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    METHOD OF DETECTING APOPTOSIS INDUCTION BY MEASURING THE RELEASE OF SUBSTANCES FROM CELL ORGANELLES BY MEANS OF FLOW CYTOMETRY
    1.
    发明申请
    METHOD OF DETECTING APOPTOSIS INDUCTION BY MEASURING THE RELEASE OF SUBSTANCES FROM CELL ORGANELLES BY MEANS OF FLOW CYTOMETRY 审中-公开
    通过流式细胞仪测定从细胞有机物释放物质来检测疫苗诱导的方法

    公开(公告)号：WO2003042703A1

    公开(公告)日：2003-05-22

    申请号：PCT/EP2002/012699

    申请日：2002-11-13

    Applicant: DEBATIN, Klaus-Michael ,  STAHNKE, Karsten ,  HUG, Hubert

    Inventor： DEBATIN, Klaus-Michael ,  STAHNKE, Karsten ,  HUG, Hubert

    IPC: G01N33/68

    CPC classification number: G01N33/5076 ,  G01N33/5079 ,  G01N2510/00

    Abstract: The invention is directed to a method of detecting the release of substances from cell organelles by means of flow cytometry, com rising the steps of: fixing cells to be studied; permeabilizing the outer cell membrane; staining the cells with a fluorochrome compound which binds specifically to at least one substance to be studied; measuring the individual cells by means of flow cytometry while stimulating the fluorochrome compound; and determining the fluorescent strength of the cells.

    Abstract translation: 本发明涉及一种通过流式细胞术检测细胞器物质释放的方法，其特征在于，提高了以下步骤：将待研究的细胞透化外细胞膜，用特异性结合的荧光染料染色细胞 至少一种待研究的物质通过流式细胞术测量各个细胞，同时刺激荧光染料化合物并测定细胞的荧光强度。

    METHOD FOR THE SIMULTANEOUS DETERMINATION OF TWO FLUORESCENT EMISSIONS WITH A SINGLE LASER FLOW CYTOMETER
    2.
    发明申请
    METHOD FOR THE SIMULTANEOUS DETERMINATION OF TWO FLUORESCENT EMISSIONS WITH A SINGLE LASER FLOW CYTOMETER 审中-公开
    步骤中使用一个EINLASER细胞计数仪两种荧光排放同时测定

    公开(公告)号：WO0240978A3

    公开(公告)日：2002-11-28

    申请号：PCT/DE0104125

    申请日：2001-10-30

    Applicant: DEUTSCHES KREBSFORSCH ,  DEBATIN MICHAEL ,  STAHNKE KARSTEN ,  HUG HUBERT

    Inventor： DEBATIN MICHAEL ,  STAHNKE KARSTEN ,  HUG HUBERT

    IPC: G01N15/14 ,  G01N21/64 ,  G01N15/10

    CPC classification number: G01N21/6428 ,  G01N15/14 ,  G01N2021/6441

    Abstract: In flow cytometry, the problem occurs that it is desirable to simultaneously determine two fluorochromes, with overlapping peak emissions. The above is achieved by means of a single laser flow cytometer with two fluorescent channels of various receiver wavelengths in a method comprising the following steps: A) stimulation of the first fluorochrome of reference cells marked with said fluorochrome, by means of a laser beam suitable for both fluorochromes; B) adjustment of the photomultiplier tube voltage in the second fluorescence channel, set for the emissions from the second fluorochrome, to a value at which no fluorescence emission signal is detected in the second fluorescence channel as a result of the first fluorochrome; C) stimulation of the fluorochrome of the sample cells, marked with the first and second fluorochrome by means of the laser beam; D) measurement of the fluorescence emission signal of the first fluorochrome in the sample cells in the first fluorescence channel set for the emissions of the first fluorochrome and E) measurement of the fluorescence emission signal of the second fluorochrome in the sample cells in the second fluorescence channel.

    Abstract translation: 在流式细胞仪，还有就是你要测量两种荧光染料同时用重叠的发射峰值的问题。 这是通过一个单一的激光流的辅助细胞仪与不同的接收波面积的两个荧光通道实现，该方法包括以下步骤：a）激动人心标记与两种荧光染料的合适的激光束此荧光染料的参考单元中的第一荧光染料; B）调节第二荧光通道，其适合于所述第二荧光染料，其中没有通过在第二荧光通道荧光发射信号中的第一荧光色素产生的被检测到的值的发射的光电倍增管电压; C）激动人心不必用激光束来测量第一和第二荧光染料标记的细胞的荧光染料; D）测量所述细胞中的荧光染料的第一的荧光发射信号，以在其中适于将第一荧光染料的发射所述第一信道的荧光来测量; 和e）测量所述第二荧光染料对细胞的荧光发射信号，以在所述第二荧光通道进行测量。

    ASSAYS FOR DETECTING SUBSTANCES WITH AN ANTI-HIV ACTION
    3.
    发明申请
    ASSAYS FOR DETECTING SUBSTANCES WITH AN ANTI-HIV ACTION 审中-公开
    测定法搜索具有抗HIV活性剂

    公开(公告)号：WO9947696A2

    公开(公告)日：1999-09-23

    申请号：PCT/EP9901512

    申请日：1999-03-09

    Applicant: BAYER AG ,  WEBER OLAF ,  HUG HUBERT

    Inventor： WEBER OLAF ,  HUG HUBERT

    IPC: C12Q1/00 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/15 ,  G01N33/50 ,  G01N33/569

    CPC classification number: G01N33/5008 ,  C12Q1/6897 ,  C12Q1/70 ,  G01N2510/00

    Abstract: The invention relates to new assays for detecting substances influencing apoptosis for a new treatment of the disease caused by the human immunodeficiency virus (HIV). The invention is based on the discovery in the human immunodeficiency virus (HIV) of a new region homologous to the "death effector domain" (DED) and on a new antiviral strategy, based on said discovery, for eliminating HIV-infected cells from the body and preventing the acquired immune deficiency syndrome (AIDS).

    Abstract translation: 本发明涉及用于检测细胞凋亡影响的物质用于由人免疫缺陷病毒的新疗法新颖测定（HIV）引起的疾病。 本发明是一种新的“死亡效应域”（DED）的发现同系物的人类免疫缺陷病毒（HIV）的区域，并基于对消除身体和预防获得性免疫缺陷综合征的HIV感染细胞中，这种新的抗病毒策略（AIDS） 根据。

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