and low-cost enzymatic synthesis, addressing to the issues such as large enzyme consumption, expansive cost, low enzyme utilization rate in the traditional industrial production. Therefore, the method provided by the present disclosure has certain development potential and practical significance.
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公开(公告)号:US11718837B2
公开(公告)日:2023-08-08
申请号:US17255831
申请日:2020-07-29
申请人: AMANO ENZYME INC.
发明人: Tomohiro Kimura , Tomoya Ryoki , Yusuke Tanaka , Naoto Katsumata , Yukihide Sato
IPC分类号: C12N9/20 , C12P7/64 , C12P7/6454
CPC分类号: C12N9/20 , C12P7/64 , C12P7/6454 , C12Y301/01003
摘要: To find a novel lipase suitable for processing of oils and fats and to use and apply the lipase. Provided are a novel lipase including an amino acid sequence having 90% or more identity with an amino acid sequence of SEQ ID NO: 1 or 2, and an enzyme preparation containing the same as an active ingredient. The enzyme preparation is used for modifying and improving the physical properties of oils and fats.
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公开(公告)号:US20230033620A1
公开(公告)日:2023-02-02
申请号:US17757711
申请日:2020-12-17
发明人: Stefan Julian Liebig , Jan Marian von Hof , Thomas Böhmer , Thomas Thomalla , Kathrin Daniela Brandt , Christian Hartung , Hans Henning Wenk , Maxim Yavorsky , Sunay Karacocuk
IPC分类号: C12P7/6454 , C12P7/62
摘要: Xylitol carboxylates are products of interest in the food and cosmetics industries. A process can be used for the enzymatic preparation of xylitol carboxylates.
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公开(公告)号:US20220282290A1
公开(公告)日:2022-09-08
申请号:US17639703
申请日:2020-04-21
发明人: Yonghua Wang , Xuan Liu , Bo Yang , Weifei Wang , Dongming Lan , Riming Luo
IPC分类号: C12P7/6454
摘要: Disclosed is a method for synthesizing a diglyceride. The diglyceride is obtained by mixing a fatty acid donor with glycerol, partial glyceride lipase, and monoglyceride lipase by adding water, then subjecting the same to an esterification reaction, with a reaction time of 8 to 24 hours, and further separating and purifying the same. In the present invention, monoglyceride lipase is used to promote the reaction efficiency of partial glyceride lipase in the esterification reaction, so as to increase the synthesis rate of diglyceride. Compared with a single enzyme, the synthesis time is shortened by half or more, and 45.50% or more of diglyceride is obtained after the esterification reaction. Since substantially no triglyceride is generated in products, the content of DAG reaches 98% or more after the same has been purified by means of molecular distillation.
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公开(公告)号:US12129508B2
公开(公告)日:2024-10-29
申请号:US17425692
申请日:2019-10-02
IPC分类号: C12P7/6454 , C12N9/20
CPC分类号: C12P7/6454 , C12N9/20 , C12Y301/01003
摘要: The present invention is in the field of oil processing, and specifically the present invention relates to a process of hydrolyzing an oil with high melting point, such as hydrogenated castor oil (HCO), by lipase. In an embodiment of the invention, there is provided a process of hydrolyzing an oil having a higher melting point to obtain a hydrolysis product, the process comprising: a step of mixing water, an organic solvent, and a lipase with the oil to form a mixture, and a step of hydrolyzing the oil in the mixture, wherein the lipase comprises one or more lipase derived from Thermomyces sp. but not any monoacylglycerol lipase or any lipase derived from Rhizomucor sp., and the organic solvent is any one selected from a sterically hindered alcohol, a non-polar organic solvent, and a mixture of a water-miscible organic solvent with a non-polar organic solvent.
