公开(公告)号：US20240361310A1

公开(公告)日：2024-10-31

申请号：US18734732

申请日：2024-06-05

申请人： Zafrens Inc.

发明人： Kandaswamy Vijayan ,  Ravi Ramenani ,  Chulmin Choi ,  Aurelien Laguerre ,  Gopichandran Ravichandran ,  Nicholas Sam-Soon ,  Yi Zhang ,  David Milliken ,  Kenneth Chng ,  Maina Ndungu

IPC分类号： G01N33/545 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/545 ,  G01N33/5308 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/588

摘要： Disclosed are capture beads that are suitable for use in an assay device which is configured to correlate the capture bead to a specific examination area in the device as well as correlating any captured nucleic acid or other cellular components generated during the assay to that bead.

公开(公告)号：US20240353413A1

公开(公告)日：2024-10-24

申请号：US18636321

申请日：2024-04-16

申请人： LEICA MICROSYSTEMS CMS GMBH

发明人： Soeren ALSHEIMER

IPC分类号： G01N33/58 ,  G01N21/65

CPC分类号： G01N33/582 ,  G01N21/65 ,  G01N2021/655

摘要： A label for marking a target structure includes at least one polyyne and at least one oligonucleotide. The at least one polyyne is configured to be detectable via a distinct Raman spectroscopy characteristic.

公开(公告)号：US20240345099A1

公开(公告)日：2024-10-17

申请号：US18683332

申请日：2022-08-11

申请人： Cenios GmbH

发明人： Mohammad SALEHI ,  Angela HAMANN-STEINMEIER

IPC分类号： G01N33/68 ,  B82Y5/00 ,  B82Y15/00 ,  G01N21/64 ,  G01N21/65 ,  G01N33/58

CPC分类号： G01N33/6851 ,  B82Y5/00 ,  B82Y15/00 ,  G01N21/6428 ,  G01N21/658 ,  G01N33/587 ,  G01N2021/6439

摘要： The invention relates to a method for the absolute quantification of at least one analyte by means of a rapid test, in particular by spectroscopic determination. In order to provide a method and a device for the absolute quantification of analytes and to enable the creation of a database for the improved individual dosing of therapeutics, whereby a particularly accurate quantification of at least one analyte is possible in a simple manner, particularly inexpensively, particularly quickly and at any location without the need for a laboratory, it is provided, that at least one nanomaterial is used as a luminescent substance for quantification, which interacts directly or indirectly with the analyte, wherein at least one signal generated by the nanomaterial is measured and wherein in addition an internal and/or external standard series, in particular by means of Raman-labeled targets and/or fluorescence-labeled targets, is measured and subsequently the generated signal is standardized or referenced on the basis of the internal and/or external standard series, so that an absolute quantification is achieved.

公开(公告)号：US20240345094A1

公开(公告)日：2024-10-17

申请号：US18592661

申请日：2024-03-01

申请人： Saint Louis University

发明人： James Edwards ,  Christopher K. Arnatt ,  Michael Armbruster

IPC分类号： G01N33/58 ,  G01N30/02 ,  G01N30/72

CPC分类号： G01N33/58 ,  G01N30/7233 ,  G01N2030/027 ,  G01N2458/15

摘要： The present disclosure provides constant-mass isobaric tags and methods for their use in multiplexed, double fragmentation mass spectrometry. The tags can be used for various aldehyde-, thiol-, amine-, and acid-containing analytes and can be used to quantify their relative amounts in different samples. Up to 192 samples can be combined for analysis.

公开(公告)号：US20240345072A1

公开(公告)日：2024-10-17

申请号：US18409399

申请日：2024-01-10

申请人： Standard BioTools Canada Inc.

发明人： Olga Ornatsky ,  Qing Chang ,  Bedilu Allo ,  Xudong Lou ,  Alexander Bouzekri

IPC分类号： G01N33/532 ,  C07K1/13 ,  G01N33/58

CPC分类号： G01N33/532 ,  C07K1/13 ,  G01N33/583

摘要： The present disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.

