公开(公告)号：WO0073413A9

公开(公告)日：2002-01-24

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。