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公开(公告)号:US20240352492A1
公开(公告)日:2024-10-24
申请号:US18634373
申请日:2024-04-12
申请人: BioVeritas, LLC
发明人: Cesar Granda , Jubo Zhang
IPC分类号: C12P7/6454 , C12N9/16 , C12N9/20 , C12P7/6458
CPC分类号: C12P7/6454 , C12N9/16 , C12N9/20 , C12P7/6458 , C12Y301/01003 , C12Y301/01023
摘要: A method including reacting carboxylic acids obtained from fermentation and a carboxylic acid recovery step from said fermentation to produce short- or medium-chain triglycerides, wherein the reacting carboxylic acids comprises direct esterification with glycerol in the presence of a catalyst. Such method mentioned above wherein the produced short- or medium-chain triglycerides are further interesterified with an oil, butter, fat or other lipids in the presence of a catalyst to produce structured lipids. Yet another method comprising reacting carboxylic acids obtained from fermentation and a carboxylic acid recovery step from said fermentation to produce structured lipids, wherein reacting the carboxylic acids comprises transesterification with an oil, butter, fat or other lipids in the presence of a catalyst. The use of such short-chain triglycerides, medium-chain triglycerides and structured lipids as nutritional additives, dietary supplements, or both.
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公开(公告)号:US20230227870A1
公开(公告)日:2023-07-20
申请号:US17881644
申请日:2022-08-05
申请人: Jiangnan University
发明人: Xiaosan WANG , Ye HUANG , Yifan WANG , Keying LIU , Ye CHEN
IPC分类号: C12P7/6454 , C12N9/18
CPC分类号: C12P7/6454 , C12N9/18 , C12Y301/01003
摘要: The present invention discloses an enzymatic synthesis method of 1-palmitic acid-2-oleic acid-3-triglyceride stearate, belonging to the technical field of structured lipid processing. In the present invention, a crude product rich in 1-palmitic acid-2-oleic acid-3-triglyceride stearate is obtained by reacting for 2-10 h at 20-70° C. with 1-palmitic acid-2-diglyceride oleate as an acyl acceptor, stearic acid or a derivative thereof as an acyl donor and a lipase as a catalyst, wherein the reaction system is an inorganic or organic solvent reaction system. The present invention provides a highly efficient enzymatic synthesis method of POS, thereby resolving the disadvantage of low POS content and limited use provided by the current synthesizing product of structured lipid. The technology adopted by the present invention certainly has an application prospect in the process of preparing cocoa butter equivalents.
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公开(公告)号:US20230159966A1
公开(公告)日:2023-05-25
申请号:US17995318
申请日:2021-04-06
发明人: AMAURY PATIN , TIM BÖRNER , LARS DAHLGREN
IPC分类号: C12P7/6454 , C12P7/6458 , C12N9/20
CPC分类号: C12P7/6454 , C12N9/20 , C12P7/6458 , C12Y301/01003
摘要: The present invention concerns an enzymatic process for the preparation of an ingredient comprising 1,3-Olein-2-palmitin (OPO), the most abundant triglyceride present in human breast milk. This is achieved by using immobilized lipase from Ther-momyces lanuginosis for producing 1,3-olein-2-palmitin (1,3-Dioleate-2-palmitate-glycerol) using as substrate tripalmitin or triglycerides enriched in palmitic acid at SN-2 position by first alcoholysis in presence of C3 to C5 alcohol (butanol, pentanol, isopropanol) to produce 2-monopalmitin which is purified by selective crystallization at decreased temperature, followed by esterification using the same lipase and oleic acid.
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公开(公告)号:US20240270676A1
公开(公告)日:2024-08-15
申请号:US18570018
申请日:2022-05-30
发明人: Stefan Julian Liebig , Jan Marian von Hof , Tobias Karl Hubert Müller , Maxim Yavorsky , Sunay Karacocuk , Alexandra Trambitas , Dominik Schuch
IPC分类号: C07C69/33 , C12P7/6454
CPC分类号: C07C69/33 , C12P7/6454
摘要: n-Nonanoic esters of xylitol, sorbitol or erythritol are made, especially for cosmetic or household care compositions, and a process for their preparation is developed.
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