公开(公告)号：US20240344120A1

公开(公告)日：2024-10-17

申请号：US18567061

申请日：2021-06-07

申请人： HITACHI HIGH-TECH CORPORATION

发明人： Junko TANAKA ,  Tatsuo NAKAGAWA ,  Takeshi ISHIDA ,  Yoshio KAMURA

IPC分类号： C12Q1/686 ,  G01N21/64 ,  G01N33/58

CPC分类号： C12Q1/686 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6439

摘要： The present invention provides a DNA detection method comprising: a first step of dividing a specimen solution containing a fluorescent-labeled probe or a DNA intercalator and multiple types of DNA to be detected into a plurality of aliquots; a second step of performing PCR in microcompartments, each containing one of the aliquots; a third step of measuring fluorescence intensity from the fluorescent-labeled probe or the DNA intercalator in each of the microcompartments as temperature changes; a fourth step of calculating a melting temperature of the multiple type of DNA to be detected from each measured fluorescence intensity; a fifth step of identifying any microcompartment affected by one or more bubbles; and a sixth step of excluding data obtained for the microcompartment affected by one or more bubbles from all data obtained for the plurality of microcompartments.

公开(公告)号：US12117450B2

公开(公告)日：2024-10-15

申请号：US17292955

申请日：2019-11-15

申请人： Ohio State Innovation Foundation

发明人： Jessica Winter ,  Elizabeth Jergens ,  Kil Ho Lee

IPC分类号： C12Q1/68 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/585 ,  G01N33/54393 ,  G01N2458/10

摘要： Disclosed herein are erasable label systems that involve nanocage molecules positioned around nanoparticles, which can be loaded with, bound to, or adsorbed with imaging agents. The nanocages can contain targeting arms composed of ssDNA or ssRNA that can be used to target biomolecules. For DNA or RNA targeting, this can be done directly. Antibodies can be targeted using avidin-biotin coupling to ssDNA or direct ssDNA conjugation to the antibody surface. ssDNA or ssRNA complementary to one of the arms can then be used to “erase” the label.

公开(公告)号：US20240336617A1

公开(公告)日：2024-10-10

申请号：US18611505

申请日：2024-03-20

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： E. James Petersson ,  Venkatesh Yarra

IPC分类号： C07D487/04 ,  A61K31/4162 ,  A61K31/5377 ,  A61K31/5415 ,  G01N21/64 ,  G01N33/58

CPC分类号： C07D487/04 ,  A61K31/4162 ,  A61K31/5377 ,  A61K31/5415 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6439

摘要： In one aspect, the present disclosure relates to compounds of Formula (I). In certain embodiments, the compounds of Formula (I) comprise fluorescent dyes. In another aspect, the present disclosure provides a method for diagnosing a protein aggregation disease in a subject. In certain embodiments, the protein aggregation disease is selected from the group consisting of Parkinson's disease (PD) and Alzheimer's disease (AD).

公开(公告)号：US12110522B2

公开(公告)日：2024-10-08

申请号：US16962792

申请日：2019-01-11

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Tess Branon ,  Alice Y. Ting

IPC分类号： C12N9/00 ,  G01N33/58 ,  G01N33/68

CPC分类号： C12N9/93 ,  C12Y603/04015 ,  G01N33/58 ,  G01N33/6842

摘要： Engineered promiscuous biotin ligases and methods of using them in proximity labeling are described. In particular, the invention provides novel biotin ligase variants having increased promiscuous biotinylation activity capable of proximity labeling of proteins in live cells in as little as 10 minutes.

公开(公告)号：US20240329060A1

公开(公告)日：2024-10-03

申请号：US18742611

申请日：2024-06-13

申请人： SYSMEX CORPORATION

发明人： Igor KUROCHKIN ,  Lausonia RAMASWAMY

IPC分类号： G01N33/68 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/6896 ,  G01N33/543 ,  G01N33/58 ,  G01N2333/33 ,  G01N2333/4703 ,  G01N2333/4709 ,  G01N2333/70596 ,  G01N2333/916 ,  G01N2800/28

摘要： Disclosed is a method for measuring neuron-derived extracellular vesicles in a biological sample in vitro, the method comprising: forming a complex comprising a capture body comprising a tetanus toxin C-terminal fragment, the extracellular vesicle, a detector that specifically binds to a target molecule of the extracellular vesicle, and a labeling substance on a solid phase; and measuring extracellular vesicles having the target molecule based on a signal generated by the labeling substance comprised in the complex.