公开(公告)号：WO2018100370A1

公开(公告)日：2018-06-07

申请号：PCT/GB2017/053603

申请日：2017-11-29

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： CLARKE, James ,  WHITE, James ,  MUSCAT, Richard ,  KNOT, Jessica Mary May ,  NATHANI, Ramiz Iqbal ,  HERON, Andrew John ,  BRUCE, Mark John ,  JAYASINGHE, Lakmal Nishantha ,  CAPROTTI, Domenico ,  STODDART, David Jackson ,  BOWEN, Rebecca Victoria ,  WRIGHT, Christopher James ,  MOODY, Paul Richard

IPC: C12Q1/6869

Abstract: Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g ., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g ., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

公开(公告)号：WO2015166276A1

公开(公告)日：2015-11-05

申请号：PCT/GB2015/051291

申请日：2015-05-01

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： JAYASINGHE, Lakmal ,  WALLACE, Elizabeth Jayne ,  PUGH, Jonathan Bankes ,  HAMBLEY, Richard George ,  WOOD, Neil Roger ,  BROWN, Clive Gavin ,  WHITE, James

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C07K14/35 ,  C12Q2563/157 ,  C12Q2565/631

Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.

Abstract translation: 本发明涉及当运动由多核苷酸结合蛋白控制时，改善靶多核苷酸相对于跨膜孔的运动。 本发明还涉及改进的跨膜孔和多核苷酸结合蛋白。

公开(公告)号：WO2014013259A1

公开(公告)日：2014-01-23

申请号：PCT/GB2013/051924

申请日：2013-07-18

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： WHITE, James ,  MOYSEY, Ruth ,  MISCA, Mihaela

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q2522/101 ,  C12Q2523/31 ,  C12Q2565/631

Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.

Abstract translation: 本发明涉及使用单链结合蛋白（SSB）表征靶多核苷酸的方法。 SSB是包含不具有净负电荷的羧基末端（C末端）区域的SSB或包含其C末端区域中的一个或多个修饰的修饰SSB，其降低C-末端区域的净负电荷， 终端区域。

公开(公告)号：WO2013121224A1

公开(公告)日：2013-08-22

申请号：PCT/GB2013/050381

申请日：2013-02-18

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： REID, Stuart William ,  CLARKE, James Anthony ,  WHITE, James ,  HARPER, Gavin

IPC: C12Q1/68 ,  B82Y15/00 ,  G01N33/487 ,  G06F19/22

CPC classification number: G01N33/48792 ,  B82Y15/00 ,  C12Q1/6858 ,  C12Q1/6869 ,  G01N27/02 ,  G01N33/48721 ,  G01N33/6803 ,  G01N33/6875 ,  G06F19/22 ,  C12Q2537/165 ,  C12Q2565/631

Abstract: A time-ordered series of measurements of a polymer made during translocation of the polymer through a nanopore are analysed. The measurements are dependent on the identity of k-mers in the nanopore, a k-mer being k polymer units of the polymer, where k is a positive integer. The method involves deriving, from the series of measurements, a feature vector of time-ordered features representing characteristics of the measurements; and determining similarity between the derived feature vector and at least one other feature vector.

Abstract translation: 分析了在聚合物通过纳米孔的位移期间制备的聚合物的时间有序的一系列测量。 测量取决于纳米孔中的k-mers的同一性，k-mer是聚合物的聚合物单元，其中k是正整数。 该方法包括从一系列测量中导出表示测量特征的时间有序特征的特征向量; 以及确定所导出的特征向量与至少一个其他特征向量之间的相似度。

公开(公告)号：WO2010086602A1

公开(公告)日：2010-08-05

申请号：PCT/GB2010/000132

申请日：2010-01-29

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED ,  JAYASINGHE, Lakmal ,  MILTON, John ,  MCNEILL, Luke ,  CLARKE, James ,  WHITE, James ,  MOYSEY, Ruth

Inventor： JAYASINGHE, Lakmal ,  MILTON, John ,  MCNEILL, Luke ,  CLARKE, James ,  WHITE, James ,  MOYSEY, Ruth

IPC: C07K19/00 ,  C12N15/11

CPC classification number: C12N9/22 ,  C07K14/31 ,  C07K2319/03 ,  C12N9/1241 ,  C12N9/90

Abstract: The invention provides method of covalently coupling two or more moieties, the method comprising: (a) providing a first moiety having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond; (b) providing a second moiety having covalently attached thereto (i) at least one second linker comprising a second hybridizable region capable of hybridizing to the first hybridizable region and (ii) at least a second group capable of forming a covalent bond with the first group; (c) contacting the first and second moieties under conditions that allow the first and second hybridizable regions to hybridize and link the moieties; and (d) exposing the linked moieties to conditions that allow the formation of a covalent bond between the first and second groups.

