Abstract:
Gegenstand der Erfindung ist ein Verfahren umfassend eine Kultivierung von Knallgasbakterien unter niedriger Gelöstsauerstoffkonzentration von 0,001 mg/L bis 0,5 mg/L. Das Verfahren kann ebenfalls die Synthese von Zielprodukten umfassen, welche ausgewählt sind aus Substanzen, deren Bildung über das Zwischenprodukt Acetyl-Coenzym A oder Metabolite des Zitronensäurezyklus erfolgt, z.B. Fettsäuren, Fettsäureester, Fettalkohole, Fettaldehyde, Butanol, Buten, Propen, Aceton, 2-Hydroxyisobuttersäure oder 2-Propanol.
Abstract:
The present invention relates to a method of producing at least one amino acid from a carbon source in aerobic conditions, the method comprising: (a)step of producing ethanol and/or acetate from the carbon source in aerobic conditions, comprising (i)contacting a reaction mixture comprising -a first acetogenic microorganism in an exponential growth phase; -free oxygen; and -a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol; and (b)step of contacting the acetate and/or ethanol from step (a) with a third microorganism capable of converting the acetate and/or ethanol to at least one amino acid.
Abstract:
There is provided a method of producing at least one rhamnolipid comprising: (a) contacting a recombinant cell with a medium containing a carbon source; wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E 1 , E 2 and E 3 , wherein the enzyme E 1 is an α/ß hydrolase ( RHIA ), the enzyme E 2 is a rhamnosyltransferase I ( RHIB ) and the enzyme E 3 is a rhamnosyltransferase II ( RHIC ), and wherein the carbon source is an alkane and/or alkanoic acid comprising 6 to 10 carbon atoms.
Abstract:
The present invention relates to a method of extracting an alkanoic acid and/or ester there of from an aqueous medium, the method comprising : (a)contacting the alkanoic acid and/or ester thereof in the aqueous medium with at least one extracting medium for a time sufficient to extract the alkanoic acid and/or ester thereof from the aqueous medium into the extracting medium, (b)separating the extracting medium with the extracted alkanoic acid and/or ester thereof from the aqueous medium wherein the extracting medium comprises: -a mixture of at least one alkyl-phosphine oxide, preferably Trioctylphosphine oxide (TOPO), and at least one alkane wherein the alkane comprises at least 12 carbon atoms.
Abstract:
The present invention relates to a method of producing at least one alcohol from a carbon source, the method comprising: (a) producing the alcohol in an aqueous medium under aerobic conditions; and (b) extracting the alcohol from step (a) from the aqueous medium by: (bi) contacting the alcohol in the aqueous medium with at least one extracting medium for a time sufficient to extract the alcohol from the aqueous medium into the extracting medium, (bii) separating the extracting medium with the extracted alcohol from the aqueous medium wherein the extracting medium comprises: - oleyl alcohol; or - polypropylene glycol and an alkane wherein the alcohol comprises at least 3 carbon atoms.
Abstract:
The present invention relates to a method of producing propanol and/or propionic acid, the method comprising: (b) contacting at least one propionogen to carbon dioxide and to an aqueous medium comprising ethanol and acetate; wherein the acetate is maintained at a concentration of at least about 1 g/L in the aqueous medium.
Abstract:
The present invention relates to a fermentation medium comprising: (a)Magnesium at a concentration greater than 40mg/L of the fermentation medium, (b)Sulphur at a concentration greater than 85mg/L of the fermentation medium, (c)Aluminium at a detectable concentration, (d)Boron at a detectable concentration, (e)Potassium at a concentration greater than 100mg/L of the fermentation medium, or combinations thereof and a method of using this formation medium for producing at least one alcohol from a carbon source.
Abstract:
The present invention relates to a compound of general formula (I). The present invention also relates to a method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of (a) an enzyme E 1 ,a homoserine dehydrogenase (EC1.1.1.3) and/or an enzyme E 5 , an aspartokinase (EC2.7.2.4); and (b) an enzyme E 2 , a homoserine O-acetyl transferase (EC2.3.1.31) and the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.
Abstract:
The present invention relates to a method of producing at least one alcohol from a carbon source, the method comprising: (a) producing the alcohol in an aqueous medium under aerobic conditions; and (b) extracting the alcohol from step (a) from the aqueous medium by: (bi) contacting the alcohol in the aqueous medium with at least one extracting medium for a time sufficient to extract the alcohol from the aqueous medium into the extracting medium, (bii )separating the extracting medium with the extracted alcohol from the aqueous medium wherein the extracting medium comprises: -oleyl alcohol; or -polypropylene glycol and an alkane wherein the alcohol comprises at least 3 carbon atoms.
Abstract:
The present invention relates to a method of producing valeric acid, heptanoic acid, esters and/or salts thereof from a carbon source, the method comprising a step of contacting at least one microorganism with the carbon source in an aqueous medium, wherein the carbon source is ethanol and propionic acid and the concentration of propionic acid is about ≤ 10g/L.