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公开(公告)号：US07645070B2
公开(公告)日：2010-01-12
申请号：US11804298
申请日：2007-05-17
申请人： John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人： John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号： B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要： An instrument for performing highly accurate PCR employing an assembly, a heated cover, and an internal computer, is provided. The assembly is made up of a sample block, a number of Peltier thermal electric devices, and a heat sink, clamped together. A control algorithm manipulates the current supplied to thermoelectric coolers such that the dynamic thermal performance of a block can be controlled so that pre-defined thermal profiles of sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics.
摘要翻译： 提供了一种使用组件，加热盖和内部计算机执行高精度PCR的仪器。 组件由样品块，多个珀耳帖热电器件和散热器组成，夹在一起。 控制算法控制提供给热电冷却器的电流，使得可以控制块的动态热性能，从而可以执行样本温度的预定义热分布。 计算样品温度，而不是使用设计特定的模型和方程来测量。 控制软件包括校准诊断，其允许从仪器到仪器的热电冷却器的性能的变化被补偿，使得所有仪器执行相同的操作。 块/散热器组件可以改变为相同或不同设计的另一个。 该组件携带在板载存储器件中表征其自身性能所需的必要信息，允许组件在仪器之间可互换，同时保持其精密操作特性。
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