Abstract translation: 本发明提供共价偶联两个或多个部分的方法，所述方法包括：（a）提供共价连接到其上的第一部分（i）至少一个包含第一可杂交区域的第一接头和（ii）至少一个能够 形成共价键; （b）提供共价连接到其上的第二部分（i）至少一个第二接头，其包含能够与第一可杂交区域杂交的第二可杂交区域和（ii）至少第二个能够与第一可共< 组; （c）在允许第一和第二可杂交区域杂交并连接部分的条件下使第一和第二部分接触; 和（d）将连接的部分暴露于允许在第一和第二基团之间形成共价键的条件。

公开(公告)号：WO2010004265A1

公开(公告)日：2010-01-14

申请号：PCT/GB2009/001679

申请日：2009-07-06

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED ,  JAYASINGHE, Lakmal ,  BAYLEY, Hagan ,  CHELEY, Stephen ,  MCKEOWN, Brian ,  WHITE, James ,  CLARKE, James

Inventor： JAYASINGHE, Lakmal ,  BAYLEY, Hagan ,  CHELEY, Stephen ,  MCKEOWN, Brian ,  WHITE, James ,  CLARKE, James

IPC: C12N9/12 ,  C12N9/22 ,  C12N9/52 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C07K14/31 ,  C12N9/1247 ,  C12N9/1252 ,  C12N9/127 ,  C12N9/1276 ,  C12N9/16 ,  C12N9/22 ,  C12N9/52 ,  C12N9/90 ,  C12N9/96 ,  C12Q2565/631

Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

Abstract translation: 本发明涉及包含跨膜蛋白质孔亚基和核酸处理酶的构建体。 孔亚基共价连接到酶上，使得亚基和酶保持其活性。 构建体可用于产生具有附着于其上的核酸处理酶的跨膜蛋白孔。 这样的孔特别可用于测序核酸。 酶以这样的方式处理核酸，使得孔可以通过随机感测检测其成分核苷酸。

公开(公告)号：WO2021255477A1

公开(公告)日：2021-12-23

申请号：PCT/GB2021/051557

申请日：2021-06-18

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： GRAHAM, James Edward ,  YOUD, Christopher Peter ,  BROWN, Clive Gavin ,  CRAWFORD, Marion Louise ,  HERON, Andrew John ,  WHITE, James

IPC: C12Q1/6869

Abstract: Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.

公开(公告)号：WO2021255476A2

公开(公告)日：2021-12-23

申请号：PCT/GB2021/051556

申请日：2021-06-18

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： BOWEN, Rebecca Victoria ,  BROWN, Clive Gavin ,  BRUCE, Mark John ,  GARALDE, Daniel Ryan ,  GRAHAM, James Edward ,  HERON, Andrew John ,  RAIMONDEAU, Etienne ,  WHITE, James ,  YOUD, Christopher Peter

IPC: C12Q1/6869

Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

公开(公告)号：WO2019234432A1

公开(公告)日：2019-12-12

申请号：PCT/GB2019/051571

申请日：2019-06-06

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： CLARKE, James Anthony ,  WHITE, James ,  MUSCAT, Richard ,  JOHNSON, Jessica Mary May ,  NATHANI, Ramiz Iqbal ,  HERON, Andrew John ,  BRUCE, Mark John ,  JAYASINGHE, Lakmal Nishantha ,  CAPROTTI, Domenico ,  STODDART, David Jackson ,  BOWEN, Rebecca Victoria ,  WRIGHT, Christopher James ,  MOODY, Paul Richard

IPC: C12Q1/6869

Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

公开(公告)号：WO2017203269A1

公开(公告)日：2017-11-30

申请号：PCT/GB2017/051493

申请日：2017-05-25

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： HERON, Andrew John ,  GARALDE, Daniel Ryan ,  WHITE, James

IPC: C12Q1/68

Abstract: The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.

Abstract translation: 本发明涉及使用孔来表征两个或更多个靶多核苷酸的新方法。 该方法涉及将第一多核苷酸依次连接一个或多个后续多核苷酸以形成连接多核苷酸。